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Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si

Published online by Cambridge University Press:  15 July 2013

Giorgi Shtenberg ,
Naama Massad-Ivanir ,
Oren Moscovitz ,
Sinem Engin ,
Michal Sharon ,
Ljiljana Fruk  and
Ester Segal
Giorgi Shtenberg
Affiliation:
The Inter-Departmental Program of Biotechnology, Technion – Israel Institute of Technology, Haifa 32000, Israel
Naama Massad-Ivanir
Affiliation:
Department of Biotechnology and Food Engineering, Technion – Israel Institute of Technology, Haifa 32000, Israel
Oren Moscovitz
Affiliation:
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Sinem Engin
Affiliation:
Karlsruhe Institute of Technology, DFG – Center for Functional Nanostructures, Karlsruhe 76131, Germany
Michal Sharon
Affiliation:
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Ljiljana Fruk
Affiliation:
Karlsruhe Institute of Technology, DFG – Center for Functional Nanostructures, Karlsruhe 76131, Germany
Ester Segal
Affiliation:
Department of Biotechnology and Food Engineering, Technion – Israel Institute of Technology, Haifa 32000, Israel The Russell Berrie Nanotechnology Institute, Technion – Israel Institute of Technology, Haifa 32000, Israel
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Abstract

Optical biosensor for monitoring proteolytic activity is constructed by DNA-directed immobilization of enzymes onto porous Silicon nanostructures. This sensor configuration allows both protease recycling and easy surface regeneration for subsequent biosensing analysis by means of mild dehybridization conditions. We demonstrate real-time analysis of minute quantities of proteases paving the way for substrate profiling and the identification of cleavage sites. The biosensor is compatible with common proteomic methods and allows for a successful downstream mass spectrometry analysis of the reaction products.

Keywords

optical propertiessensormass spectroscopy
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium NN – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 195 - 200
Copyright
Copyright © Materials Research Society 2013 

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References

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