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Quantifying plant cell-wall failure in vivo using nanoindentation

Published online by Cambridge University Press:  28 August 2014

Elham Forouzesh ,
Ashwani K. Goel  and
Joseph A. Turner
Elham Forouzesh
Affiliation:
Mechanical and Materials Engineering, University of Nebraska-Lincoln, W342 Nebraska Hall, Lincoln, Nebraska 68588-0526
Ashwani K. Goel
Affiliation:
Mechanical and Materials Engineering, University of Nebraska-Lincoln, W342 Nebraska Hall, Lincoln, Nebraska 68588-0526
Joseph A. Turner*
Affiliation:
Mechanical and Materials Engineering, University of Nebraska-Lincoln, W342 Nebraska Hall, Lincoln, Nebraska 68588-0526
*
Address all correspondence to Joseph A. Turner atjaturner@unl.edu
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Abstract

Nanoindentation experiments have been carried on Arabidopsis thaliana using spherical tungsten tips. Load–displacement plots obtained from experiments suggest that there is an optimum diameter of tip size which can be used to safely penetrate the tip through the cell wall. Based on the exact tip size used in the experiments and the measured load–displacement response, the failure stress was calculated using the experimental data in conjunction with a computational model. The value of failure stress was investigated in hypertonic (plasmolyzed), isotonic, and hypotonic (turgid) samples.

Type
Research Letters
Information
MRS Communications , Volume 4 , Issue 3 , September 2014 , pp. 107 - 111
Copyright
Copyright © Materials Research Society 2014 

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