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Whole Cell Cryo-Electron Tomography Suggests Mitochondria Divide by Budding

Published online by Cambridge University Press:  28 May 2014

Guo-Bin Hu
Guo-Bin Hu*
Affiliation:
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
*
*Corresponding author. ghu@crystal.harvard.edu
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Abstract

Eukaryotes rely on mitochondrial division to guarantee that each new generation of cells acquires an adequate number of mitochondria. Mitochondrial division has long been thought to occur by binary fission and, more recently, evidence has supported the idea that binary fission is mediated by dynamin-related protein (Drp1) and the endoplasmic reticulum. However, studies to date have depended on fluorescence microscopy and conventional electron microscopy. Here, we utilize whole cell cryo-electron tomography to visualize mitochondrial division in frozen hydrated intact HeLa cells. We observe a large number of relatively small mitochondria protruding from and connected to large mitochondria or mitochondrial networks. Therefore, this study provides evidence that mitochondria divide by budding.

Keywords

cryo-EMcryo-electron tomographymitochondrionmitochondrial divisionmitochondrial buddingmitochondrial dynamics
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 4 , August 2014 , pp. 1180 - 1187
Copyright
© Microscopy Society of America 2014 

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Hu Supplementary Material

Movie S1, a round-trip slice-wise show of the 3D map associated with Fig. 2A and D.

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Video 9 MB

Hu Supplementary Material

Movie S2, a round-trip slice-wise display of the 3D map associated with Fig. 2B and E.

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Video 10.3 MB

Hu Supplementary Material

Movie S3, a round-trip slice-wise display of the 3D map associated with Fig. 2C and F.

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Video 6.4 MB
Supplementary material: File

Hu Supplementary Material

Figures S1-S4

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