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Effect of dietary proteins on insulin-like growth factor-1 (IGF-1) messenger ribonucleic acid content in rat liver

Published online by Cambridge University Press:  09 March 2007

Yutaka Miura
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Hisanori Kato
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
Tadashi Noguchi
Affiliation:
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan
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Abstract

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Effects of quantity and quality of dietary proteins on plasma immunoreactive insulin-like growth factor-1 (IGF-1) concentration, and content of IGF-1 mRNA in rat liver were investigated in rats. Plasma immunoreactive IGF-1 concentration in rats given a casein diet was higher than that in rats given a soya-bean-protein or protein-free diet. The IGF-1 mRNA content in liver was estimated by the Northern blot hybridization technique employing 32P-labelled rat IGF-1 complementary DNA (cDNA). At least four molecular species of IGF-1 mRNA of different molecular weight were found in rat liver. The sizes were 0·8–1·2, 2·0, 3·6–4·0 and 7·4 kb. Most of the mRNA species decreased in the livers of rats given a gluten diet (120 g gluten/kg diet) compared with rats given the casein diet. In particular, mRNA of 7·4 kb decreased markedly. When rats were fed on the protein-free diet, mRNA of all species decreased significantly. The estimated IGF-1 mRNA in the livers of rats fed on the gluten or protein-free diet was almost 0·4 of that of the rats given the casein diet. Feeding the soya-bean-protein diet did not result in a marked effect on the hepatic content of mRNA species of IGF-1. The results showed that liver IGF-1 mRNA content is sensitively regulated by quantity and nutritional quality of dietary proteins.

Type
Metabolism Effects of Diet
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

American Institute of Nutrition (1977) Report of the AIN Ad Hoc Committee on Standards for Nutritional Studies. Journal of Nutrition 107, 13401348.Google Scholar
Bolze, M. S., Reevers, R. D., Lindbeck, F. E. & Eldeers, M. J. (1985) Influence of selected amino acid deficiencies on somatomedin, growth and glycosaminoglycan metabolism in weanling rats. Journal of Nutrition 115, 782787.Google Scholar
Chomczynski, P. & Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156159.Google Scholar
D'Ercole, A. J., Stiles, A. D. & Underwood, L. E. (1984) Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proceedings of the National Academy of Sciences, U.S.A. 81, 935939.Google Scholar
Doglio, A., Dani, C., Fredrikson, G., Grimaldi, P. & Ailhaud, G. (1987) Acute regulation of insulin-like growth factor-I gene expression by growth hormone during adipose cell differentiation. EMBO Journal 6, 40114016.Google Scholar
Emler, C. A. & Schalch, D. S. (1987) Nutritionally-induced changes in hepatic insulin-like growth factor I (IGF-1) gene expression in rats. Endocrinology 120, 832834.Google Scholar
Froesch, E. R., Schmid, C., Schwander, J. & Zapf, J. (1985) Actions of insulin-like growth factors. Annual Review of Physiology 47, 443467.Google Scholar
Hoyt, E. C., Van Wyk, J. J. & Lund, P. K. (1988) Tissue and development specific regulation of a complex family of rat insulin-like growth factor-I messenger ribonucleic acids. Molecular Endocrinology 2, 10771086.Google Scholar
Isley, W. L., Underwood, L. E. & Clemmons, D. R. (1983) Dietary components that regulate serum somatomedin-C concentrations in humans. Journal of Clinical Investigation 71, 175182.Google Scholar
Kato, H., Okoshi, A., Miura, Y. & Noguchi, T. (1990a) A new cDNA clone relating to larger molecular species of rat insulin-like growth factor-I mRNA. Agricultural and Biological Chemistry 54, 15991601.Google Scholar
Kato, H., Takenaka, A., Miura, Y., Nishiyama, M. & Noguchi, T. (1990b) Evidence of introduction by molecular cloning of artificial inverted sequence at the 5' terminus of the sense strand of rat insulin-like growth factor-I cDNA. Agricultural and Biological Chemistry 54, 22252230.Google Scholar
Lund, P. K., Hoyt, E. C. & Van Wyk, J. J. (1989) The size heterogeneity of rat insulin-like growth factor-I mRNAs is due primarily to differences in the length of 3'-untranslated sequence. Molecular Endocrinology 3, 20542061.Google Scholar
Maes, M., Amand, Y., Underwood, L. E., Maiter, D. & Ketelslegers, J.-M. (1988) Decreased serum insulin-like growth factor I response to growth hormone in hypophysectomized rats fed a low protein diet: evidence for a postreceptor defect. Acta Endocrinologica 117, 320326.Google Scholar
Maiter, D., Fliesen, T., Underwood, L. E., Maes, M., Gerard, G., Davenport, M. I. & Ketelslegers, J.-M. (1989) Dietary protein restriction decreases insulin-like growth factor I independent of insulin and liver growth hormone binding. Endocrinology 124, 26042611.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (editors) (1982) Electrophoresis of RNA through gels containing formaldehyde. In Molecular Cloning. A Laboratory Manual pp. 202203. New York: Cold Spring Harbor Laboratory.Google Scholar
Moats-Staats, B. M., Brady, J. L. Jr, Underwood, L. E. & D'Ercole, A. J. (1989) Dietary protein restriction in artificially reared neonatal rats causes a reduction of insulin-like growth factor-I gene expression. Endocrinology 125, 23682374.Google Scholar
Murphy, L. J., Bell, G. I. & Friesen, H. G. (1987) Tissue distribution of insulin-like growth factor I and II messenger ribonucleic acid in the adult rat. Endocrinology 120, 12791282.Google Scholar
Phillips, L. S., Orawski, A. T. & Belosky, D. C. (1978) Somatomedin and nutrition. IV. Regulation of somatomedin activity and growth cartilage activity by quantity and composition of diet in rats. Endocrinology 103, 121127.Google Scholar
Prewitt, T. E., D'Ercole, A. J., Switzer, B. R. & Van Wyk, J. J. (1982) Relationship of serum immunoreactive somatomedin-C to dietary proteins and energy in growing rats. Journal of Nutrition 112, 144150.Google Scholar
Rinderknecht, E. & Humbel, R. E. (1978) The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. Journal of Biological Chemistry 253, 27692776.Google Scholar
Roberts, C. T. Jr, Brown, A. L., Graham, D. E., Seelig, S., Berry, S., Gabbay, K. H. & Rechler, M. M. (1986) Growth hormone regulates the abundance of insulin-like growth factor I RNA in adult rat liver. Journal of Biological Chemistry 261, 1002510028.Google Scholar
Roberts, C. T. Jr, Lasky, S. R., Lowe, W. L. Jr & LeRoith, D. (1987) Rat IGF-I cDNAs contain multiple 5'- untranslated regions. Biochemical and Biophysical Research Communications 146, 11541159.Google Scholar
Rogers, Q. R. & Harper, A. E. (1965) Amino acid diets and maximal growth in the rat. Journal of Nutrition 87, 217225.Google Scholar
Schwander, J. C., Hauri, C., Zapf, J. & Froesch, E. R. (1983) Synthesis and secretion of insulin-like growth factor and its binding protein by the perfused rat liver: Dependence on growth hormone status. Endocrinology 113, 297305.Google Scholar
Shimatsu, A. & Rotwein, P. (1987a) Mosaic evolution of the insulin-like growth factors: Organization, sequence and expression of the rat insulin-like growth factor I gene. Journal of Biological Chemisty 262, 78947900.Google Scholar
Shimatsu, A. & Rotwein, P. (1987b) Sequence of two rat insulin-like growth factor I mRNA differing within the 5'-untranslated region. Nucleic Acids Research 15, 7196.Google Scholar
Snedecor, G. W. & Cochran, W. G. (1967) One-way classifications. Analysis of variance. In Statistical Methods, 6th ed, pp. 271273. Ames, Iowa: Iowa State University Press.Google Scholar
Straus, D. S. & Takemoto, C. D. (1990) Effect of fasting on insulin-like growth factor-I (IGF-I) and growth hormone receptor mRNA levels and IGF-I gene transcription in rat liver. Molecular Endocrinology 4, 91100.Google Scholar
Takahashi, S., Kajikawa, M., Umezawa, T., Takahashi, S.-I., Kato, H., Miura, Y., Nam, T. J., Noguchi, T. & Naito, H. (1990) Effect of dietary proteins on the plasma immunoreactive insulin-like growth factor-1/somatomedin C concentration in the rat. British Journal of Nutrition 63, 521534.Google Scholar
Van Wyk, J. J. (1984) The somatomedins: Biological actions and physiologic control mechanisms. In Hormonal Proteins and Peptides, vol. XII, pp. 81125. New York and London: Academic Press.Google Scholar