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Integrated Visible optical filter and photodetector for detection of FRET signals

Published online by Cambridge University Press:  10 June 2014

P. Louro ,
A. Charneca ,
V. Silva ,
M. Vieira  and
A. Karmali
P. Louro
Affiliation:
Electronics Telecommunications and Computer Dept, ISEL, Lisbon, Portugal. CTS-UNINOVA, Lisbon, Portugal.
A. Charneca
Affiliation:
CIEB-ISEL, R. Conselheiro Emidio Navarro, 1, Lisbon, Portugal
V. Silva
Affiliation:
Electronics Telecommunications and Computer Dept, ISEL, Lisbon, Portugal.
M. Vieira
Affiliation:
Electronics Telecommunications and Computer Dept, ISEL, Lisbon, Portugal. CTS-UNINOVA, Lisbon, Portugal. DEE-FCT-UNL, Quinta da Torre, Monte da Caparica, 2829-516, Caparica, Portugal
A. Karmali
Affiliation:
CIEB-ISEL, R. Conselheiro Emidio Navarro, 1, Lisbon, Portugal
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Abstract

In this paper we present a multilayer device based on a-Si:H/a-SiC:H that operates as photodetector and optical filter. The use of such device in protein detection applications is pertinent in Fluorescence Resonance Energy Transfer (FRET) measurements that demand the detection of visible fluorescent signals located at specific wavelengths bands. This device was designed to operate in the visible range with a selective sensitivity dependent on the applied electrical bias. Several nanosensors were tested with a commercial spectrophotometer to judge the performance of the FRET signals using glucose solutions of different concentrations. Two nanosensors (FLIPglu-90μM and FLIPglu-600μM) were tested with a commercial spectrofluorimeter to judge the performance of the FRET signals by using glucose solutions of different concentrations. These measurements were carried out by using these nanosensors both in the free form and immobilized form on inner epidermis of onion bulb scale. The proposed device was used to demonstrate the possibility of FRET signals detection, using visible signals of similar wavelength and intensity. The device sensitivity was tuned to enhance the wavelength band of interest using adequate electrical biasing.

Keywords

sensorsemiconductingSi
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1689: Symposium Z – Bioelectronics—Materials, Processes and Applications , 2014 , mrss14-1689-z07-11
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

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