British Journal of Nutrition

Research Article

The influence of moderate red wine consumption on antioxidant status and indices of oxidative stress associated with CHD in healthy volunteers

Catherine Tsanga1, Siobhan Higginsa2, Garry G. Duthiea3, Susan J. Duthiea3, Moira Howiea4 p1, William Mullena1, Michael E. J. Leana5 and Alan Croziera1 c1

a1 Plant Products and Human Nutrition Group, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK

a2 Department of Human Nutrition, University of Glasgow, Yorkhill Hospitals, Glasgow G3 8SJ, UK

a3 Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK

a4 Safeway Stores plc, 6 Millington Road, Hayes, Middlesex, UB3 4AY, UK

a5 Department of Human Nutrition, University of Glasgow, Queen Elizabeth Building, Royal Infirmary, Glasgow, G23 2ER, UK

Abstract

The effects of moderate red wine consumption on the antioxidant status and indices of lipid peroxidation and oxidative stress associated with CHD were investigated. A randomised, controlled study was performed with twenty free-living healthy volunteers. Subjects in the red wine group consumed 375 ml red wine daily for 2 weeks. We measured the total concentration of phenolics and analysed the individual phenolics in the wine and plasma by HPLC with tandem MS. The antioxidant capacity of plasma was measured with electron spin resonance spectroscopy while homocysteine and fasting plasma lipids were also determined. The production of conjugated dienes and thiobarbituric acid-reactive substances (TBARS) were measured in Cu-oxidised LDL. Plasma total phenolic concentrations increased significantly after 2 weeks of daily red wine consumption (P≤0·001) and trace levels of metabolites, mainly glucuronides and methyl glucuronides of (+)-catechin and (−)-epicatechin, were detected in the plasma of the red wine group. These flavan-3-ol metabolites were not detected in plasma from the control group. The maximum concentrations of conjugated dienes and TBARS in Cu-oxidised LDL were reduced (P≤0·05) and HDL cholesterol concentrations increased (P≤0·05) following red wine consumption. The findings from the present study provide some evidence for potential protective effects of moderate consumption of red wine in healthy volunteers.

(Received April 20 2004)

(Revised September 24 2004)

(Accepted September 26 2004)

Correspondence:

c1 Corresponding author: Professor A. Crozier, fax +44 141 330 5394, email a.crozier@bio.gla.ac.uk

p1 Waitrose, Bracknell, Berkshire, RG12 8YA, UK.

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