British Journal of Nutrition

Research Article

Eicosapentaenoic and docosapentaenoic acids are the principal products of α-linolenic acid metabolism in young men*

Graham C. Burdgea1 c1, Amanda E. Jonesa1 and Stephen A. Woottona1

a1 Institute of Human Nutrition, Level C, West Wing, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK


The capacity for conversion of α-linolenic acid (ALNA) to n−3 long-chain polyunsaturated fatty acids was investigated in young men. Emulsified [U13C]ALNA was administered orally with a mixed meal to six subjects consuming their habitual diet. Approximately 33 % of administered [13C]ALNA was recovered as 13CO2 on breath over the first 24 h. [13C]ALNA was mobilised from enterocytes primarily as chylomicron triacylglycerol (TAG), while [13C]ALNA incorporation into plasma phosphatidylcholine (PC) occurred later, probably by the liver. The time scale of conversion of [13C]ALNA to eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA) suggested that the liver was the principal site of ALNA desaturation and elongation, although there was some indication of EPA and DPA synthesis by enterocytes. [13C]EPA and [13C]DPA concentrations were greater in plasma PC than TAG, and were present in the circulation for up to 7 and 14 d, respectively. There was no apparent 13C enrichment of docosahexaenoic acid (DHA) in plasma PC, TAG or non-esterified fatty acids at any time point measured up to 21 d. This pattern of 13C n−3 fatty acid labelling suggests inhibition or restriction of DHA synthesis downstream of DPA. [13C]ALNA, [13C]EPA and [13C]DPA were incorporated into erythrocyte PC, but not phosphatidylethanolamine, suggesting uptake of intact plasma PC molecules from lipoproteins into erythrocyte membranes. Since the capacity of adult males to convert ALNA to DHA was either very low or absent, uptake of pre-formed DHA from the diet may be critical for maintaining adequate membrane DHA concentrations in these individuals.

(Received July 06 2001)

(Revised April 03 2002)

(Accepted May 17 2002)


c1 Corresponding author:Dr G. C. Burdge, fax +44 23 80794945, email


* Part of this work has been presented in abstract form (Burdge GC, Jones AE, Wright P, Ware L & Wootton SA (2001) α-Linolenic acid metabolism in adult men: evidence for synthesis of eicosapentaenoic and docosapentaenoic acids, but not docosahexaenoic acid. Proceedings of the Nutrition Society 60, 22A).