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Evaluation of ATP Bioluminescence Assays for Potential Use in a Hospital Setting

Published online by Cambridge University Press:  02 January 2015

Zoie A. Aiken*
Affiliation:
Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom
Michael Wilson
Affiliation:
Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom
Jonathan Pratten
Affiliation:
Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom
*
Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WCIX 8LD, United Kingdom, (z.aiken@eastman.ucl.ac.uk)

Extract

ATP bioluminescence is being applied in hospitals to measure surface contamination. We compared commercial luminometers for detecting the number Staphylococcus aureus associated with surfaces. The data showed that the ATP bioluminescence methods tested were not robust enough to generate quantitative data on bacterial numbers, especially at low concentrations.

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2011

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References

1. Hota, B. Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clin Infect Dis 2004;39:11821189.Google Scholar
2. Boyce, JM, Havill, NL, Otter, JA, Adams, NM. Widespread environmental contamination associated with patients with diarrhea and methicillin-resistant Staphylococcus aureus colonization of the gastrointestinal tract. Infect Control Hosp Epidemiol 2007;28:11421147.CrossRefGoogle ScholarPubMed
3. Griffith, CJ, Blucher, A, Fieri, J, Fielding, L. An evaluation of luminometry as a technique in food microbiology and a comparison of 6 commercially available luminometers. Food Sci Technol Today 1994;8:209216.Google Scholar
4. Boyce, JM, Havill, NL, Dumigan, DG, Golebiewski, M, Balogun, O, Rizvani, R. Monitoring the effectiveness of hospital cleaning practices by use of an adenosine triphosphate bioluminescence assay. Infect Control Hosp Epidemiol 2009;30:678684.Google Scholar
5. Lewis, T, Griffith, C, Gallo, M, Weinbren, M. A modified ATP benchmark for evaluating the cleaning of some hospital environmental surfaces. J Hosp Infect 2008;69:156163.Google Scholar
6. Lundin, A. Use of firefly luciferase in ATP-related assays of bio-mass, enzymes, and metabolites. Methods Enzymol 2000;305:346370.Google Scholar
7. Moore, G, Griffith, CJ, Fielding, L. A comparison of traditional and recently developed methods for monitoring surface hygiene within the food industry: a laboratory study. Dairy Food Environ Sanit 2001;21:478488.Google Scholar
8. Poulis, JA, de Pijper, M, Mossel, DA, Dekkers, PP. Assessment of cleaning and disinfection in the food industry with the rapid ATP-bioluminescence technique combined with the tissue fluid contamination test and a conventional microbiological method. Int J Food Microbiol 1993;20:109116.CrossRefGoogle Scholar
9. Dancer, SJ. How do we assess hospital cleaning? a proposal for microbiological standards for surface hygiene in hospitals. J Hosp Infect 2004;56:1015.CrossRefGoogle ScholarPubMed
10. Moore, G, Griffith, C. A comparison of traditional and recendy developed methods for monitoring surface hygiene within the food industry: an industry trial. Int J Environ Health Res 2002;12:317.Google Scholar