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Detection and Damage-analysis of Bio-Particles and for Safety-Evaluation of Plasma-treated water using DNA-manipulation

Published online by Cambridge University Press:  23 May 2012

Akira Mizuno ,
Hachiro Yasuda ,
Hirofumi Kurita  and
Kazunori Takashima
Akira Mizuno
Affiliation:
Dept. Environmental and Life Sciences, Toyohashi University of Technology, Hibarigaoka 1-1, Tenpaku-cho, Toyohashi, 441-8580 Japan
Hachiro Yasuda
Affiliation:
Dept. Environmental and Life Sciences, Toyohashi University of Technology, Hibarigaoka 1-1, Tenpaku-cho, Toyohashi, 441-8580 Japan
Hirofumi Kurita
Affiliation:
Dept. Environmental and Life Sciences, Toyohashi University of Technology, Hibarigaoka 1-1, Tenpaku-cho, Toyohashi, 441-8580 Japan
Kazunori Takashima
Affiliation:
Dept. Environmental and Life Sciences, Toyohashi University of Technology, Hibarigaoka 1-1, Tenpaku-cho, Toyohashi, 441-8580 Japan
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Abstract

Effect of non-thermal plasma (NTP) on bio-particles has been studied using Bacillus subtilis (B. subtilis), Escherichia coli (E. coli) and bacteriophages. NTP has been used, and states of different biological components were monitored during the course of the exposure. Analysis of green fluorescent protein (GFP), introduced into E.coli cells proved that NTP causes a prominent protein damages without cutting peptide bonds. We have developed a biological assay which evaluates in vivo DNA damage of the bacteriophages. Different doses of the plasma were applied to wet state of λ phages. From the plasma-exposed λ phages, DNA was purified and subjected to in vitro DNA packaging reactions. The re-packaged phages consist of the DNA from discharged phages and brand-new coat proteins. Survival curves of the re-packaged phages showed extremely large D value (D = 25 s) compared to the previous D value (D = 3 s) from the discharged phages. The results indicate that DNA damage hardly contributed to the inactivation, and the damage in coat proteins is responsible for inactivation of the phages. We also report a single-molecule-based analysis of strand breakages on large DNA molecules induced by the plasma exposure. Single-molecule observation of DNA that involved molecular combing was used to measure the length of individual DNA molecules. The measured DNA length showed that plasma exposure caused a marked change in length of DNA molecules. The rate of plasmainduced strand breakage on large random-coiled DNA molecules was determined using a simple mathematical model. The measured rate shows good relation with the plasma exposure time, and could be used for safety evaluation of the plasma treated water.

Keywords

biologicalbiomedicalnanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1469: Symposium WW – Plasma Processing and Diagnostics for Life Sciences. , 2012 , mrss12-1469-ww04-05
Copyright
Copyright © Materials Research Society 2012

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