Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-19T08:24:54.163Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil

Published online by Cambridge University Press:  22 February 2013

Thais G. Corrêa Sholl ,
Jailson Fulgencio de Moura ,
Paulo Henrique Ott ,
Cibele R. Bonvicino ,
Estéfane C. Reis ,
Davi Castro Tavares  and
Salvatore Siciliano
Thais G. Corrêa Sholl
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil
Jailson Fulgencio de Moura*
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil Programa de Pós Graduação em Saúde Pública e Meio Ambiente, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz—ENSP/FIOCRUZ, Brazil
Paulo Henrique Ott
Affiliation:
Universidade Estadual do Rio Grande do Sul—UERGS–Unidade Litoral Norte, Osório, Laboratório de Biologia da Conservação de Aves e Mamíferos Aquáticos (LABCAM); Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Avenida Tramandaí, Imbé, R5, 95625-000, 90440-150, Brazil
Cibele R. Bonvicino
Affiliation:
Divisão de Genética, Instituto Nacional de Câncer, Rua André Cavalcante 37, 4o andar, Centro, RJ, 20231-050, Brazil and Laboratório de Biologia e Parasitologia de Mamíferos Reservatórios, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil
Estéfane C. Reis
Affiliation:
Laboratório de Genética Marinha (LGMar), Departamento de Genética, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524-PHLC-Sala 205, Maracanã, Rio de Janeiro, RJ, 20550-013, Brazil
Davi Castro Tavares
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil PPG Ecologia e Recursos Naturais, Universidade Estadual do Norte Fluminense Darcy Ribeiro–UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ 28013-602Brazil
Salvatore Siciliano
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil
*
Correspondence should be addressed to: J. F. Moura, Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil email: jailsonfm@gmail.com
Get access

Abstract

Carcasses of whales provide much valuable information on their natural history. However, some specimens cannot be identified in the field due to the advanced state of decomposition. In this study, the DNA was extracted and the mitochondrial cytochrome b gene was amplified by polymerase chain reaction and sequenced for four carcasses of possible mysticeti (GEMM: 075, 088, 135 and GEMARS: 1302). A blast search using the nucleotide–nucleotide basic local alignment (blastn) search tool was conducted using the generated sequences. Samples GEMM 075 and GEMARS 1302 showed 98% identity to one sequence of Balaenoptera acutorostrata. Samples GEMM 088 and GEMM 135 showed 99% identity to sequences from Balaenoptera edeni and Megaptera novaeangliae, respectively. A neighbour-joining tree was generated using sequences from GenBank from all species of balaenopterid that occur on the coast of Brazil. The results showed that all carcasses analysed were correspondent to species from the family Balaenopteridae already recorded in Brazil.

Keywords

whalesBrazilcarcassstrandingscytochrome b
Type
Research Article
Information
Marine Biodiversity Records , Volume 6 , 2013 , e30
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Carr, S.M., Marshall, H.D., Johnstone, K.A., Pynn, L.M. and Stenson, G.B. (2002) How to tell a sea monster. molecular discrimination of large marine animals of the North Atlantic. Biological Bulletin. Marine Biological Laboratory, Woods Hole 202, 1–5.CrossRefGoogle Scholar
Cassens, I., Vicario, S., Waddell, V.G., Balchowsky, H., Van Belle, D., Ding, W., Fan, C., Lal Mohan, R.S., Simões-Lopes, P.C., Bastida, R., Meyer, A., Stanhope, M.J. and Milinkovitch, M.C. (2000) Independent adaptation to riverine habitats allowed survival of ancient cetacean lineages. Proceedings of the National Academy of Sciences of the United States of America 97, 11343–11347.CrossRefGoogle ScholarPubMed
Geraci, R. and Lounsbury, V.J. (2005) Marine mammals ashore: a field guide for strandings. 2nd edition. Baltimore, MD: National Aquarium and Baltimore Press.Google Scholar
Hall, A.T. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Hassel, L.B., Venturotti, A., Magalhães, F.A., Cuenca, S., Siciliano, S. and Marques, F.F. (2003) Summer sightings of dwarf minke whales (Balaenoptera acutorostrata) off eastern coast of Rio de Janeiro State, Brazil. Latin American Journal of Aquatic Mammals 2, 47–50.Google Scholar
Hermann, B. and Hummel, S. (1994) Ancient DNA: recovery and analysis of genetic material from paleontological, archaeological, museum, medical and forensic specimens. 2nd edition. New York: Springer-Verlag.CrossRefGoogle Scholar
IUCN (2012) IUCN Red list of Threatened Species. version 2012.2. Available at: www.iucnredlist.org (accessed 22 January 2013).Google Scholar
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X. and Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences of the United States of America 86, 6196–6200.Google Scholar
Kumar, S., Tamura, K. and Nei, M. (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163.CrossRefGoogle ScholarPubMed
Moura, J.F. and Siciliano, S. (2012) Stranding pattern of Bryde's whales along the south-eastern coast of Brazil. Marine Biodiversity Records 5, e73. doi: 10.1017/S1755267212000528.CrossRefGoogle Scholar
Reynolds, J. and Odell, D. (1991) Marine mammal strandings in the United States. Proceedings of the Second Marine Mammal Stranding Workshop. NOAA Technical Reports, NMFS No. 98, 166 pp.Google Scholar
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.Google Scholar
Sholl, T.G.C., Nascimento, F.F., Leoncini, O., Bonvicino, C.R. and Siciliano, S. (2008) Taxonomic identification of dolphin love charms commercialized in the Amazonian region through the analysis of cytochrome b DNA. Journal of the Marine Biological Association of the United Kingdom 88, 1207–1210.CrossRefGoogle Scholar
Siciliano, S. (1997) Características da população de baleias jubarte (Megaptera novaeangliae) da costa brasileira, com especial referência aos Bancos de Abrolhos. Master's thesis. Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, Brazil.Google Scholar
Siciliano, S., Santos, M.C.O., Vicente, A.F.C., Alvarenga, F.S., Zampirolli, E., Lailson-Brito, J., Azevedo, A.F. and Pizzorno, L.A. (2004) Strandings and feeding records of Bryde's whales (Balaenoptera edeni) in south-eastern Brazil. Journal of the Marine Biological Association of the United Kingdom 84, 857–859.CrossRefGoogle Scholar
Viricel, A. and Rosel, P.E. (2012) Evaluating the utility of cox1 for cetacean species identification. Marine Mammal Science 28, 37–62.Google Scholar
Walsh, M.Y., Odell, D.K., Young, G., Asper, E.D. and Bossart, G. (1990) Mass strandings of cetaceans. In Dierauf, L.A. (ed.) CRC handbook of marine mammal medicine: health, disease and rehabilitation. Boca Raton, FL: CRC Press, pp. 673–683.Google Scholar
Weisburd, S. (1984) Whales and dolphins use magnetic ‘roads'. Science News 126, 389.Google Scholar
Zerbini, A.N., Secchi, E.R., Siciliano, S. and Simões-Lopes, P.C. (1996) The dwarf form of the minke whale, Balaenoptera acutorostrata Lacépède, 1804, in Brazil. Reports of the International Whaling Commission 46, 333–340.Google Scholar
Zerbini, A.N., Secchi, E.R., Siciliano, S. and Simões-Lopes, P.C. (1997) A review of the occurrence and distribution of whales of the genus Balaenoptera along the Brazilian coast. Reports of the International Whaling Commission 47, 407–417.Google Scholar
Zerbini, A.N., Secchi, E.R., Bassoi, M., Rosa, L.D., Higa, A., Sousa, L., Moreno, I.B., Möller, L.M. and Caon, G. (2004) Distribuição e abundância relativa de cetáceos na Zona Econômica Exclusiva na Região Sudeste-Sul do Brasil. Série Documentos REVIZEE–Score Sul– IO-USP, 40 pp.Google Scholar