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Changes in plasma amino acid profiles, growth performance and intestinal antioxidant capacity of piglets following increased consumption of methionine as its hydroxy analogue

Published online by Cambridge University Press:  01 August 2014

Hao Li
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Haifeng Wan
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Yves Mercier
Affiliation:
Adisseo France S.A.S., CERN, Commentry, France
Xiaoling Zhang
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Caimei Wu
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Xiuqun Wu
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Li Tang
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Lianqiang Che
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Yan Lin
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Shengyu Xu
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Gang Tian
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
De Wu
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
Zhengfeng Fang*
Affiliation:
Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya'an625014, People's Republic of China
*
*Corresponding author: Professor Z. Fang, fax +86 28 86290920, email fangzhengfeng@hotmail.com
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Abstract

The aim of the present study was to determine whether early weaning-induced growth retardation could be attenuated by increased consumption of methionine as dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) in both lactating sows and weaned piglets. Therefore, diets containing DLM and HMTBA at 25 % of the total sulphur-containing amino acids (AA) present in the control (CON) diet were fed to lactating sows and weaned piglets and their responses were evaluated. Compared with the CON diet-fed sows, the HMTBA diet-fed sows exhibited a tendency (P< 0·10) towards higher plasma taurine concentrations and the DLM diet-fed sows had higher (P< 0·05) plasma taurine concentrations, but lower (P< 0·05) isoleucine concentrations. Suckling piglets in the HMTBA treatment group had higher (P< 0·05) intestinal reduced glutathione (GSH) content, lower (P< 0·05) oxidised glutathione (GSSG):GSH ratio, and higher (P< 0·05) plasma cysteine and glutathione peroxidase (GPx) activity than those in the CON and DLM treatment groups. The feed intake (P< 0·05) and body weight of piglets averaged across post-weaning (PW) days were higher (P< 0·05) in the HMTBA treatment group than in the DLM treatment group and were higher (P< 0·05) and tended (P< 0·10) to be higher, respectively, in the HMTBA treatment group than in the CON treatment group. Increased (P< 0·05) GSSG content and GSSG:GSH ratio and down-regulated (P< 0·05) expression of nutrient transport genes were observed in the jejunum of piglets on PW day 7 than on PW day 0. On PW day 14, the HMTBA diet-fed piglets had higher (P< 0·05) intestinal GSH content than the CON diet-fed piglets and higher (P< 0·05) plasma GPx activity, villus height and goblet cell numbers than the CON diet- and DLM diet-fed piglets. In conclusion, early weaning-induced growth retardation appears to be attenuated through changes in plasma AA profiles and elevation of growth performance and intestinal antioxidant capacity in piglets following increased consumption of methionine as HMTBA.

Type
Full Papers
Copyright
Copyright © The Authors 2014 

Over the last few years, pigs in the swine industry have largely been weaned at 17·2 d of age, with the average age being 19·3 d( Reference Shankar, Madhusudhan and Harish 1 ). Weaning-induced intestinal dysfunction and growth retardation( Reference Smith, Clark and Overman 2 , Reference Wang, Chen and Li 3 ) have become the major constraints on the practice of improving sow productivity through early weaning. It has been found that gastrointestinal dysfunction induced by early weaning could be attenuated by delayed weaning in pigs( Reference Moeser, Ryan and Nighot 4 ), which indicates the significance of promoting timely growth of neonatal intestine. Given that milk is the main source of nutrition in sucking piglets, maternal nutrition may affect their overall growth and intestinal development via the regulation of milk quantity and quality. Guan et al. ( Reference Guan, Bequette and Ku 5 ) reported that the dietary ratios of true digestible essential amino acids (AA) including methionine:lysine required for milk synthesis in lactating sows should be higher than the values recommended by the National Research Council (NRC)( 6 ). Further studies( Reference Dourmad, Etienne and Valancogne 7 ) have shown that compared with those recommended by the NRC( 6 ), the inclusion levels of methionine and methionine+cysteine should be increased by 15 and 22 %, respectively, in an ideal AA profile for lactating sows. However, no physiological explanation exists for the role of increased methionine consumption in lactating sows and their offspring.

In addition to pre-weaning intestinal development status, the low feed intake is usually considered to be another major factor responsible for intestinal dysfunction and atrophy in weaning piglets( Reference Gu, Li and She 8 ). Previous work has shown gastrointestinal dysfunction to be physiologically associated with early weaning-induced oxidative stress( Reference Wang, Chen and Li 3 ). It has been established that the synthesis of both the major cellular reductant, glutathione( Reference Wu, Fang and Yang 9 ), and the major extracellular reductant, cysteine( Reference Iyer, Ramirez and Ritzenthaler 10 ), depends on the AA cysteine or its precursor, methionine( Reference Nkabyo, Gu and Jones 11 ). Moreover, sulphur-containing amino acids (SAA) have been shown to be essential for normal intestinal mucosal growth in neonatal pigs( Reference Bauchart-Thevret, Stoll and Chang 12 ), and the suppression of intestinal epithelial growth by SAA deficiency has also been demonstrated in neonatal pigs( Reference Bauchart-Thevret, Stoll and Chacko 13 ). These functional roles necessitate a constant nutritional intake of SAA. However, the low feed intake in weaning pigs, especially during the first few post-weaning (PW) days, may decrease the supply of nutritional SAA to levels lower than that practically required.

dl-Methionine (DLM) and its hydroxy analogue, dl-2-hydroxy-4-methylthiobutyrate (HMTBA), are routinely used as methionine supplements in livestock industry. Our previous studies( Reference Fang, Yao and Zhang 14 Reference Fang, Huang and Luo 17 ) in pigs have shown the differences in the absorption and metabolism of these two methionine sources. The objective of the present study was to determine whether early weaning-induced growth retardation could be attenuated by increased consumption of methionine as DLM or HMTBA in both lactating sows and weaned piglets. Plasma and milk AA profiles and intestinal morphology, antioxidant capacity and mRNA abundance of genes related to nutrient transport were also evaluated, which may provide a biological explanation for performance responses.

Materials and methods

Animals and diets

The study protocol was approved by the Animal Care and Use Committee of Animal Nutrition Institute, Sichuan Agricultural University, and the study was carried out in accordance with the NRC Guide for the Care and Use of Laboratory Animals. A total of eighteen pregnant crossed (Landrace × Yorkshire) primiparous sows (with similar body weight and backfat thickness) were used in the experiment. On day 110 of gestation, sows were moved into farrowing crates (2·1 × 0·7 m) with an area (2·1 × 0·6 m) allocated to newborn piglets on each side of the crates in an environmentally regulated farrowing house. Temperature was maintained at 20 ± 1°C in the farrowing house, and heat lamps provided supplemental heat to the pigs. All sows were fed the control (CON) diet until farrowing, after which six sows were continued to be fed the CON diet and the other sows were fed either the DLM or the HMTBA diet (six sows per dietary treatment). The CON diet for lactating sows was formulated based on the NRC( 6 ) lactating sow nutritional requirement values (Table 1). The DLM and HMTBA diets were prepared by adding DLM and HMTBA to the CON diet, respectively, at 25 % of the total SAA present in the CON diet, such that the ratio of methionine+cysteine:lysine approached that recommended by Dourmad et al. ( Reference Dourmad, Etienne and Valancogne 7 ). Within 12 h of farrowing, all litters were standardised to have ten piglets per sow. On postnatal day 14, all litters were standardised to have nine piglets per sow, and piglets were given free access to the diets that they continued to receive after weaning. Piglets from the CON, DLM and HMTBA groups of sows were fed the weaned diets CON, DLM and HMTBA, respectively. The CON diet for piglets was formulated based on the NRC( 6 ) piglet nutritional requirement values (Table 2). The DLM and HMTBA diets were prepared by adding DLM and HMTBA to the CON diet, respectively, at 25 % of the total SAA present in the CON diet. Piglets from three of the six sows in each group were weaned on postnatal days 21 and 28, respectively.

Table 1 Ingredients and composition of the control (CON) diet of sows*

CP, crude protein.

* The dl-methionine (DLM) and dl-2-hydroxy-4-methylthiobutyrate (HMTBA) diets were prepared by adding 1·34 kg of DLM (99 %) and 1·51 kg of HMTBA (88 %) to the CON diet at the expense of maize to supply 25 % of the total sulphur-containing amino acids. Each diet (per kg) contained 14 200 kJ digestible energy.

Provided the following per kg of diet: retinol, 3600 μg; vitamin D3, 70 μg; vitamin E, 100 mg; menadione, 3·5 mg; thiamin, 3·5 mg; riboflavin, 8·5 mg; niacin, 35 mg; d-panthothenic acid, 21 mg; vitamin B6, 3·5 mg; vitamin B12, 35 μg; d-biotin, 420 μg; folic acid, 2·5 mg.

Provided the following per kg of diet: Cu, 10 mg; Fe, 120 mg; Mn, 30 mg; Zn, 80 mg; I, 0·21 mg; Se, 0·23 mg; antioxidant, 100 mg; anti-mould additive, 500 mg.

Table 2 Ingredients and composition of the control (CON) diet of piglets*

CP, crude protein.

* The dl-methionine (DLM) and dl-2-hydroxy-4-methylthiobutyrate (HMTBA) diets were prepared by adding 1·92 kg of DLM (99 %) and 2·16 kg of HMTBA (88 %), respectively, to the CON diet at the expense of maize to supply 25 % of the total sulphur-containing amino acids. Each diet (per kg) contained 14 200 kJ digestible energy.

Provided the following per kg of diet: retinol, 3000 μg; vitamin D3, 60 μg; vitamin E, 60 mg; menadione, 3 mg; thiamin, 3 mg; riboflavin, 7·5 mg; niacin, 30 mg; d-panthothenic acid, 18 mg; vitamin B6, 3 mg; vitamin B12, 30 μg; d-biotin, 360 μg; folic acid, 2·1 mg.

Provided the following per kg of diet: Cu, 200 mg; Fe, 150 mg; Mn, 20 mg; Zn, 150 mg; I, 0·3 mg; Se, 0·3 mg; antioxidant, 100 mg; anti-mould additive, 800 mg.

Collection of blood, milk and tissue samples

At 30 min before the morning meal on postpartum day 21, following an overnight period of feed withdrawal, blood samples (10 ml) were withdrawn from the superior vena cava of each sow into heparinised tubes and immediately centrifuged for 10 min at 2550  g and 4°C. The supernatants were divided into four subsamples and stored at − 20°C until analysis. Milk samples (20 ml) were also collected from each sow on day 21 before the morning meal as described previously( Reference Daza, Riopérez and Centeno 18 ). Briefly, piglets were separated from their dams for 90 min initially, and then 10 international units (IU) of oxytocin (a nonapeptide, 1 IU of oxytocin is the equivalent of about 2 μg of pure peptide) were injected into the ear vein of each sow, and the functional pectoral and inguinal glands were milked manually to collect milk samples, which were stored at − 20°C until analysis.

At 30 min before the morning meal on postnatal days 21, 28 and 35, following an overnight period of feed withdrawal, blood samples were collected from the jugular vein of one pig in each litter as described previously( Reference Yin, Liu and Yin 19 ). Immediately after the collection of blood samples, pigs were anaesthetised and bled by exsanguination as described previously( Reference Fang, Huang and Luo 17 ). A total of fifty-four piglets were slaughtered according to the treatment groups (Table 3). Immediately after slaughter, the abdomen was opened, and the entire intestine was rapidly removed, thoroughly fluxed with sterile saline to remove luminal digesta and then dissected free of mesenteric attachments and placed on a smooth, cold-surface tray. A 2 cm section of tissue samples representing duodenum, jejunum and ileum was collected as described previously( Reference Deng, Yao and Chu 20 ) and stored at − 80°C for subsequent RNA isolation. Samples of intestinal segments were collected for the assessment of intestinal morphology( Reference Wu, Meier and Knabe 21 ). The intestinal segments were opened lengthwise and pinned to a piece of dental wax with the serosal side facing the wax. Subsequently, the samples were fixed in 4 % paraformaldehyde solution with the mucosal side facing downwards to fix the villi vertically( Reference Wu, Ruan and Gao 22 ).

Table 3 Feeding treatments for piglets before slaughter*

CON, control; PW, post-weaning; DLM, dl-methionine; HMTBA, dl-2-hydroxy-4-methylthiobutyrate.

* In CON-21 treatment, piglets were reared for 21 d by sows fed the CON diet; in CON-7PW treatment, piglets were fed the CON diet for seven PW days; and in CON-21+CON-7PW treatment, piglets were reared for 21 d by the CON diet-fed sows and then weaned to be fed the CON diet for seven PW days. The rest of the treatments can be deduced by analogy.

Measurements

Growth performance determination

The body weight of sows was determined before the morning meal on postpartum days 0, 21 and 28. The backfat thickness of sows was measured 65 mm from the left side of the dorsal midline at the last rib level (P2) using ultrasound (Renco Lean-Meater) on postpartum days 0, 21 and 28. The body weight of piglets was determined before the morning meal on postnatal days 0, 21, 28 and 35. The feed intake of each sow and each litter of piglets was recorded daily.

Amino acids analysis

For free AA analysis, the frozen plasma and milk samples were thawed at 4°C. The protein precipitation procedure was carried out as described previously( Reference Kong, Yin and He 23 ). Briefly, 1 ml of the sample and 2·5 ml of 7·5 % (w/v) TCA solution were mixed thoroughly and centrifuged at 12 000  g and 4°C for 15 min. Then, the supernatant was collected and analysed for AA by ion-exchange chromatography using an L8800 high-speed AA analyser (Hitachi) as described by Yin et al. ( Reference Yin, Yin and Li 24 ).

Histological procedure

Intestinal morphology was examined as described in detail by Berkeveld et al. ( Reference Berkeveld, Langendijk and Soede 25 ). From each segment, two transverse tissue sections were cut using a stereo microscope. These sections were dehydrated, embedded together in paraffin wax and sectioned at 5 μm. Later, one section was transferred onto a slide and stained with haematoxylin and eosin. Hence, each slide contained two transverse tissue sections of a gut segment. The height of twelve villi and depth of twelve crypt were determined in each slide using the Nikon Eclipse 80I fluorescence microscope (Nikon) equipped with an epifluorescence image analysis system. Villi and crypts were only measured when there was a complete longitudinal section of a villus and an associated crypt. The average villus height and crypt depth per slide were used as experimental observation values. The number of goblet cells in the intestinal mucosa was determined by periodic acid–Schiff–haematoxylin staining of intestinal sections( Reference Piel, Montagne and Seve 26 ), and it is expressed per 1000 μm length of a villus.

Antioxidant capacity analysis

Frozen jejunal samples were rapidly thawed at 4°C and homogenised on ice in twenty volumes (w/v) of ice-cold physiological saline and centrifuged at 4000  g for 20 min at 4°C, and then the supernatants were collected for reduced glutathione (GSH) and oxidised glutathione (GSSG) analysis. GSH and GSSG contents in the samples were determined using a method described previously( Reference Lora, Alonso and Segura 27 ). Briefly, total glutathione content was determined by calculating the rate of reduction of 5,5′-dithiobis-2-nitrobenzoic acid by GSH at 412 nm and comparing this with a GSH standard curve. GSSG content in the samples was determined using the same method after treating the samples with 4-vinylpyridine for 60 min. GSH content was calculated as follows: total glutathione content – 2 × GSSG content. Protein concentration in jejunal samples was measured using the Bradford method( Reference Bradford 28 ). The activity of glutathione peroxidase (GPx) in plasma was determined by quantifying the rate of H2O2-induced oxidation of GSH to GSSG as described previously( Reference Zhang, Zhu and Cai 29 ). One unit of GPx was defined as the amount required to reduce the level of GSH by 1 μmol/l in 5 min per 0·1 ml of plasma. The activity of GPx in plasma is expressed as units/10 μl.

RNA extraction and real-time PCR

Jejunal tissue samples were used to determine the expression of genes related to the intestinal transport of dietary nutrients. The analysed genes included apo A-IV precursor, FZHUI (fatty acid binding protein human intestine) fatty acid-binding protein, and Na- and Cl-dependent creatine transporter I. Total RNA was extracted from the frozen samples using the RNAiso Plus reagent (Takara Bio, Inc.) according to the manufacturer's specifications. The concentration of RNA in the samples was determined using a DU-800 nucleic and protein detector (Beckman) at an optical density (OD) of 260 nm; an OD260:OD280 ratio ranging between 1·8 and 2·0 was considered acceptable. The integrity of RNA was verified by electrophoresis on a 1 % agarose gel stained with ethidium bromide. Real-time quantitative PCR analysis was carried out using the SYBR Green method and the ABI 7900HT Sequence Detection System. Briefly, first-strand complementary DNA were synthesised from 1 μg of total RNA as described previously( Reference Fang, Luo and Qi 15 ). The thermal cycling parameters were as follows: 95°C for 30 s, followed by forty cycles at 95°C for 15 s and 60°C for 34 s, followed by 95°C for 15 s, 60°C for 1 min and 95°C for 15 s. To confirm specific product amplification, a melting curve analysis was carried out, and the PCR products were also detected by ethidium bromide staining of the 2 % agarose gels after electrophoresis using Tris–acetate–EDTA buffer. Primers for individual genes were designed using Primer Express 3.0 (Applied Biosystems). Primers used in real-time PCR analysis of gene expression in pig jejunum are given in Table 4. The standard curve of each gene was run in duplicate and three times for obtaining reliable amplification efficiency values as described previously( Reference Chen, Chen and Tian 30 ). The correlation coefficients (r) of all the standard curves were >0·99 and the amplification efficiency values were between 90 and 110 %. The relative mRNA abundances of the analysed genes were calculated using the 2-ΔΔC T method( Reference Livak and Schmittgen 31 ), and all the data were normalised to those of the 18S RNA( Reference Petersen, Burrin and Sangild 32 ) in the same samples.

Table 4 Primers used in real-time PCR analysis of gene expression in pig jejunum

Statistical analysis

Data were analysed as described by Littell et al. ( Reference Littell, Henry and Ammerman 33 ). The MIXED procedures of SAS statistical package (version 8.1; SAS Institute, Inc.) were used to analyse repeated-measures data including body weight, feed intake, intestinal morphology, antioxidant capacity and mRNA abundance data. The generalised linear model procedures of SAS statistical package (version 8.1; SAS Institute, Inc.) were used to analyse data such as AA concentrations, which were measured at a single time point. Means of the dietary treatment groups were compared using the least-significant difference test when the F test in the ANOVA was significant. P values < 0·05 were considered statistically significant and P values < 0·10 were considered a tendency towards difference.

Results

Growth performance

There was no difference in the body weight and backfat thickness of sows on postpartum days 0, 21 and 28 (P>0·10) among the dietary treatment groups (Table 5). There was no difference in total feed intake and energy intake per metabolic body weight (P>0·10) among the dietary treatment groups during each of the experimental phases. Piglets weaned at 21 d of age had lower (P< 0·01) day 21–28 weight gain, but higher (P< 0·05) day 28–35 weight gain than those weaned at 28 d of age (Table 6). There was no difference in piglet weight gain (P>0·10) among the dietary treatment groups during these experimental phases. However, it was found that the body weight of piglets averaged over postnatal days was significantly (P< 0·05) higher in the HMTBA treatment group than in the DLM treatment group and tended (P< 0·10) to be higher in the HMTBA treatment group than in the CON treatment group (Fig. 1(A)). With an increase in age, a significant (P< 0·05) increase was observed in the body weight of piglets (Fig. 1(B)). A gradual increase was observed in the feed intake of piglets from PW day 1 to day 14 (Fig. 2(A)). Specifically, feed intake was higher (P< 0·05) on PW day 3 than on PW days 1 and 2, and a further significant (P <0·05) increase was observed on PW days 7 and 10. Overall, feed intake averaged across PW days was higher (P< 0·05) in the HMTBA treatment group than in the CON and DLM treatment groups (Fig. 2(B)).

Table 5 Backfat thickness, body weight and feed intake of sows on postpartum days 0, 21 and 28 (Mean values with their pooled standard errors)

CON, control; DLM, dl-methionine; HMTBA, dl-2-hydroxy-4-methylthiobutyrate.

* Calculated based on daily digestible energy intake as a percentage of metabolic body weight.

Table 6 Comparison of the body weight gain (kg) of piglets weaned at 28 d of age with that of piglets weaned at 21 d of age at each of the experimental phases* (Mean values with their pooled standard errors)

CON, control; DLM, dl-methionine; HMTBA, dl-2-hydroxy-4-methylthiobutyrate.

* Within each of the phases, there were no differences (P>0·10) among the dietary treatment groups.

Fig. 1 Effects of (A) dietary treatment (, control (CON); , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate) and (B) age (, 21 d; , 28 d; ; 35 d) on the body weight of piglets measured over postnatal days. Values are means, with their standard errors represented by vertical bars. a,b,cMean values with unlike letters were significantly different (P< 0·05). * Mean value tended to be different from that of the CON group (P< 0·10).

Fig. 2 Effects of (A) post-weaning (PW) day and (B) dietary treatment (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate) on the feed intake of piglets weaned at 21 d of age. Values are means, with their standard errors represented by vertical bars. a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05).

Plasma free amino acid concentrations of sows

The effects of diets with supplemental DLM or HMTBA on the plasma free AA concentrations of sows on postpartum day 21 are summarised in Table 7. Plasma methionine concentrations showed a tendency (P< 0·10) to be affected by the dietary treatments. Compared with those in the CON diet-fed sows, plasma taurine concentrations tended (P< 0·10) to be higher in the HMTBA diet-fed sows, and higher (P< 0·05) taurine concentrations but lower (P< 0·05) isoleucine concentrations and a tendency (P< 0·10) towards lower lysine concentrations were observed in the DLM diet-fed sows. The DLM diet- and HMTBA diet-fed sows had higher (P< 0·05) plasma free alanine concentrations, but lower (P< 0·05) citrulline concentrations than the CON diet-fed sows.

Table 7 Effects of diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the plasma free amino acid concentrations (μmol/l) of sows on postpartum day 21 (Mean values with their pooled standard errors)

CON, control.

a,bMean values within a row with unlike superscript letters were significantly different (P< 0·05).

* Mean value tended to be different from that of the CON group (P< 0·10).

Milk free amino acid concentrations of sows

The effects of diets with supplemental DLM or HMTBA on the milk free AA concentrations of sows on postpartum day 21 are summarised in Table 8. Milk free leucine and phenylalanine concentrations were higher (P< 0·05) in the DLM diet-fed sows than in the CON diet- and HMTBA diet-fed sows. Compared with the CON diet-fed sows, the HMTBA diet-fed sows had higher (P< 0·05) milk free taurine concentrations and exhibited a tendency (P< 0·10) towards higher valine concentrations, whereas the DLM diet-fed sows had higher (P< 0·05) valine concentrations and exhibited a tendency (P< 0·10) towards higher taurine concentrations. Milk free alanine concentrations were higher (P< 0·05) and histidine concentrations tended (P< 0·10) to be higher in the HMTBA diet-fed sows than in the CON diet-fed sows. The HMTBA diet-fed sows also had higher (P< 0·05) milk free histidine concentrations and exhibited a tendency (P< 0·10) towards higher alanine and ornithine concentrations compared with the DLM diet-fed sows.

Table 8 Effects of diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the milk free amino acid concentrations (μmol/l) of sows on postpartum day 21 (Mean values with their pooled standard errors)

CON, control.

a,bMean values within a row with unlike superscript letters were significantly different (P< 0·05).

* Mean value tended to be different from that of the CON group (P< 0·10).

Plasma free amino acid concentrations of piglets

The effects of sow diets with supplemental DLM or HMTBA on the plasma free AA concentrations of suckling piglets on postnatal day 21 are summarised in Table 9. Plasma free cysteine concentrations were higher (P< 0·05) in the HMTBA treatment group than in the DLM and CON treatment groups. Plasma free citrulline concentrations were higher (P< 0·05) in the CON treatment group than in the DLM and HMTBA treatment groups. Plasma free glutamate concentrations showed a tendency (P< 0·10) to be affected by the dietary treatments.

Table 9 Effects of sow diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the plasma free amino acid concentrations (μmol/l) of suckling piglets on postnatal day 21 (Mean values with their pooled standard errors)

CON, control.

a,bMean values within a row with unlike superscript letters were significantly different (P< 0·05).

Intestinal morphology and goblet cell numbers

The effects of weaning time, dietary treatment, weaning time × dietary treatment interaction, weaning time × intestinal site interaction and dietary treatment × intestinal site interaction on intestinal morphology and goblet cell numbers of piglets are shown in Fig. 3. Representative staining of goblet cells in each group of piglets is shown in Fig. 4. Overall, piglets weaned at 21 d of age had higher (P< 0·01) villus height, lower (P< 0·01) crypt depth and thus higher (P< 0·01) percentage of villus height to crypt depth (PVHCD) than those weaned at 28 d of age (Fig. 3(A)). Dietary treatment also had a significant (P< 0·01) effect on intestinal morphology and goblet cell numbers (Fig. 3(B)). Among the three dietary treatment groups, the HMTBA diet-fed piglets had higher (P< 0·05) villus height and PVHCD than the CON diet- and DLM diet-fed piglets, whereas the CON diet-fed piglets had lower (P< 0·05) goblet cell numbers than the DLM diet- and HMTBA diet-fed piglets (Fig. 3(B)). In Fig. 3(C), the effect of weaning time × dietary treatment interaction on villus height (P< 0·05) and goblet cell numbers (P< 0·01) is shown. The villus height and goblet cell numbers of piglets weaned at 21 d of age were higher (P< 0·05) in the HMTBA treatment group than in the CON and DLM treatment groups, and the goblet cell numbers of piglets weaned at 28 d of age were higher (P< 0·05) in the DLM treatment group than in the CON and HMTBA treatment groups. In Fig. 3(D), the effect of weaning time × intestinal site interaction on villus height (P< 0·01), PVHCD (P< 0·05) and goblet cell numbers (P< 0·01) is shown. Compared with piglets weaned at 21 d of age, those weaned at 28 d of age had lower (P< 0·05) villus height and PVHCD in both the jejunum and ileum, lower (P< 0·05) goblet cell numbers in the duodenum, and higher (P< 0·05) goblet cell numbers in the jejunum. In Fig. 3(E), the effect of dietary treatment × intestinal site interaction on goblet cell numbers is shown (P< 0·01). Both the HMTBA and DLM treatment groups had higher (P< 0·05) goblet cell numbers in the duodenum and ileum than the CON treatment group. In contrast, villus height, crypt depth and PVHCD were not affected (P>0·10) by dietary treatment × intestinal site interaction.

Fig. 3 Effects of (A) weaning time (, 21 d; , 28 d), (B) dietary treatment, (C) weaning time × dietary treatment interaction, (D) weaning time × intestinal site interaction and (E) dietary treatment × intestinal site interaction (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate; , duodenum; , jejunum; , ileum) on the intestinal morphology and goblet cell numbers of piglets on postnatal day 35. Values are means, with their standard errors represented by vertical bars. a,b,c,dMean values with unlike letters were significantly different (P< 0·05). ** Mean values were significantly different between the dietary treatment groups (P< 0·01). PVHCD, percentage of villus height to crypt depth.

Fig. 4 Representative staining (200 × ) of ileal goblet cells in the (A) control, (B) dl-methionine and (C) dl-2-hydroxy-4-methylthiobutyrate diet-fed groups of pigs.

Antioxidant capacity

In Fig. 5(A), the effect (P< 0·01) of dietary treatment on jejunal GSH content, GSSG content and POGRG (percentage of GSSG to GSH) is shown. Compared with the CON treatment group, both the DLM and HMTBA treatment groups had higher (P< 0·05) intestinal GSH and GSSG contents. Moreover, among the three dietary treatment groups, the HMTBA and DLM treatment groups had the highest GSH and GSSG contents, respectively. As a result, POGRG was lower (P< 0·05) in the HMTBA treatment group than in the CON and DLM treatment groups. In Fig. 5(B), the effect of PW day (P< 0·01) on plasma GPx activity and jejunal GSH content, GSSG content and POGRG is shown. Plasma GPx activity was higher (P< 0·05) on PW day 7 than on PW days 0 and 14. Decreased (P< 0·05) intestinal GSH content and increased (P< 0·05) GSSG content were observed on PW day 7 than on PW day 0. Increased (P< 0·05) GSH content and decreased (P< 0·05) GSSG content were observed on PW day 14 than on PW day 7, but neither GSH content nor GSSG content was restored to the level observed on PW day 0. Accordingly, POGRG was higher (P< 0·05) on PW day 14 than on PW day 0, but was lower (P< 0·05) than that observed on PW day 7. Plasma GPx activity (Fig. 5(C)), intestinal GSH content (Fig. 5(D)), GSSG content (Fig. 5(E)) and POGRG (Fig. 5(F)) were affected (P< 0·01) by dietary treatment × PW day interaction. As shown in Fig. 5(C), both the CON and DLM treatment groups had higher (P< 0·05) GPx activity on PW day 7 than on PW days 0 and 14. In contrast, there was little variation in plasma GPx activity in the HMTBA treatment group from PW day 0 to day 14, and it was higher (P< 0·05) than that in the CON and DLM treatment groups on PW days 0 and 14. On PW day 0, intestinal GSH content was highest in the HMTBA treatment group, followed by that in the DLM treatment group, and lowest in the CON treatment group (Fig. 5(D)), intestinal GSSG content was higher (P< 0·05) in the DLM treatment group than in the CON and HMTBA treatment groups (Fig. 5(E)), and POGRG was lower (P< 0·05) in the HMTBA treatment group than in the CON and DLM treatment groups (Fig. 3(F)). The three dietary treatment groups had increased (P< 0·05) GSSG content (Fig. 5(E)) and POGRG (Fig. 5(F)) on PW day 7 than on PW day 0, whereas GSH content (Fig. 5(D)) in the DLM and HMTBA treatment groups was lower (P< 0·05) on PW day 7 than on PW day 0. On PW day 14, both intestinal GSH content (Fig. 5(D)) and GSSG content (Fig. 5(E)) were higher (P< 0·05) in the DLM and HMTBA treatment groups than in the CON treatment group, but POGRG was lower (P< 0·05) in the HMTBA treatment group than in the DLM treatment group (Fig. 5(F)).

Fig. 5 Effects of (A) dietary treatment, (B) post-weaning (PW) day and (C–F) dietary treatment × PW day interaction (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate; , PW day 0; , PW day 7; , PW day 14) on the antioxidant capacity of piglets weaned at 21 d of age. Values are means, with their standard errors represented by vertical bars. a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05). GPx, glutathione peroxidase; GSH, reduced glutathione; GSSG, oxidised glutathione; POGRG, percentage of oxidised glutathione to reduced glutathione.

Gene expression in the jejunum of piglets

In Fig. 6, the effects of weaning time, PW day, dietary treatment and weaning time × PW day interaction on the relative mRNA abundances of genes related to nutrient transport in the jejunum of piglets are shown. Overall, piglets weaned at 21 d of age had higher mRNA abundances of apo A-IV precursor (P< 0·01) and Na- and Cl-dependent creatine transporter I (P< 0·10) than those weaned at 28 d of age (Fig. 6(A)). The mRNA abundances of apo A-IV precursor (P< 0·05), Na- and Cl-dependent creatine transporter I (P< 0·01) and FZHUI fatty acid-binding protein (P< 0·01) were lower on PW day 7 than on PW day 0 (Fig. 6(B)). Dietary treatment also had a significant (P< 0·05) effect on the mRNA abundance of FZHUI fatty acid-binding protein, which was lower (P< 0·05) in the HMTBA treatment group than in the CON and DLM treatment groups (Fig. 6(C)). In Fig. 6(D), the effect (P< 0·05) of weaning time × PW day interaction on the mRNA abundance of apo A-IV precursor is shown; the mRNA abundance of apo A-IV precursor was lower on PW day 7 than on PW day 0 in piglets weaned at 21 d, but no difference was observed between PW days in piglets weaned at 28 d of age.

Fig. 6 Effects of (A) weaning time (, 21 d; , 28 d), (B) post-weaning (PW) day, (C) dietary treatment (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate and (D) weaning time × PW day interaction (, PW day 0; , PW day 7) on the relative mRNA abundances of genes related to nutrient transport in the jejunum of piglets. Values are means, with their standard errors represented by vertical bars. a,bMean values with unlike letters were significantly different (P< 0·05). Mean values were significantly different: * P< 0·05, ** P< 0·01. † Mean values tended to be different between the dietary treatment groups (P< 0·10).

Discussion

The primary aim of the present study was to determine whether early weaning-induced growth retardation could be attenuated by increased consumption of methionine as DLM or its hydroxyl analogue (HMTBA) in both lactating sows and weaned piglets. Given that the lactation performance of sows is, to a great extent, affected by feed intake( Reference Kruse, Traulsen and Krieter 34 ), which is correlated with parity, genotype and body status( Reference Eissen, Kanis and Kemp 35 ), primiparous sows having similar genetic background, body weight and backfat thickness were used in the present experiment. It has also been shown that maternal tissue mobilisation and nutrient requirements during lactation period are affected by litter size( Reference Kim and Easter 36 ). Therefore, all litters were standardised as described previously( Reference Kusina, Pettigrew and Sower 37 ) to have the same number of piglets. Based on the above control, we found no statistical difference in feed intake among the dietary treatment groups during the experimental phases. This may allow us to discuss the difference in lactation performance in association with dietary treatments, specifically methionine levels and sources.

In the present study, the body weight of piglets averaged over postnatal days was found to be higher in the HMTBA treatment group than in the CON and DLM treatment groups. To our knowledge, weaning is always associated with a reduction in food intake in young mammals including piglets( Reference Gu, Li and She 8 , Reference Miller, James and Smith 38 ). The higher feed intake averaged over PW days may account for the faster growth of the HMTBA diet-fed piglets. Compared with age-matched piglets weaned at 28 d of age, piglets weaned at 21 d of age grew slower during postnatal days 21–28, but grew faster during postnatal days 28–35, which is in good agreement with the results of previous studies( Reference Wang, Chen and Li 3 , Reference Colson, Orgeur and Foury 39 ). These observations indicate the negative effect of weaning on piglet growth, especially during the first few PW days. In addition, on postnatal day 35, piglets weaned at 28 d of age tended to weigh more than those weaned at 21 d of age in the DLM treatment group; however, the body weight of piglets on postnatal day 35 in the HMTBA treatment group was not different between the weaning ages. These results indicate that increased consumption of methionine as HMTBA might alleviate stresses associated with early weaning.

The secondary aim of the present study was to investigate the mechanisms underlying the responses of lactating sows and their offspring to increased consumption of methionine sources. An important finding was that plasma and milk AA profiles were responsive to the increased consumption of methionine as DLM or HMTBA. The DLM diet-fed sows had lower plasma lysine and isoleucine concentrations than the CON diet- and HMTBA diet-fed sows. The decreased lysine concentrations may be associated with the fact that lysine and methionine share the B0,+ and b0,+ transport systems in mammalian intestine( Reference Broer 40 ). In support of this view, Hagihira et al. ( Reference Hagihira, Lin and Samiy 41 ) first demonstrated mutual inhibition between arginine, cystine, lysine and ornithine and observed some inhibition by methionine in the intestinal epithelium. A down-regulation effect of l-methionine on these transporters has also been reported for broiler chickens as a means to lower the risk of methionine intoxication( Reference Soriano-Garcia, Torras-Llort and Moreto 42 ). Lysine is often considered to be the first limiting AA for lactating sows sustaining milk synthesis, particularly when diets are based on maize and soyabean meal as the main protein sources( 6 ). Recent studies have indicated that isoleucine plays an important role in the activation of mammalian target of rapamycin signalling, which is a control point in milk protein synthesis( Reference Appuhamy, Knoebel and Nayananjalie 43 ). It appears that the lower concentrations of isoleucine and lysine in the plasma of the DLM diet-fed sows may limit these two AA available for delivery to the mammary gland and thus limit milk synthesis. This may be further supported by the observation that the higher free leucine, valine, phenylalanine and tyrosine concentrations in the milk of the DLM diet-fed sows did not cause a difference in the body weight of 21-d-old piglets among the dietary treatment groups. There is evidence that intestinal mucosal catabolism of branched-chain AA may provide nitrogen for the synthesis of both alanine and glutamate( Reference Chen, Li and Wang 44 ). It has also been observed that the extensive catabolism of branched-chain AA in extra-intestinal tissues stimulates the synthesis of glutamate and glutamine( Reference Wu, Thompson and Sedgwick 45 , Reference Li, Knabe and Kim 46 ). It appears that the higher concentrations of leucine and valine in the milk of the DLM diet-fed sows might explain the potential change in piglet plasma glutamate concentrations, which exhibited a tendency to be affected by the dietary treatments.

The concentrations of non-essential AA including taurine, citrulline and alanine in plasma and milk were also affected by increased consumption of methionine as DLM or HMTBA. Given that taurine is an end product of methionine metabolism, the higher taurine concentrations in the plasma and milk of the DLM diet- and HMTBA diet-fed sows than in those of the CON diet-fed sows might be due to the increased availability of methionine, which showed a tendency to be affected by the dietary treatments. Increased alanine concentrations and decreased citrulline concentrations in plasma were also observed following DLM and HMTBA supplementation compared with those in the CON diet-fed sows. In a previous study, the uptake of glutamine in vivo and the release of citrulline by the small intestine have been observed in pigs( Reference Wu, Borbolla and Knabe 47 ). In this regard, the higher concentrations of citrulline in plasma indicate that more amounts of glutamine were catabolised in the intestine of the CON diet-fed sows. Branched-chain AA catabolism has been shown to be an important rate-limiting event in alanine production in vivo ( Reference Haymond, Ben-Galim and Strobel 48 ). The decreased plasma isolecuine concentrations appear to be suggestive of more extensive catabolism, which may account for the increased alanine production in the DLM diet-fed sows.

The intestine is considered to be most susceptible to weaning stresses( Reference Gu, Li and She 8 , Reference Miller, James and Smith 38 ). Moreover, gastrointestinal dysfunction has been shown to be physiologically strongly associated with early weaning-induced oxidative stress( Reference Wang, Chen and Li 3 ). Thus, the antioxidant capacity was evaluated to determine the mechanisms that account for the differences among piglets consuming different levels and sources of methionine. Weaning was found to lead to elevated plasma GPx activity and intestinal GSSG:GSH ratio in piglets in the CON and DLM treatment groups, which is in good agreement with the results of recent studies( Reference Degroote, Michiels and Claeys 49 ). It has been established that GPx and glutathione play an important role in the maintenance of redox balance of cells through the elimination of reactive oxygen species such as H2O2 ( Reference Trachootham, Lu and Ogasawara 50 ), which can be converted into oxygen through coupled reactions with the conversion of GSH into GSSG, catalysed by GPx. The GSSG:GSH ratio has been shown to be a good measure of oxidative stress of an organism( Reference Dröge 51 ). The increased GPx activity accompanied by an increased GSSG:GSH ratio, as has been observed in the present study and previous studies( Reference Degroote, Michiels and Claeys 49 ), indicates that the variation in GPx activity is an adaptive response to oxidative stress associated with early weaning. Interestingly, increased consumption of methionine as HMTBA in lactating sows and weaned piglets resulted in relatively constant plasma GPx activity in piglets from PW day 0 to day 14. One possible explanation is that the increased plasma cysteine concentrations may enhance the capacity of reactive oxygen species elimination. In support of this view, cysteine is particularly sensitive to reactive oxygen species and thus is a quantitatively important reactive oxygen species scavenger( Reference Stadtman 52 ). Given that the synthesis of both the major cellular reductant, glutathione( Reference Wu, Fang and Yang 9 ), and the major extracellular reductant, cysteine( Reference Iyer, Ramirez and Ritzenthaler 10 ), depends on the AA cysteine or its precursor, methionine( Reference Nkabyo, Gu and Jones 11 ), the higher intestinal GSH content and plasma cysteine concentrations at weaning and the lower GSSG:GSH ratio as observed at weaning and PW day 14 may reflect the high efficiency of methionine source as HMTBA than as DLM to enhance the antioxidant capacity of piglets. In addition, consistent with previous studies( Reference Wang, Chen and Li 3 ), it was found that the expression of intestinal transport genes including apo A-IV precursor, FZHUI fatty acid-binding protein and Na- and Cl-dependent creatine transporter I in the jejunum of piglets was lower on PW day 7 than on PW day 0, indicating the significant role of weaning in the reduction of the expression of these transport genes. Interestingly, the expression of FZHUI fatty acid-binding protein was also affected by the dietary treatments with a down-regulated expression being observed in the HMTBA treatment group, the underlying mechanism of which remains to be elucidated. Compared with the CON treatment group, higher villus height and villus height:crypt depth ratio and higher number of goblet cells were also observed in the intestine of piglets in the HMTBA treatment group, which provides further evidence for the alleviated weaning stresses following HMTBA consumption.

In summary, increased consumption of methionine as HMTBA was found to alleviate early weaning-induced growth retardation in piglets, which is physiologically associated with elevated amounts of SAA available for delivery to extra-intestinal tissues without compromising lysine and isoleucine availability, and promote growth performance and antioxidant capacity of the intestine. This novel finding may have important implications for the nutritional management of lactating sows and weaning piglets.

Acknowledgements

The authors thank Professor Mei Yu at Huazhong Agricultural University for her valuable assistance in statistical analysis.

The present study was supported by the Rhodimet Research Grant from Adisseo France S.A.S., Briand, Antony Cedex, France, the National Natural Science Foundation of China (30901042), and the Sichuan Province Science Foundation for Fostering Youths Talents (2011JQ0015).

The authors' contributions are as follows: Z. F. designed the study; H. L., H. W., X. Z., C. W., X. W., L. T., L. C., Y. L., S. X. and G. T. carried out the study; Z. F., Y. M., D. W. and H. L. analysed the data and wrote the article.

The authors declare potential conflict of interest, given that Y. M. is an employee of Adisseo, one of financial supporters of the present study.

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Figure 0

Table 1 Ingredients and composition of the control (CON) diet of sows*

Figure 1

Table 2 Ingredients and composition of the control (CON) diet of piglets*

Figure 2

Table 3 Feeding treatments for piglets before slaughter*

Figure 3

Table 4 Primers used in real-time PCR analysis of gene expression in pig jejunum

Figure 4

Table 5 Backfat thickness, body weight and feed intake of sows on postpartum days 0, 21 and 28 (Mean values with their pooled standard errors)

Figure 5

Table 6 Comparison of the body weight gain (kg) of piglets weaned at 28 d of age with that of piglets weaned at 21 d of age at each of the experimental phases* (Mean values with their pooled standard errors)

Figure 6

Fig. 1 Effects of (A) dietary treatment (, control (CON); , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate) and (B) age (, 21 d; , 28 d; ; 35 d) on the body weight of piglets measured over postnatal days. Values are means, with their standard errors represented by vertical bars. a,b,cMean values with unlike letters were significantly different (P< 0·05). * Mean value tended to be different from that of the CON group (P< 0·10).

Figure 7

Fig. 2 Effects of (A) post-weaning (PW) day and (B) dietary treatment (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate) on the feed intake of piglets weaned at 21 d of age. Values are means, with their standard errors represented by vertical bars. a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05).

Figure 8

Table 7 Effects of diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the plasma free amino acid concentrations (μmol/l) of sows on postpartum day 21 (Mean values with their pooled standard errors)

Figure 9

Table 8 Effects of diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the milk free amino acid concentrations (μmol/l) of sows on postpartum day 21 (Mean values with their pooled standard errors)

Figure 10

Table 9 Effects of sow diets with supplemental dl-methionine (DLM) or dl-2-hydroxy-4-methylthiobutyrate (HMTBA) on the plasma free amino acid concentrations (μmol/l) of suckling piglets on postnatal day 21 (Mean values with their pooled standard errors)

Figure 11

Fig. 3 Effects of (A) weaning time (, 21 d; , 28 d), (B) dietary treatment, (C) weaning time × dietary treatment interaction, (D) weaning time × intestinal site interaction and (E) dietary treatment × intestinal site interaction (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate; , duodenum; , jejunum; , ileum) on the intestinal morphology and goblet cell numbers of piglets on postnatal day 35. Values are means, with their standard errors represented by vertical bars. a,b,c,dMean values with unlike letters were significantly different (P< 0·05). ** Mean values were significantly different between the dietary treatment groups (P< 0·01). PVHCD, percentage of villus height to crypt depth.

Figure 12

Fig. 4 Representative staining (200 × ) of ileal goblet cells in the (A) control, (B) dl-methionine and (C) dl-2-hydroxy-4-methylthiobutyrate diet-fed groups of pigs.

Figure 13

Fig. 5 Effects of (A) dietary treatment, (B) post-weaning (PW) day and (C–F) dietary treatment × PW day interaction (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate; , PW day 0; , PW day 7; , PW day 14) on the antioxidant capacity of piglets weaned at 21 d of age. Values are means, with their standard errors represented by vertical bars. a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05). GPx, glutathione peroxidase; GSH, reduced glutathione; GSSG, oxidised glutathione; POGRG, percentage of oxidised glutathione to reduced glutathione.

Figure 14

Fig. 6 Effects of (A) weaning time (, 21 d; , 28 d), (B) post-weaning (PW) day, (C) dietary treatment (, control; , dl-methionine; , dl-2-hydroxy-4-methylthiobutyrate and (D) weaning time × PW day interaction (, PW day 0; , PW day 7) on the relative mRNA abundances of genes related to nutrient transport in the jejunum of piglets. Values are means, with their standard errors represented by vertical bars. a,bMean values with unlike letters were significantly different (P< 0·05). Mean values were significantly different: * P< 0·05, ** P< 0·01. † Mean values tended to be different between the dietary treatment groups (P< 0·10).