a1 USDA-ARS †, Crop Production and Pest Control Unit, 170 South University Street, West Lafayette, IN 47907, USA
a2 Purdue University, Department of Entomology, 901 West State Street, West Lafayette, IN 47907, USA
a3 Agricultural Research Organization, Gilat Research Center, D.N. Negev, 85280, Israel
a4 CSKHPKV, Palampur, HP, 176062, India
Samples of a dipteran pest of wheat were tested to confirm identity, describe local populations and suggest the use of deploying resistance (R) genes in wheat cultivars for control of Mayetiola destructor, Hessian fly (HF). Morphological evaluation of adults and a free-choice oviposition preference test documenting that females overwhelmingly preferred to oviposit on wheat instead of barley supported they were HF. Using the cytochrome c oxidase subunit I (coxI), the Barcoding Region, nine haplotypes were revealed. Two were found only in the Israeli collections and averaged 3% sequence divergence compared to the other seven haplotypes found in the United States, Israel and Syria. In evaluations of virulence, the Israeli HF in culture was virulent to 11 of the 19 (R) genes tested, and complementation analysis documented that, for four of the R genes tested, the Israeli HF shared loci for virulence with HF from the United States. Levels of HF infestation at seven Israeli fields were at least at the 5–8% level, which historically has indicated a significant yield loss. Microsatellite genotyping of the five HF collections from Israel revealed mixed populations in Israel that are distinctly separate from the single population in Syria.
(Accepted March 11 2012)
(Online publication May 08 2012)
† Mention of a commercial or proprietary product does not constitute an endorsement by the USDA.