a1 Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 80309 CO, USA
a2 Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Moscow 119991, Russia
a3 National Research Center for Hematology, Moscow 117513, Russia
a4 Physics Department, Lomonosov Moscow State University, Moscow 119991, Russia
Mitosis is the process by which eukaryotic cells organize and segregate their chromosomes in preparation for cell division. It is accomplished by a cellular machine composed largely of microtubules (MTs) and their associated proteins. This article reviews literature on mitosis from a biophysical point of view, drawing attention to the assembly and motility processes required to do this complex job with precision. Work from both the recent and the older literature is integrated into a description of relevant biological events and the experiments that probe their mechanisms. Theoretical work on specific subprocesses is also reviewed. Our goal is to provide a document that will expose biophysicists to the fascination of this quite amazing process and provide them with a good background from which they can pursue their own research interests in the subject.