British Journal of Nutrition

Research Article

Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids

I. Wąsowskaa1a2, M. R. G. Maiaa1a3, K. M. Niedźwiedzkaa1a2, M. Czaudernaa2, J. M. C. Ramalho Ribeiroa3, E. Devillarda1, K. J. Shingfielda4 and R. J. Wallacea1 c1

a1 Rowett Research Institute Greenburn Road Bucksburn Aberdeen AB21 9SB UK

a2 Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences 05-110 Jablonna Poland

a3 Estaça˜o Zootécnica Nacional Fonte Boa 2005-048 Vale de Santarém Portugal

a4 Animal Production ResearchMTT Agrifood Research Finland 31600 Jokioinen Finland

Abstract

Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or α-linolenic acid (LNA) was incubated (1·67g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay–concentrate ration. Adding fish oil (up to 4·17g/l) tended to decrease the initial rate of LA (P=0·025) and LNA (P=0·137) disappearance, decreased (P<0·05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P<0·05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5 n-3) or DHA (22:6 n-3), the major fatty acids in fish oil, were low (100mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.

(Received July 08 2005)

(Revised February 06 2006)

(Accepted February 10 2006)

Correspondence:

c1 *Corresponding author: Dr R. John Wallace, fax +44 1224 716687, email rjw@rowett.ac.uk

0Comments