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Evaluation of fermentability of acid-treated maize husk by rat caecal bacteria in vivo and in vitro

Published online by Cambridge University Press:  09 March 2007

Hiroshi Hara ,
Yutaka Saito ,
Hiroshi Nakashima  and
Shuh Achi Kiriyama
Hiroshi Hara
Affiliation:
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Yutaka Saito
Affiliation:
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Hiroshi Nakashima
Affiliation:
Development Department Pharmaceutical Section, Fujinomiya Factory, Terumo Corporation. Fujinomiya-city, Shizuoka 418, Japan
Shuh Achi Kiriyama
Affiliation:
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
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Abstract

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Fermentable energy in insoluble dietary fibre (DF) sources was evaluated by in vivo and in vitro methods using rats. Test diets contained 50 and 100 g maize husk or organic-acid-treated maize husk/kg diet. Soluble fractions were removed from both the DF sources by washing. The acid treatment increased digestibility by a microbial hemicellulase from 12.7% to 32.6%. The fermentabiliry of DF was evaluated by measurement of the production rate of short-chain fatty acids (SCFA) in a short-term in vitro incubation of the caecal contents of rats fed on test diets for 22 d. The production rates of the major SCFA, acetic, propionic and butyric acids, were increased by feeding both DF sources, and these production rates in the acid-treated DF group were significantly higher than those in the untreated DF group. The production rate of a minor SCFA, isovaleric acid, was decreased by feeding both diets. The production rate of total SCFA in rats given the acid-treated maize husk was 32.6% higher than that in rats given the untreated maize husk. The fermentable energy in DF was estimated in vivo by subtracting the faecal excretion of DF energy from ingested DF energy. The fermentable energy in DF was increased by the acid treatment (32·5% in maize husk and 63.4% in acid-treated maize husk), which agreed with the SCFA production rate predicted in the caecum. These results indicate that a short-term incubation of caecal contents is a useful method for evaluation of the fermentability of DF sources, and that acid treatment can increase the fermentability of an insoluble DF source.

Keywords

Short-chain fatty acidsDietary fibreCaecumRat
Type
Fermentality of maize husks in the caeca of rats
Information
British Journal of Nutrition , Volume 71 , Issue 5 , May 1994 , pp. 719 - 729
Copyright
Copyright © The Nutrition Society 1994

References

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.Google Scholar
American Institute of Nutrition (1980). Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.CrossRefGoogle Scholar
Argenzio, R. A., Miller, N. & von Engelhardt, W. (1975). Effect of volatile fatty acids on water and ion absorption from the goat colon. American Journal of Physiology 229, 997–1002.Google Scholar
Association of Official Analytical Chemists (1990). Oficial Methods of Analysis, 15th ed. Arlington, Virginia: AOAC.Google Scholar
Carroll, E. J. & Hungate, R. E. (1954). The magnitude of the microbial fermentation in the bovine rumen. Applied Microbiology 2, 205–214.Google Scholar
Costa, M. A., Mehta, T. & Males, J. R. (1989). Effects of dietary cellulose and psyllium husk on monkey colonic microbial metabolism in continuous culture. Journal of Nutrition 119, 979–985.Google Scholar
Cummings, J. H., Pomare, E. W., Branch, W. J., Naylor, C. P. E. & Macfarlane, G. T. (1987). Short chain fatty acids in human large intestine, portal, hepatic and venous blood. >Gut 28, 1221–1227.Google ScholarPubMed
Fleming, S. E., Fitch, M. D. & DeVries, S. (1992). The influence of dietary fibre on proliferation of intestinal mucosal cells in miniature swine may not be mediated primarily by fermentation. Journal of Nutrition 122, 906–916.CrossRefGoogle Scholar
Folch, J., Lees, M. & Sloan-Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Harper, A. E. (1959). Amino acid balance and imbalance. 1. Dietary level of protein and amino acid imbalance. Journal of Nutrition 68, 405–418.CrossRefGoogle Scholar
Hoover, W. H. & Heitmann, R. N. (1972). Effects of dietary fiber levels on weight gain, cecal volume and volatile fatty acid production in rabbits. Journal of Nutrition 102, 375–380.CrossRefGoogle ScholarPubMed
Johnson, J. L. & McBee, R. H. (1967). The porcupine cecal fermentation. Journal 0f Nutririon 91, 540–546.Google ScholarPubMed
Koehler, L. H. (1952). Differentiation of carbohydrates by anthrone reaction rate and color intensity. Analytical Chemistry 24, 1576–1579.CrossRefGoogle Scholar
Lowry, O.H., Rosebrough, H. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Fohn-phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Mathers, J. C. & Dawson, L. D. (1991). Large bowel fermentation in rats eating processed potatoes. British Journal of Nutrition 66, 313–329.Google Scholar
McBurney, M. I. & Thompson, L. U. (1987). Effect of human faecal inoculum on in vitro fermentation variables. British Journal of Nutrition 58, 233–243.Google Scholar
Parker, D. S. (1976). The measurement of the production rates of volatile fatty acids in the caecum of the conscious rabbit. British Journal of Nutrition 36, 61–70.Google Scholar
Prosky, L., Asp, N.-G., Furda, I., Devries, J. W., Schweizer, T. F. & Harland, B. F. (1985). Determination of total dietary fibre in foods and food products, collaborative study. Journal of the Association of Official Analytical Chemists 68, 677–679.Google Scholar
Reeves, P. G. (1989). AIN-76 diet, should we change the formulation? Journal of Nutrition 119, 1081–1082.CrossRefGoogle ScholarPubMed
Rémésy, C. & Demigne, C. (1976). Partition and absorption of volatile fatty acids in the alimentary canal of the rats. Annals de Recherches Vétérinaires 7, 39–55.Google Scholar
Robertson, J. A., Murison, S. D. & Chesson, A. (1987). Estimation of the potential digestibility and rate of degradation of water-insoluble dietary fiber in the pig cecum with a modified nylon bag technique. Journal of Nutrition 117, 1402–1409.CrossRefGoogle ScholarPubMed
Sakata, T. (1987). Stimulatory effect of short-chain fatty acids in epithelial cell proliferation in the rat intestine: a possible explanation for trophic effects of fermentable fibre, gut microbes and luminal trophic factors. British Journal of Nutrition 58, 95–103.Google Scholar
Stanogias, G. & Pearce, G. R. (1985). The digestion of fibre by pigs. 2. Volatile fatty acid concentrations in large intestine digesta. British Journal of Nutrition 53, 531–536.Google Scholar
Sugawara, K. (1975). Influence of triton X- 100 on protein determination by Lowry procedure. Agricultural and Biological Chemistry 93, 2429–2430.Google Scholar
Tinker, L. F. & Schneeman, B. O. (1989). The effects of guar gum or wheat bran on the disappearance of “C- labeled starch from the rat gastrointestinal tract. Journal of Nutrition 119, 403–408.CrossRefGoogle ScholarPubMed
Van Nevel, C. J. & Demeyer, D. I. (1988). Manipulation of rumen fermentation. In The Rumen Microbial Ecosystem, pp. 387443 [Hobson, P. N., editor]. London and New York: Elsevier Applied Science.Google Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. 11. A rapid method for the determination of fibre and lignin. Journal of the Association of Official Agricultural Chemists 46, 829–835.Google Scholar
Van Soest, P. J. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Analytical Chemists 50, 50–55.Google Scholar
Wolever, T. M. S., Spadafora, P. & Eshunis, H. (1991). Interaction between colonic acetate and propionate in humans. American Journal of Clinical Nutrition 63, 681–687.CrossRefGoogle Scholar
Yajima, T. (1985). Contractile effect of short-chain fatty acids on the isolated colon of the rat. Journal of Physiology (London) 368, 667–678.CrossRefGoogle ScholarPubMed
Yang, M. G., Manoharan, K. & Mickelsen, O. (1970). Nutritional contribution of volatile fatty acids from the cecum of rats. Journal of Nutrition 100, 545–550.CrossRefGoogle ScholarPubMed
Zarling, E. J. & Ruchim, M. A. (1987). Protein origin of the volatile fatty acids isobutyrate and isovalerate in human stool. Journal of Laboratory and Clinical Medicine 109, 566–570.Google Scholar