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Microsatellite variation of mussels (Mytilus galloprovincialis) in central and eastern Mediterranean: genetic panmixia in the Aegean and the Ionian Seas

Published online by Cambridge University Press:  11 March 2014

Ioannis A. Giantsis ,
Nadia Mucci ,
Ettore Randi ,
Theodore J. Abatzopoulos  and
Apostolos P. Apostolidis
Ioannis A. Giantsis
Affiliation:
Laboratory of Ichthyology and Fisheries, Department of Animal Production, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
Nadia Mucci
Affiliation:
Laboratorio di Genetica, Istituto Superiore per la Protezione e la Ricerca Ambientale (ISPRA), Via Ca Fornacetta 9, I -40064 Ozzano dell'Emilia (Bologna), Italy
Ettore Randi
Affiliation:
Laboratorio di Genetica, Istituto Superiore per la Protezione e la Ricerca Ambientale (ISPRA), Via Ca Fornacetta 9, I -40064 Ozzano dell'Emilia (Bologna), Italy
Theodore J. Abatzopoulos
Affiliation:
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece
Apostolos P. Apostolidis*
Affiliation:
Laboratory of Ichthyology and Fisheries, Department of Animal Production, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
*
Correspondence should be addressed to: A.P. Apostolidis, Laboratory of Ichthyology and Fisheries, Department of Animal Production, Faculty of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece email: apaposto@agro.auth.gr
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Abstract

Genetic variation of mussels Mytilus galloprovincialis in central–eastern Mediterranean Sea is investigated in this study. A total of 550 individuals sampled from two cultured and 11 wild populations from Italy, Croatia, Greece and Turkey were genotyped at 10 microsatellite loci. Significant deviations from Hardy–Weinberg expectations were observed in more than 75% of the tests performed. All populations showed extensive heterozygote deficits, which remained at significant levels even after correction for null alleles, providing evidence that null alleles were only partly responsible for deviations from Hardy–Weinberg equilibrium in these molluscs. Moreover, null alleles seem to have limited influence on the population genetic differentiation. Similar levels of multi-locus heterozygosity and allelic richness were observed in all populations, cultured and wild, implying the sustainability of the exploited populations. Lack of isolation by distance and markedly low genetic differentiation between the nine Greek sampling sites (shoreline >1000 km) was revealed by Mantel tests, FST values, exact tests and analyses of molecular variance, indicating that mussels from these regions are either at or close to panmixia. Similarly, patterns of genetic homogeneity were also found between the two Italian samples, whereas the observed genetic differentiation of the populations from Turkey and Croatia probably reflects the specific topographic and oceanographic conditions of these regions.

Keywords

genetic structureheterozygote deficiencymicrosatellitesMediterraneanMytilus galloprovincialispanmixia
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 94 , Issue 4 , June 2014 , pp. 797 - 809
Copyright
Copyright © Marine Biological Association of the United Kingdom 2014 

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