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High levels of genetic diversity in Nosema ceranae within Apis mellifera colonies

Published online by Cambridge University Press:  15 November 2013

TAMARA GÓMEZ-MORACHO ,
XULIO MASIDE ,
RAQUEL MARTÍN-HERNÁNDEZ ,
MARIANO HIGES  and
CAROLINA BARTOLOMÉ
TAMARA GÓMEZ-MORACHO*
Affiliation:
Laboratorio de Patología Apícola, Centro Apícola Regional, JCCM, 19180 Marchamalo, Spain
XULIO MASIDE
Affiliation:
Departamento de Anatomía Patolóxica e Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain Grupo de Medicina Xenómica, CIMUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain Grupo de Xenómica Comparada de Parásitos Humanos, IDIS, Santiago de Compostela, Galicia, Spain
RAQUEL MARTÍN-HERNÁNDEZ
Affiliation:
Laboratorio de Patología Apícola, Centro Apícola Regional, JCCM, 19180 Marchamalo, Spain Instituto de Recursos Humanos para la Ciencia y la Tecnología (INCRECYT), Fundación Parque Científico Tecnológico de Albacete, Spain
MARIANO HIGES
Affiliation:
Laboratorio de Patología Apícola, Centro Apícola Regional, JCCM, 19180 Marchamalo, Spain
CAROLINA BARTOLOMÉ
Affiliation:
Departamento de Anatomía Patolóxica e Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain Grupo de Medicina Xenómica, CIMUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain Grupo de Xenómica Comparada de Parásitos Humanos, IDIS, Santiago de Compostela, Galicia, Spain
*
*Corresponding author: Camino de San Martín sn. 19180, Marchamalo, Spain. E-mail: tgomez@externas.jccm.es
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Summary

Nosema ceranae is a widespread honeybee parasite, considered to be one of the pathogens involved in the colony losses phenomenon. To date, little is known about its intraspecific genetic variability. The few studies on N. ceranae variation have focused on the subunits of ribosomal DNA, which are not ideal for this purpose and have limited resolution. Here we characterized three single copy loci (Actin, Hsp70 and RPB1) in three N. ceranae isolates from Hungary and Hawaii. Our results provide evidence of unexpectedly high levels of intraspecific polymorphism, the coexistence of a wide variety of haplotypes within each bee colony, and the occurrence of genetic recombination in RPB1. Most haplotypes are not shared across isolates and derive from a few frequent haplotypes by a reduced number of singletons (mutations that appear usually just once in the sample), which suggest that they have a fairly recent origin. Overall, our data indicate that this pathogen has experienced a recent population expansion. The presence of multiple haplotypes within individual isolates could be explained by the existence of different strains of N. ceranae infecting honeybee colonies in the field which complicates, and must not be overlooked, further analysis of host–parasite interactions.

Keywords

Nosema ceranaeActinHsp70RPB1genetic diversity
Type
Research Article
Information
Parasitology , Volume 141 , Issue 4 , April 2014 , pp. 475 - 481
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Copyright
Copyright © Cambridge University Press 2013 

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