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Characterization of Satureja khuzestanica Leaf as a Herbal Medicine

Published online by Cambridge University Press:  26 August 2014

Maryam Malmir ,
Rita Serrano ,
Ahmad Reza Gohari  and
Olga Silva
Maryam Malmir
Affiliation:
Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Pharmacological Sciences Group, Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-019 Lisbon, Portugal
Rita Serrano
Affiliation:
Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Pharmacological Sciences Group, Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-019 Lisbon, Portugal
Ahmad Reza Gohari
Affiliation:
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, 16 Azar Avenue, 14155-6451 Tehran, Iran
Olga Silva*
Affiliation:
Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Pharmacological Sciences Group, Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-019 Lisbon, Portugal
*
*Corresponding author. odsilva@ff.ul.pt
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Abstract

Dried leaves of Satureja khuzestanica Jamzad are a popular herbal medicine and dental anesthetic among the nomadic inhabitants of southwestern Iran. The present study establishes criteria for identification of S. khuzestanica dried whole, fragmented and powdered leaves for specification as a herbal substance using macroscopic and microscopic characterization. Quantitative microscopy techniques were also considered. Macroscopically leaves exhibit a grayish-green color, are broadly ovate in shape with an acute apex, attenuate base, and ciliate margin and have a surface covered by an indumentum of glandular and non-glandular trichomes. Microscopically leaves have an isobilateral amphistomatic structure containing peltate glandular trichomes consisting of a multiseriate stalk (five cells) and an enlarged secretory head composed of 12 cells, capitate glandular trichomes of variable morphology together with two types of non-glandular trichomes. Oval-shaped hygromorphic diacytic stomata with an adaxial stomatal index of 13.54, collateral vascular bundles consisting of xylem, and three layers of sclerenchymatous tissue close to phloem together with cluster, prismatic and raphide calcium oxalate crystals were also identified as useful pharmacognostic parameters for identification of S. khuzestanica dried leaves.

Keywords

Satureja khuzestanica leafherbal substancequality controlmacroscopic analysislight microscopyscanning electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 5 , October 2014 , pp. 1425 - 1435
Copyright
© Microscopy Society of America 2014 

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