Infection Control & Hospital Epidemiology

Concise Communications

Evaluation of ATP Bioluminescence Assays for Potential Use in a Hospital Setting

Zoie A. Aikena1 c1, Michael Wilsona1 and Jonathan Prattena1

a1 Division of Microbial Diseases, UCL Eastman Dental Institute, London, United Kingdom

ATP bioluminescence is being applied in hospitals to measure surface contamination. We compared commercial luminometers for detecting the number Staphylococcus aureus associated with surfaces. The data showed that the ATP bioluminescence methods tested were not robust enough to generate quantitative data on bacterial numbers, especially at low concentrations.

(Received September 08 2010)

(Accepted November 19 2010)

Correspondence

c1 Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WCIX 8LD, United Kingdom, (z.aiken@eastman.ucl.ac.uk)