British Journal of Nutrition

Full Papers

Nutritional Immunology

Dietary supplementation with Bifidobacterium lactis NCC2818 from weaning reduces local immunoglobulin production in lymphoid-associated tissues but increases systemic antibodies in healthy neonates

Marie C. Lewisa1 c1, Dilip V. Patela1, Jenni Fowlera1, Swantje Dunckera2, Adrian W. Zuerchera2, Annick Merceniera2 and Mick Baileya1

a1 School of Veterinary Science, Bristol University, Langford House, Langford BS40 5DU, UK

a2 Allergy Group, Nutrition and Health Department, Nestlé Research Center, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland

Abstract

Weaning is associated with a major shift in the microbial community of the intestine, and this instability may make it more acquiescent than the adult microbiota to long-term changes. Modulation achieved through dietary interventions may have potentially beneficial effects on the developing immune system, which is driven primarily by the microbiota. The specific aim of the present study was to determine whether immune development could be modified by dietary supplementation with the human probiotic Bifidobacterium lactis NCC2818 in a tractable model of weaning in infants. Piglets were reared by their mothers before being weaned onto a solid diet supplemented with B. lactis NCC2818, while sibling controls did not receive supplementation. Probiotic supplementation resulted in a reduction in IgA (P< 0·0005) and IgM (P< 0·009) production by mucosal tissues but had no effect on IgG production (P>0·05). Probiotic-supplemented pigs had more mast cells than unsupplemented littermates (P< 0·0001), although numbers in both groups were low. In addition, the supplemented piglets made stronger serum IgG responses to fed and injected antigens (P< 0·05). The present findings are consistent with B. lactis NCC2818 reducing intestinal permeability induced by weaning, and suggest that the piglet is a valuable intermediate between rodent models and human infants. The results also strongly suggest that measures of the effect of probiotic supplementation on the immune system need to be interpreted carefully as proxy measures of health benefit. However, they are useful in developing an understanding of the mechanism of action of probiotic strains, an important factor in predicting favourable health outcomes of nutritional intervention.

(Received July 12 2012)

(Revised November 09 2012)

(Accepted January 07 2013)

(Online publication March 11 2013)

Key Words:

  • Dietary supplementation;
  • Immune development;
  • Mucosal immunology;
  • Weaning

Correspondence

c1 Corresponding author: Dr M. C. Lewis, fax +44 1179 289 505, email marie.lewis@bristol.ac.uk

Footnotes

  Abbreviations: MLN, mesenteric lymph node; PP, Peyer's patches

0Comments