British Journal of Nutrition

Research Article

Lycopene supplementation attenuated xanthine oxidase and myeloperoxidase activities in skeletal muscle tissues of rats after exhaustive exercise

Chieh-Chung Liua1, Chi-Chang Huanga2, Wan-Teng Lina3a4, Chin-Cheng Hsieha5, Shih-Yi Huanga2, Su-Jiun Lina6 and Suh-Ching Yanga2 c1

a1 Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan

a2 School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan

a3 Department of Nutrition and Food Sciences, Fu-Jen Catholic University, Taipei, Taiwan

a4 De Lin Institute of Technology, Taipei, Taiwan

a5 Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan

a6 Graduate Institute of Biology and Environment Science, School of Cellular and Molecular Biology, University of New Haven, CT 06516, USA

Abstract

Strenuous exercise is known to induce oxidative stress leading to the generation of free radicals. The purpose of the present study was to investigate the effects of lycopene, an antioxidant nutrient, at a relatively low dose (2·6 mg/kg per d) and a relatively high dose (7·8 mg/kg per d) on the antioxidant status of blood and skeletal muscle tissues in rats after exhaustive exercise. Rats were divided into six groups: sedentary control (C); sedentary control with low-dose lycopene (CLL); sedentary control with high-dose lycopene (CHL); exhaustive exercise (E); exhaustive exercise with low-dose lycopene (ELL); exhaustive exercise with high-dose lycopene (EHL). After 30 d, the rats in the three C groups were killed without exercise, but the rats in the three E groups were killed immediately after an exhaustive running test on a motorised treadmill. The results showed that xanthine oxidase (XO) activities of plasma and muscle, and muscular myeloperoxidase (MPO) activity in group E were significantly increased compared with group C. Compared with group E, the elevations of XO and MPO activities of muscle were significantly decreased in group EHL. The malondialdehyde concentrations of plasma and tissues in group E were significantly increased by 72 and 114 %, respectively, compared with those in group C. However, this phenomenon was prevented in rats of the ELL and EHL groups. There was no significant difference in the GSH concentrations of erythrocytes in each group; however, exhaustive exercise resulted in a significant decrease in the GSH content of muscle. In conclusion, these results suggested that lycopene protected muscle tissue from oxidative stress after exhaustive exercise.

(Received March 10 2005)

(Revised June 01 2005)

(Accepted June 07 2005)

Correspondence:

c1 *Corresponding author: Dr Suh-Ching Yang, fax +886 2 2737 3112, email sokei@tmu.edu.tw