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Evaluation of the protective effects of α-tocopherol and retinol against ochratoxin A cytotoxicity

Published online by Cambridge University Press:  09 March 2007

A. Baldi ,
M. N. Losio ,
F. Cheli ,
R. Rebucci ,
L. Sangalli ,
E. Fusi ,
B. Bertasi ,
E. Pavoni ,
S. Carli  and
I. Politis
A. Baldi*
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
M. N. Losio
Affiliation:
Istituto Zooprofilattico Sperimentale (IZS) della Lombardia e dell'Emilia Romagna, 25100, Brescia, Italy
F. Cheli
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
R. Rebucci
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
L. Sangalli
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
E. Fusi
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
B. Bertasi
Affiliation:
Istituto Zooprofilattico Sperimentale (IZS) della Lombardia e dell'Emilia Romagna, 25100, Brescia, Italy
E. Pavoni
Affiliation:
Istituto Zooprofilattico Sperimentale (IZS) della Lombardia e dell'Emilia Romagna, 25100, Brescia, Italy
S. Carli
Affiliation:
Department of Veterinary Sciences and Technology for Food Safety, Veterinary Medicine Faculty, 20133, Milan, Italy
I. Politis
Affiliation:
Department of Animal Production, Agricultural University of Athens, Athens, Greece11855
*
*Corresponding author: Dr Antonella Baldi, fax +39 02 50315746, email antonella.baldi@unimi.it
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Abstract

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Ochratoxin A (OTA), a mycotoxin frequently present in food and feedstuffs, produces a wide range of toxic effects, including cell death via lipid peroxidation. In one human and four animal cell lines we determined the half lethal concentration (LC50) of OTA, its effect on reactive oxygen species (ROS) production, and its ability to induce cytochrome p450 activity. We also examined the protective effect of α-tocopherol and all-trans-retinol in the most sensitive cell lines (i.e. bovine mammary epithelia, for which LC50 was 0·8μg/ml (24h), and Madin Darby canine kidney, for which LC50 was 4·3μg/ml (48h)). Pre-incubation for 3h with either antioxidant significantly (P<0·05) ameliorated the OTA-induced reduction in cell viability and significantly decreased (P<0·05) ROS production. These findings indicate that oxidative stress is an important factor in OTA cytotoxicity. Supplementation with antioxidant molecules may counteract the short-term toxicity of this mycotoxin.

Keywords

Ochratoxin AAntioxidantsCell culturesReactive oxygen species
Type
Short communication
Information
British Journal of Nutrition , Volume 91 , Issue 4 , April 2004 , pp. 507 - 512
Copyright
Copyright © The Nutrition Society 2004

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