Epidemiology and Infection

Original Papers

Other paper

The emergence of wildlife species as a source of human rabies infection in Brazil

S. R. FAVORETTOa1a2 c1, C. C. de MATTOSa3, C. A. de MATTOSa3, A. C. A. CAMPOSa2, D. R. V. SACRAMENTOa2a4 and E. L. DURIGONa2

a1 Instituto Pasteur de São Paulo, São Paulo, Brazil

a2 Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil

a3 Centers for Disease Control and Prevention, Atlanta, GA, USA

a4 Genomic Molecular Engineering Laboratory, São Paulo, Brazil

SUMMARY

Forty-five human rabies virus isolates from a wide geographical area of Brazil were characterized using an anti-nucleoprotein monoclonal antibody panel and by partial nucleotide sequencing analysis of the nucleoprotein gene. Three major antigenic groups related to the antigenic variants maintained in domestic dogs, vampire bats and marmosets were identified. Phylogenetic analyses revealed that the viruses from dog-related cases segregated into four sister clades: three associated with dog-endemic cycles in Brazil and one with the crab-eating fox cycle in the northeastern region of the country. The vampire bat- and marmoset-related viruses formed two independent groups. The topology of these clades was conserved when these samples were compared to virus representatives of the currently reported rabies endemic cycles in the Americas. These results indicated the presence of multiple endemic transmission cycles maintained in four different reservoirs, domestic dogs, crab-eating foxes, vampire bats and marmosets, which are being transmitted directly to humans and should be considered as a high-risk for rabies infection.

(Received June 22 2012)

(Revised December 11 2012)

(Accepted January 14 2013)

(Online publication February 22 2013)

Key words

  • Host (in infections);
  • molecular biology;
  • rabies (human);
  • virus infection;
  • zoonoses

Correspondence

c1 Address for correspondence: S. R. Favoretto, Ph.D., Instituto Pasteur of São Paulo, Brazil, Av. Paulista, 393 Paraíso, São Paulo, SP, Brasil, CEP 01311000. (Email: srfavoretto@usp.br)

Metrics