World's Poultry Science Journal

Review Article

Functional patterns of exogenous enzymes in different feed ingredients

Y.M. BAOa1, L.F. ROMEROa2 and A.J. COWIESONa1 c1

a1 Poultry Research Foundation, Veterinary Science Faculty, University of Sydney, Camden, NSW 2570, Australia

a2 Danisco Animal Nutrition – DuPont Industrial Biosciences, Marlborough, Wiltshire, SN81XN, United Kingdom


The use of carbohydrases and proteases in poultry production occasionally results in inconsistent physiological responses that are difficult to explain. However, it has previously been demonstrated that a substantial portion of the variation in enzyme efficacy is associated with the inherent ileal digestibility of starch, protein and lipid, and so an appreciation for the concentration of the undigested fractions of these nutrients in a diet may help to determine the magnitude and consistency of feed enzyme responses. Considering that the expense of in vivo physiological assays for the assessment of enzyme functional pattern is high, it is crucial to develop suitable in vitro methods to predict the magnitude of enzyme response. The purpose of the current paper is to describe inherent characteristics of poultry diets and feed ingredients in response to carbohydrases and proteases in order to aid the development of in vitro assays to predict feed enzyme functional patterns.

(Received November 27 2012)

(Accepted February 26 2013)

Key Words:

  • carbohydrases;
  • in vitro study;
  • ileal digestibility;
  • broilers


c1 Corresponding author: