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A SSR kit to study genetic diversity in chickpea (Cicer arietinum L.)

Published online by Cambridge University Press:  16 July 2014

Rajeev Varshney ,
Mahendar Thudi ,
Hari Upadhyaya ,
Sangam Dwivedi ,
Sripada Udupa ,
Bonnie Furman ,
Michael Baum  and
David Hoisington
Rajeev Varshney*
Affiliation:
Research Program Grain Legumes, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India
Mahendar Thudi
Affiliation:
Research Program Grain Legumes, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India
Hari Upadhyaya
Affiliation:
Research Program Grain Legumes, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India
Sangam Dwivedi
Affiliation:
Research Program Grain Legumes, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India
Sripada Udupa
Affiliation:
International Center for Agricultural Research in the Dry Areas, Rabat, Morocco
Bonnie Furman
Affiliation:
International Center for Agricultural Research in the Dry Areas, Rabat, Morocco
Michael Baum
Affiliation:
International Center for Agricultural Research in the Dry Areas, Amman, Jordan
David Hoisington
Affiliation:
Research Program Grain Legumes, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India The University of Georgia, Athens, GA, USA
*
* Corresponding author. E-mail: r.k.varshney@cgiar.org
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Abstract

A chickpea simple sequence repeat (SSR) marker reference kit has been developed based on the genotyping of the global chickpea composite collection (3,000 accessions) with 35 SSR markers. The kit consists of three pools of chickpea accessions along with supporting documentation on the SSR markers, polymerase chain reaction and detection conditions, and the expected allele sizes for each of the 35 SSR loci. These markers were selected based on quality criteria, genome coverage and locus-specific information content. Other important SSR selection criteria were quality of amplification products, locus complexity, polymorphism information content and well-dispersed location on a chickpea genetic map. The developed SSR kit has a wide range of applications, especially for genetic diversity studies in chickpea. Using the markers and reference accessions in the kit, scientists in other laboratories will be able to compare the genotypic data that they obtain for their germplasm with that obtained using the global composite collection.

Keywords

allele sizescomposite collectionpolymorphism information contentsimple sequence repeats
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S118 - S120
Copyright
Copyright © NIAB 2014 

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References

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Supplementary material: File

Varshney Supplementary Material

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