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Life cycle of Cryptosporidium muris in two rodents with different responses to parasitization

Published online by Cambridge University Press:  11 October 2013

JANKA MELICHEROVÁ ,
JANA ILGOVÁ ,
MARTIN KVÁČ ,
BOHUMIL SAK ,
BŘETISLAV KOUDELA  and
ANDREA VALIGUROVÁ
JANKA MELICHEROVÁ
Affiliation:
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
JANA ILGOVÁ
Affiliation:
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
MARTIN KVÁČ
Affiliation:
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic
BOHUMIL SAK
Affiliation:
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic
BŘETISLAV KOUDELA
Affiliation:
Department of Pathological Morphology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42 Brno, Czech Republic
ANDREA VALIGUROVÁ*
Affiliation:
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
*
*Corresponding author: Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic. E-mail: andreav@sci.muni.cz
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Summary

This study focuses on mapping the life cycle of Cryptosporidium muris in two laboratory rodents; BALB/c mice and the southern multimammate rat Mastomys coucha, differing in their prepatent and patent periods. Both rodents were simultaneously experimentally inoculated with viable oocysts of C. muris (strain TS03). Animals were dissected and screened for the presence of the parasite using a combined morphological approach and nested PCR (SSU rRNA) at different times after inoculation. The occurrence of first developmental stages of C. muris in stomach was detected at 2·5 days post-infection (dpi). The presence of Type II merogony, appearing 36 h later than Type I merogony, was confirmed in both rodents. Oocysts exhibiting different size and thickness of their wall were observed from 5 dpi onwards in stomachs of both host models. The early phase of parasitization in BALB/c mice progressed rapidly, with a prepatent period of 7·5–10 days; whereas in M. coucha, the developmental stages of C. muris were first observed 12 h later in comparison with BALB/c mice and prepatent period was longer (18–21 days). Similarly, the patent periods of BALB/c mice and M. coucha differed considerably, i.e. 10–15 days vs chronic infection throughout the life of the host, respectively.

Keywords

cryptosporidiadevelopmentgastricoocystpathologyType II merogony
Type
Research Article
Information
Parasitology , Volume 141 , Issue 2 , February 2014 , pp. 287 - 303
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Copyright
Copyright © Cambridge University Press 2013 

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