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Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible

Published online by Cambridge University Press:  01 November 1997

SHUNICHI SHIBATA ,
KENJI FUKADA ,
SHOICHI SUZUKI  and
YASUO YAMASHITA
SHUNICHI SHIBATA
Affiliation:
1st Department of Oral Anatomy, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan
KENJI FUKADA
Affiliation:
2nd Department of Orthodontics, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan
SHOICHI SUZUKI
Affiliation:
2nd Department of Orthodontics, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan
YASUO YAMASHITA
Affiliation:
1st Department of Oral Anatomy, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan
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Abstract

We investigated the immunohistochemical localisation of types II and X collagen as well as the cytochemical localisation of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible on d 14–16 of pregnancy. On d 14 of pregnancy, although no immunostaining for types II and X collagen was observed, alkaline phosphatase activity was detected in all cells in the anlage of the future condylar process. On d 15 of pregnancy, immunostaining for both collagen types was simultaneously detected in the primarily formed condylar cartilage. Alkaline phosphatase activity was also detected in chondrocytes at this stage. By d 16 of pregnancy, the hypertrophic cell zone rapidly increased in size. These findings strongly support a periosteal origin for the condylar cartilage of the fetal mouse mandible, and show that progenitor cells for condylar cartilage rapidly or directly differentiate into hypertrophic chondrocytes.

Keywords

Boneperiosteumchondrocytes
Type
Research Article
Information
The Journal of Anatomy , Volume 191 , Issue 4 , November 1997 , pp. 561 - 570
Copyright
© Anatomical Society of Great Britain and Ireland 1997

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