Epidemiology and Infection

Original Papers

Gastroenteritis

Cryptosporidium spp. in calves and cows from organic and conventional dairy herds

C. SILVERLÅSa1a2 c1 and I. BLANCO-PENEDOa1

a1 Department of Clinical Sciences, Division of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

a2 Department of Animal Health and Antimicrobial Strategies, Swedish National Veterinary Institute, Uppsala, Sweden

SUMMARY

A cohort study was performed to investigate cryptosporidial prevalence and species distribution in 13 organic and 13 conventional dairy herds. Faecal samples were collected from 221 calves and 259 cows. Management routines were recorded at farm inspection and through a questionnaire. Samples were concentrated using sodium chloride flotation and cryptosporidial oocysts were detected by epifluorescence microscopy. Molecular analysis was used to determine species and subtypes. A multivariable model for factors associated with calves being Cryptosporidium spp. positive was built. Cryptosporidium spp.-positive animals were identified in all herds. Prevalences were similar in organic and conventional calves (44·7% vs. 52·3%), as well as in cows (3·1% vs. 3·8%), P > 0·05. Cryptosporidium bovis, C. ryanae and C. parvum were identified. C. ryanae was identified in a calf younger than the described prepatent period. The multivariable model included four significant variables; calf age, cleanliness of bedding, cleaning routines for group pens and farmers' attitudes towards biosecurity.

(Received November 21 2011)

(Revised April 02 2012)

(Accepted April 11 2012)

(Online publication May 08 2012)

Key words

  • Cattle;
  • Cryptosporidium ;
  • epidemiology;
  • zoonoses

Correspondence:

c1 Author for correspondence: Dr C. Silverlås, Department of Clinical Sciences, Division of Ruminant Medicine and Veterinary Epidemiology, Swedish University of Agricultural Sciences, P.O. Box 7054, SE-750 07 Uppsala, Sweden. (Email: charlotte.silverlas@slu.se)

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