Twin Research and Human Genetics

Articles

The Minnesota Center for Twin and Family Research Genome-Wide Association Study

Michael B. Millera1 c1, Saonli Basua2, Julie Cunninghama3, Eleazar Eskina4, Steven M. Malonea1, William S. Oettinga5, Nicholas Schorka6, Jae Hoon Sula4, William G. Iaconoa1 and Matt McGuea1a7

a1 Minnesota Center for Twin and Family Research, Department of Psychology, University of Minnesota, Minneapolis, MN, USA

a2 Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN, USA

a3 Department of Laboratory Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA

a4 Departments of Computer Science and Human Genetics, University of California, Los Angeles, CA, USA

a5 College of Pharmacy, Department of Experimental and Clinical Pharmacology, and the Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA

a6 Department of Molecular and Experimental Medicine, The Scripps Research Institute and The Scripps Translational Science Institute, La Jolla, CA, USA

a7 Institute of Public Health, University of Southern Denmark, Odense, Denmark

Abstract

As part of the Genes, Environment and Development Initiative, the Minnesota Center for Twin and Family Research (MCTFR) undertook a genome-wide association study, which we describe here. A total of 8,405 research participants, clustered in four-member families, have been successfully genotyped on 527,829 single nucleotide polymorphism (SNP) markers using Illumina's Human660W-Quad array. Quality control screening of samples and markers as well as SNP imputation procedures are described. We also describe methods for ancestry control and how the familial clustering of the MCTFR sample can be accounted for in the analysis using a Rapid Feasible Generalized Least Squares algorithm. The rich longitudinal MCTFR assessments provide numerous opportunities for collaboration.

(Received August 11 2012)

(Accepted August 16 2012)

Keywords:

  • GWAS;
  • rapid feasible generalized least squares;
  • Minnesota Center for Twin and Family Research
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