British Journal of Nutrition

Papers on General Nutrition

Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis

Peter J. Reedsa1, Susan M. Haya1, Patricia M. Dorwooda1 and Robert M. Palmera1

a1 Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB

Abstract

1. Young rats were offered to appetite a semi-synthetic diet either alone or containing the β2-selective agonist clenbuterol (4-amino-α[t-butylamino) methyl]-3, 5-dichlorobenzyl alcohol).

3. Male rats (starting weight 53 g) received clenbuterol at a daily oral dose of 200 pg/kg body-weight per d. Animals were slaughtered after 0, 4, 8, 11, 18, 21 and 25 d of treatment. At 4, 11, 21 and 25 d muscle protein synthesis was measured by the method of Garlick et al. (1980). Although clenbuterol increased the rate of protein and RNA accretion in gastrocnemius and soleus muscles, protein synthesis was not increased.

4. The results suggested that the drug had a rapid, perhaps direct, inhibitory effect on protein degradation. It is concluded that the growth-promoting effect of clenbuterol may be specific to muscle and that the drug may act in a novel manner which circumvents the physiological mechanisms responsible for the control of muscle growth.

(Received September 05 1985)

(Accepted February 10 1986)

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