British Journal of Nutrition

Review Article

Background diet influences the anti-inflammatory effect of α-linolenic acid in dyslipidaemic subjects

George K. Paschosa1, Loukianos S. Rallidisa2, Georgios K. Liakosa3, Demosthenes Panagiotakosa1, George Anastasiadisa4, Vasilios Votteasa4 and Antonis Zampelasa1 c1

a1 Department of Nutrition and Dietetics, Harokopio University, 70 El Venizelou Street, Athens 17671, Greece

a2 Second Department of Cardiology, General Hospital of Nikea, Piraeus, Greece

a3 Biochemistry Laboratory, General Hospital of Nikea, Piraeus, Greece

a4 Department of Cardiology, Laiko Hospital, Athens, Greece


Long-chain n-3 PUFA from fish oils are known to have anti-inflammatory effects. We evaluated the effect of α-linolenic acid (ALA), precursor of n-3 fatty acids, on serum inflammatory markers and soluble cellular adhesion molecules (sCAM) of dyslipidaemic males, relative to their background diet. Participants were assigned to two groups, based upon food intake patterns: (a) twenty-one dyslipidaemic subjects who habitually ate a Mediterranean–Cretan-type diet; (b) nineteen dyslipidaemic subjects who normally ate a Westernised Greek diet. All were supplemented with 8·1 g ALA/d for 12 weeks. We determined serum amyloid A (SAA), C-reactive protein (CRP), macrophage colony-stimulating factor (MCSF), IL-6, soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular adhesion molecule-1 and soluble E-selectin concentrations at the beginning and the end of the ALA supplementation period. Serum baseline concentrations of inflammatory markers and sCAM were similar across the diet groups. Type of diet had a significant impact on the response of inflammatory markers to ALA supplementation. The Westernised Greek diet group showed a reduction in SAA (P<0·001), CRP (P=0·002), MCSF (P=0·005) and IL-6 (P=0·04) concentrations. The Mediterranean–Cretan-type background diet group showed a significant reduction only in MCSF concentrations (P=0·003). The sVCAM-1 concentrations were significantly reduced in both the Westernised Greek diet group (P=0·001) and the Mediterranean–Cretan-type diet group (P<0·001). The present study demonstrated that ALA supplementation lowered the serum concentrations of inflammatory markers more profoundly when the background diet was rich in saturated fatty acids and poor in MUFA.

(Received December 15 2003)

(Revised May 18 2004)

(Accepted June 02 2004)


c1 *Corresponding author: Dr Antonis Zampelas, fax +30 210 9549141, email