Physiology and functional biology of systems

Effect of butaphosphan and cyanocobalamin on postpartum metabolism and milk production in dairy cows

R. A. Pereiraa1 c1, P. A. S. Silveiraa1, P. Montagnera1, A. Schneidera1, E. Schmitta1a2, V. R. Rabassaa1, L. F. M. Pfeifera1a2, F. A. B. Del Pinoa1a3, M. E. Pulgaa4 and M. N. Corrêaa1

a1 Núcleo de Pesquisa, Ensino e Extensão em Pecuária (NUPEEC), Departamento de Clínicas Veterinária, Universidade Federal de Pelotas, CEP: 96010-900, Pelotas, Rio Grande do Sul, Brazil

a2 Centro de Pesquisa Agroflorestal de Rondônia – Embrapa CPAF, BR 364 - Km 5,5 - Zona Rural, Caixa Postal 127 - CEP 76815-800, Porto Velho, Rondônia, Brazil

a3 Departamento de Bioquímica, Universidade Federal de Pelotas, CEP: 96010-900, Pelotas, Rio Grande do Sul, Brazil

a4 Bayer S. A. Animal Health, Rua Domingos Jorge, 1100 - Prédio 9701, CEP: 04779-900, São Paulo, SP, Brazil


The aim of this study was to determine the effect of butaphosphan and cyanocobalamin (BTPC) supplementation on plasma metabolites and milk production in postpartum dairy cows. A total of fifty-two Holstein cows were randomly assigned to receive either: (1) 10 ml of saline (NaCl 0.9%, control group); (2) 1000 mg of butaphosphan and 0.5 mg of cyanocobalamin (BTPC1 group); and (3) 2000 mg of butaphosphan and 1.0 mg of cyanocobalamin (BTPC2 group). All cows received injections every 5 days from calving to 20 days in milk (DIM). Blood samples were collected every 15 days from calving until 75 DIM to determine serum concentration of glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), cholesterol, urea, calcium (Ca), phosphorus (P), magnesium (Mg), aminotransferase aspartate (AST) and γ-glutamyltransferase (GGT). The body condition score (BCS) and milk production were evaluated from calving until 90 DIM. Increasing doses of BTPC caused a linear reduction in plasma concentrations of NEFA and cholesterol. Supplementation of BTPC also reduced concentrations of BHB but it did not differ between the two treatment doses. Milk yield and milk protein had a linear increase with increasing doses of BTPC. A quadratic effect was detected for milk fat and total milk solids according to treatment dose, and BTPC1 had the lowest mean values. Concentrations of glucose, urea, P, Mg, AST, GGT, milk lactose and BCS were not affected by treatment. These results indicate that injections of BTPC during the early postpartum period can reduce NEFA and BHB concentrations and increase milk production in Holstein cows.

(Received June 17 2011)

(Accepted November 26 2012)

(Online publication January 29 2013)


  • butaphosphan;
  • cyanocobalamin;
  • postpartum dairy cows;
  • non-esterified fatty acid


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