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Determination of leucine metabolism and protein turnover in sheep, using gas–liquid chromatography–mass spectrometry

Published online by Cambridge University Press:  09 March 2007

C. R. Krishnamurti  and
S. M. Janssens
C. R. Krishnamurti
Affiliation:
Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada
S. M. Janssens
Affiliation:
Department of Animal Science, University of British Columbia, Vancouver, B.C. V6T 2A2, Canada
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Abstract

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1. Whole-body protein synthetic rates in non-pregnant ewes were determined by the continuous infusion of L-[15N]- and [1-13C]leucine and measuring the plasma enrichment of leucine, α-ketoisocaproate (α-KIC) and expired carbon dioxide by gas–liquid chromatography–mass spectrometry.

2. The mean whole-body protein synthesis estimated from plasma leucine flux corrected for oxidation was 5·38 (SE 0·54) g/kg per d.

3. Under the conditions of the present study leucine oxidation was 0·323 (SE 0·067) mmol/kg per d and accounted for 10·71 (SE 2·26) % of plasma [13C]leucine flux. Deamination of leucine was 0·55 (SE 0·035) mmol/kg per d and accounted for approximately 17% of plasma [15N]leucine flux.

4. The rate of α-KIC reamination to leucine, calculated by subtracting 13C flux from 15N flux, was 0·228 (SE 0·101) mmol/kg per d.

5. The rate of whole-body protein degradation was 4·49 (SE 0·54) g/kg per d and there was a net protein gain of 0·89 (SE 0·21) g/kg per d.

Type
General Nutrition papers
Information
British Journal of Nutrition , Volume 59 , Issue 1 , January 1988 , pp. 155 - 164
Copyright
Copyright © The Nutrition Society 1988

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