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Molecular characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran

Published online by Cambridge University Press:  13 June 2012

S. Khademvatan ,
E. Yousefi ,
A. Rafiei ,
M. Rahdar  and
J. Saki
S. Khademvatan
Affiliation:
Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Cellular and Molecular Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
E. Yousefi
Affiliation:
Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
A. Rafiei
Affiliation:
Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
M. Rahdar
Affiliation:
Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
J. Saki*
Affiliation:
Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
*
*Fax: (+98 611) 3332036 E-mail: Khademvatan@yahoo.com
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Abstract

Hydatidosis is an important public health problem in several parts of Iran. The aim of this molecular study is to investigate Echinococcus granulosus genotypes as the causative agents of hydatidosis in the south-west of Iran (Khuzestan province). In this study, isolates of 334 hydatid cysts were collected from the liver and lungs of 141 sheep, 104 cattle, 84 goats and 5 human cases. DNA was extracted and examined by nested polymerase chain reaction (PCR) of ribosomal DNA (rDNA) internal transcribed spacer 1 (ITS1) and restriction fragment length polymorphism (RFLP)-PCR. In addition, fragments of genes coding for ITS1 were sequenced. The results of RFLP-PCR analysis revealed the presence of the G1 genotype in all human, cattle, goat and sheep isolates. Furthermore, no camel strain (G6) was detected among all samples in the regions studied. The molecular findings indicate that the predominant genotype involved in E. granulosus transmission in south-west Iran is the common sheep strain (G1 genotype), which occurs in human, cattle, sheep and goat populations. In conclusion, these results may have important implications for hydatid disease control in the areas studied.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 2 , June 2013 , pp. 240 - 244
Copyright
Copyright © Cambridge University Press 2012 

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References

Ahmadi, N. (2005) Hydatidosis in camels (Camelus dromedarius) and their potential role in the epidemiology of Echinococcus granulosus in Iran. Journal of Helminthology 79, 119–125.CrossRefGoogle ScholarPubMed
Ahmadi, N. & Dalimi, A. (2006) Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infection, Genetics and Evolution 6, 85–90.CrossRefGoogle ScholarPubMed
Bagheri, R., Haghi, S.Z., Amini, M., Fattahi, A.S. & Noorshafiee, S. (2011) Pulmonary hydatid cyst: analysis of 1024 cases. General Thoracic and Cardiovascular Surgery 59, 105–109.CrossRefGoogle ScholarPubMed
Bowles, J., Blair, D. & McManus, D.P. (1995) A molecular phylogeny of the genus Echinococcus. Parasitology 110, 317–328.CrossRefGoogle ScholarPubMed
Dakkak, A. (2010) Echinococcosis/hydatidosis: a severe threat in Mediterranean countries. Veterinary Parasitology 174, 2–11.CrossRefGoogle ScholarPubMed
Dalimi, A., Motamedi, G., Hosseini, M., Mohammadian, B., Malaki, H., Ghamari, Z. & Ghaffarifar, F. (2002) Echinococcosis/hydatidosis in western Iran. Veterinary Parasitology 105, 161–171.CrossRefGoogle ScholarPubMed
Eckert, J. & Deplazes, P. (2004) Biological, epidemiological and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clinical Microbiology Reviews 17, 107–135.CrossRefGoogle ScholarPubMed
Hadighi, R., Mirhadi, F. & Rokni, M.B. (2003) Evaluation of a dot-ELISA for the serodiagnosis of human hydatid disease. Pakistan Journal of Medical Sciences 19, 268–271.Google Scholar
Harandi, M.F., Hobbs, R.P., Adams, P.J., Mobedi, I., Morgan-Ryan, U.M. & Thompson, R.C. (2002) Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 125, 367–373.Google ScholarPubMed
Himsworth, C.G., Jenkins, E., Hill, J.E., Nsungu, M., Ndao, M., Andrew Thompson, R.C., Covacin, C., Ash, A., Wagner, B.A., McConnell, A., Leighton, F.A. & Skinner, S. (2010) Emergence of sylvatic Echinococcus granulosus as a parasitic zoonosis of public health concern in an indigenous community in Canada. American Journal of Tropical Medicine and Hygiene 82, 643–645.CrossRefGoogle Scholar
Hüttner, M., Wassermann, T., Siefert, L., Boomker, J.D.F., Dinkel, A., Sako, Y., Mackenstedt, U., Romig, T. & Ito, A. (2008) Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda: Taeniidae) from the African lion. International Journal for Parasitology 38, 861–868.CrossRefGoogle Scholar
Karcher, S.J. (1995) Polymerase chain reaction in molecular biology, a project approach. 1st edn. pp. 79–110. San Diego, Academic Press.Google Scholar
Kedra, A.H., Swiderski, Z., Tkach, V.V., Dubinsky, P., Pawlowski, Z., Stefaniak, J. & Pawlowski, J. (1999) Genetic analysis of Echinococcus granulosus from humans and pigs in Poland, Slovakia and Ukraine. A multicenter study. Acta Parasitologica 44, 248–254.Google Scholar
Kia, E.B., Rahimi, H., Sharbatkhori, M., Talebi, A., Fasihi Harandi, M. & Mirhendi, H. (2010) Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitology Research 107, 757–760.CrossRefGoogle ScholarPubMed
Lymbery, A.J. & Thompson, R.C. (1988) Electrophoretic analysis of genetic variation in Echinococcus granulosus from domestic hosts in Australia. International Journal for Parasitology 18, 803–811.CrossRefGoogle ScholarPubMed
McManus, D.P. & Bowles, J. (1996) Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687–704.CrossRefGoogle ScholarPubMed
Moks, E., Jogisalu, I., Valdmann, H. & Saarma, U. (2008) First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of ‘genotypes’ G5–G10. Parasitology 135, 647–654.CrossRefGoogle Scholar
Nakao, M., McManus, D.P., Schantz, P.M., Craig, P.S. & Ito, A. (2007) Echinococcus inferred from complete mitochondrial genomes. Parasitology 134, 713–722.CrossRefGoogle ScholarPubMed
Parsa, F., Haghpanah, B., Pestechian, N. & Salehi, M. (2011) Molecular epidemiology of Echinococcus granulosus strains in domestic herbivores of Lorestan, Iran. Jundishapur Journal of Microbiology 4, 123–130.Google Scholar
Pour, A.A., Hosseini, S.H. & Shayan, P. (2011) Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitology Research 108, 1229–1234.CrossRefGoogle ScholarPubMed
Rafiei, A., Hemadi, A., Maraghi, S., Kaikhaei, B. & Craig, P.S. (2007) Human cystic echinococcosis in nomads of south-west Islamic Republic of Iran. Eastern Mediterranean Health Journal 13, 41–48.Google ScholarPubMed
Rahimi, H.R., Kia, E.B., Mirhendi, S.H., Talebi, A., Fasihi Harandi, M., Jalali-zand, N. & Rokni, M.B. (2007) A new primer pair in ITS1 region for molecular studies on Echinococcus granulosus. Iranian Journal of Public Health 36, 45–49.Google Scholar
Rokni, M. (2009) Echinococcosis/hydatidosis in Iran. Iranian Journal of Parasitology 4, 1–16.Google Scholar
Romig, T., Dinkel, A. & Mackenstedt, U. (2006) The present situation of echinococcosis in Europe. Parasitology International 55, 187–191.CrossRefGoogle ScholarPubMed
Rostami Nejad, M., Nazemalhosseini Mojarad, E., Nochi, Z., Fasihi Harandi, M., Cheraghipour, K., Mowlavi, G.R. & Zali, M.R. (2008) Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene. Journal of Helminthology 82, 343–347.CrossRefGoogle ScholarPubMed
Samadian, M., Alavi, E., Sharifi, G., Rezaee, O. & Faramarzi, F. (2010) Extension of echinococcal spinal infestation extra- and intradurally after a decade of extinction. Journal of Neurosurgical Sciences 54, 143–148.Google ScholarPubMed
Shahnazi, M., Hejazi, H., Salehi, M. & Andalib, A.R. (2011) Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Tropica 117, 47–50.CrossRefGoogle ScholarPubMed
Sharbatkhori, M., Mirhendi, H., Jex, A.R., Pangasa, A., Campbell, B.E., Kia, E.B., Eshraghian, M.R., Harandi, M.F. & Gasser, R.B. (2009) Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning phylogenetic approach. Electrophoresis 30, 2648–2655.CrossRefGoogle ScholarPubMed
Sharbatkhori, M., Mirhendi, H., Harandi, M.F., Rezaeian, M., Mohebali, M., Eshraghian, M., Rahimi, H. & Kia, E.B. (2010) Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Experimental Parasitology 124, 373–379.CrossRefGoogle ScholarPubMed
Sharbatkhori, M., Fasihi Harandi, M., Mirhendi, H., Hajialilo, E. & Kia, E.B. (2011) Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitology Research 108, 521–527.CrossRefGoogle Scholar
Sharifiyazdi, H., Oryan, A., Ahmadnia, S. & Valinezhad, A. (2011) Genotypic characterization of Iranian camel (Camelus dromedarius) isolates of Echinoccocus granulosus. Journal of Parasitology 97, 251–255.CrossRefGoogle ScholarPubMed
Thompson, R.C. (2008) The taxonomy, phylogeny and transmission of Echinococcus. Experimental Parasitology 119, 439–446.CrossRefGoogle ScholarPubMed
Utuk, A.E., Simsek, S., Koroglu, E. & McManus, D.P. (2008) Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Tropica 107, 192–194.CrossRefGoogle Scholar
Van Herwerden, L., Gasser, R.B. & Blair, D. (2000) ITS-1 ribosomal DNA sequence variants are maintained in different species and strains of Echinococcus. International Journal for Parasitology 30, 157–169.CrossRefGoogle ScholarPubMed
Zhang, L., Eslami, A., Hosseini, S.H. & McManus, D.P. (1998) Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. American Journal of Tropical Medicine and Hygiene 59, 171–174.CrossRefGoogle ScholarPubMed
Zhang, L., Hu, M., Jones, A., Allsopp, B.A., Beveridge, I., Schindler, A.R. & Gasser, R.B. (2007) Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochondrial DNA, and their relationships with other selected taeniids. Molecular and Cellular Probes 21, 379–385.CrossRefGoogle ScholarPubMed