Journal of Helminthology

Research Papers

Molecular characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran

S. Khademvatana1a2, E. Yousefia1, A. Rafieia1, M. Rahdara1 and J. Sakia1 c1

a1 Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

a2 Cellular and Molecular Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Hydatidosis is an important public health problem in several parts of Iran. The aim of this molecular study is to investigate Echinococcus granulosus genotypes as the causative agents of hydatidosis in the south-west of Iran (Khuzestan province). In this study, isolates of 334 hydatid cysts were collected from the liver and lungs of 141 sheep, 104 cattle, 84 goats and 5 human cases. DNA was extracted and examined by nested polymerase chain reaction (PCR) of ribosomal DNA (rDNA) internal transcribed spacer 1 (ITS1) and restriction fragment length polymorphism (RFLP)-PCR. In addition, fragments of genes coding for ITS1 were sequenced. The results of RFLP-PCR analysis revealed the presence of the G1 genotype in all human, cattle, goat and sheep isolates. Furthermore, no camel strain (G6) was detected among all samples in the regions studied. The molecular findings indicate that the predominant genotype involved in E. granulosus transmission in south-west Iran is the common sheep strain (G1 genotype), which occurs in human, cattle, sheep and goat populations. In conclusion, these results may have important implications for hydatid disease control in the areas studied.

(Received October 07 2011)

(Accepted May 09 2012)

(Online publication June 13 2012)

Correspondence

c1 Fax: (+98 611) 3332036 E-mail: Khademvatan@yahoo.com