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Molecular genetic conspecificity of Spiculopteragia houdemeri (Schwartz, 1926) and S. andreevae (Dróżdż, 1965) (Nematoda: Ostertagiinae) from wild ruminants in Japan

Published online by Cambridge University Press:  16 October 2012

K. Sultan ,
M. Omar ,
P. Makouloutou ,
Y. Kaneshiro ,
E. Saita ,
M. Yokoyama ,
K. Suzuki ,
E. Hosoi  and
H. Sato
K. Sultan
Affiliation:
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
M. Omar
Affiliation:
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
P. Makouloutou
Affiliation:
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
Y. Kaneshiro
Affiliation:
NPO Shikoku Institute of Natural History, 470-1 Shimobu-otsu, Susaki, Kochi785-0023, Japan
E. Saita
Affiliation:
Wildlife Management Research Center, Hyogo, 940 Sawano, Aogaki-cho, Tanba, Hyogo669-3842, Japan
M. Yokoyama
Affiliation:
Wildlife Management Research Center, Hyogo, 940 Sawano, Aogaki-cho, Tanba, Hyogo669-3842, Japan Nature and Environment Division, Institute of Natural and Environmental Sciences, University of Hyogo, 940 Sawano, Aogaki-cho, Tanba, Hyogo669-3842, Japan
K. Suzuki
Affiliation:
Hikiiwa Park Center, 1629 Inari-cho, Tanabe646-0051, Japan
E. Hosoi
Affiliation:
Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
H. Sato*
Affiliation:
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi753-8515, Japan
*
*Fax: +81-83-933-5902 E-mail: sato7dp4@yamaguchi-u.ac.jp
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Abstract

Male dimorphism of the subfamily Ostertagiinae (Nematoda: Trichostrongylidae) is a well-known phenomenon, and two or more morphotypes of a single species have previously been described as different species. Two Spiculopteragia spp., S. houdemeri (syn. S. yamashitai) and S. andreevae (syn. Rinadia andreevae) recorded in Asian cervids and wild bovids, are considered to represent major and minor morphs of S. houdemeri, respectively, based solely on their co-occurrence in the same host individual along with monomorphic females. In this study, males of morph houdemeri ( = S. houdemeri) and morph andreevae ( = S. andreevae) as well as females with three different vulval ornamentations were collected from sika deer (Cervus nippon) and Japanese serows (Capricornis crispus) distributed on the mainland of Japan. Morphologically characterized worms were subjected to molecular genetic analyses based on the internal transcribed spacer region of the ribosomal RNA gene and a partial region of the cytochrome c oxidase subunit I gene of mitochondrial DNA. Of 181 collected sika deer, 177 (97.8%) and 73 (40.3%) deer harboured males of morphs houdemeri and andreevae, respectively. Worm numbers of the former morph were found to range between 1 and 444 per individual, whereas only 1–25 worms per individual were detected for the latter morph. Five out of six serows harboured 47–71 or 2–9 males of morphs houdemeri and andreevae per individual, respectively. Females with one or two vulval flaps were predominant, but there was a substantial presence of flapless females in both host species. All the morphs of male and female adults had an identical genetic background, thus directly confirming the morphological polymorphism of S. houdemeri.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 1 , March 2014 , pp. 1 - 12
Copyright
Copyright © Cambridge University Press 2012 

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