Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-19T20:44:35.729Z Has data issue: false hasContentIssue false
  • English
  • Français

Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida, and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region

Published online by Cambridge University Press:  15 October 2013

Katarzyna Karpiej ,
Manon Simard ,
Erica Pufall  and
Jerzy Rokicki
Katarzyna Karpiej*
Affiliation:
University of Gdansk, Department of Invertebrate Zoology and Parasitology, Poland
Manon Simard
Affiliation:
Nunavik Research Centre, Makivik Corporation, Canada
Erica Pufall
Affiliation:
University of Guelph, Department of Population Medicine, Canada
Jerzy Rokicki
Affiliation:
University of Gdansk, Department of Invertebrate Zoology and Parasitology, Poland
*
Correspondence should be addressed to: K. Karpiej, University of Gdansk, Department of Invertebrate Zoology and Parasitology, Poland email: kkarpiej@ocean.ug.edu.pl
Get access Rights & Permissions [Opens in a new window]

Abstract

As many Arctic fish species are intermediate hosts of anisakids, they are present in the diet of the ringed seal, Pusa hispida, and the bearded seal, Erignathus barbatus. Parasitic nematodes from the stomachs of 66 seals caught in the Nunavut region (Canada) from two communities (Arviat and Sanikiluaq) from October 2007 to January 2008 have been examined in order to identify the epidemiological risk for Inuit communities who consume traditional food. In Arviat 2428 anisakids were observed in 37 seals, while in Sanikiluaq 316 Anisakidae were isolated from 29 seals. The worms were treated with a host tissue, washed in deionized water and stored until analysis in 70% ethanol. The parasites were divided into three parts. The anterior and posterior parts were stored in 70% ethanol containing 5% glycerol and were examined using a light microscope by evaporation of the ethanol/glycerin mixture. The central parts were prepared for molecular identification by fixing in 70% ethanol. Using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method, the following members of the family Anisakidae were identified: Contracaecum osculatum A and C and Pseudoterranova bulbosa. In the studied material, more adult worms were noted than larval stages. The most numerous nematodes were P. bulbosa, and mixed infection was observed. The mean prevalence of anisakids infection was 43.2% in the Arviat and 37.9% in the Sanikiluaq communities.

Keywords

ringed sealbearded sealPseudoterranova bulbosaContracaecum osculatum A and CNorth Canada
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 94 , Issue 6 , September 2014 , pp. 1237 - 1241
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Adams, A.M. (1988) Taxonomy, systematics and ecology of helminth parasites of the ringed seal, Phoca hispida Schreber, in Alaskan waters. PhD dissertation. University of Washington, Seattle, USA.Google Scholar
Brattey, J. and Stanson, G.B. (1993) Host specificity and abundance of parasitic nematodes (Ascaridoidea) from the stomachs of five phocid species from Newfoundland and Labrador. Canadian Journal of Zoology 79, 2156–2166.CrossRefGoogle Scholar
Chambellant, M. (2010) Hudson Bay ringed seal: ecology in a warming climate. In Ferguson, S.H., Loseto, L.L. and Mallory, M.L. (eds) A little less Arctic: top predators in the world's largest northern inland sea, Hudson Bay. New York: Springer, pp. 137–158.CrossRefGoogle Scholar
Chen, Q., Yu, H.Q., Lun, Z.R., Chen, X.G., Song, H.Q., Lin, R.Q. and Zhu, X.Q. (2008) Specific PCR assays for the identification of common anisakid nematodes with zoonotic potential. Parasitology Research 104, 79–84.CrossRefGoogle ScholarPubMed
Couture, C., Measures, L., Gagnon, J. and Desbiens, C. (2003) Human intestinal anisakidosis due to consumption of raw salmon. American Journal of Surgical Pathology 27, 1167–1172.CrossRefGoogle Scholar
D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanco, M. and Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223–226.CrossRefGoogle ScholarPubMed
Fagerholm, H.P. (1991) Systematic implications of male caudal morphology in ascaridoid nematode parasites. Systematic Parasitology 19, 215–228.CrossRefGoogle Scholar
Finley, K.J. and Evans, C.R. (1983) Summer diet of the bearded seal (Erignathus barbatus) in the Canadian high Arctic. Arctic 36, 82–89.CrossRefGoogle Scholar
Hoarau, G., Holla, S., Lescasse, R., Stam, W.T. and Olsen, J.L. (2002) Heteroplasmy and evidence for recombination in the mitochondrial control region of the flatfish Platichthys flesus. Molecular Biology and Evolution 19, 2261–2264.CrossRefGoogle ScholarPubMed
Hopping, B.N., Mead, E., Erber, E., Sheehy, C., Roache, C. and Sharma, S. (2010) Dietary adequacy of Inuit in the Canadian Arctic. Journal of Human Nutrition and Dietetics 23, 27–34.CrossRefGoogle ScholarPubMed
Johansen, C.E., Lydersen, C., Aspholm, P.E., Haug, T. and Kovacs, K.M. (2010) Helminth parasites in ringed seals (Pusa hispida) from Svalbard, Norway with special emphasis on nematodes: variation with age, sex, diet, and location of host. Journal of Parasitology 96, 946–953.CrossRefGoogle ScholarPubMed
Kijewska, A., Dzido, J., Shukhgalter, O. and Rokicki, J. (2009) Anisakid parasites of fishes caught on the African shelf. Journal of Parasitology 95, 639–645.CrossRefGoogle ScholarPubMed
Kijewska, A., Rokicki, J., Sitko, J. and Węgrzyn, G. (2002) Ascaridoidea: a simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds. Experimental Parasitology 101, 35–39.CrossRefGoogle ScholarPubMed
Mattiucci, S. and Nascetti, G. (2008) Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host–parasite co-evolutionary processes. Advances in Parasitology 66, 48–148.Google ScholarPubMed
Mattiucci, S., Paggi, L., Nascetti, G., Ishikura, H., Kikuchi, K., Sato, N., Cianchi, R. and Bullini, L. (1998) Allozyme and morphological identification of Anisakis, Contracaecum and Pseudoterranova from Japanese waters (Nematoda, Ascaridoidea). Systematic Parasitology 40, 81–92.CrossRefGoogle Scholar
McClelland, G. (1980) Phocanema decipiens: pathology in seals. Experimental Parasitology 49, 405–419.CrossRefGoogle ScholarPubMed
Measures, L.N. and Gosselin, J.-F. (1994) Helminth parasites of ringed seal, Phoca hispida, from Northern Quebec, Canada. Journal of the Helminthological Society of Washington 61, 240–244.Google Scholar
Myers, B.J. (1957) Ascaroid parasites of harp seals (Phoca gröenlandica Erxleben) from the Magdalen Islands, Quebec. Canadian Journal of Zoology 35, 291–292.Google Scholar
Nascetti, G., Cianchi, R., Mattiucci, S., D'Amelio, S., Orecchia, P., Paggi, L., Brattey, J., Berland, B., Smith, J.W. and Bullini, L. (1993) Three sibling species within Contracaecum osculatum (Nematoda, Ascaridida, Ascaridoidea) from the Atlantic Arctic–Boreal region: reproductive isolation and host preferences. International Journal for Parasitology 23, 105–120.CrossRefGoogle ScholarPubMed
Rózsa, L., Reiczigel, J. and Majoros, G. (2000) Quantifying parasites in samples of hosts. Journal of Parasitology 86, 228–232.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Valtonen, E.T., Fagerholm, H.-P. and Helle, E. (1988) Contracaecum osculatum (Nematoda: Anisakidae) in fish and seals in Bothnian Bay (northeastern Baltic Sea). International Journal for Parasitology 18, 365–370.CrossRefGoogle ScholarPubMed
Zhu, X.Q., Gasser, R.B., Podolska, M. and Chilton, N.B. (1998) Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 1911–1921.CrossRefGoogle ScholarPubMed