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Haplosporidium raabei n. sp. (Haplosporidia): a parasite of zebra mussels, Dreissena polymorpha (Pallas, 1771)

Published online by Cambridge University Press:  05 January 2012

D. P. MOLLOY ,
L. GIAMBÉRINI ,
N. A. STOKES ,
E. M. BURRESON  and
M. A. OVCHARENKO
D. P. MOLLOY*
Affiliation:
Division of Research and Collections, New York State Museum, Albany, NY 12230, USA
L. GIAMBÉRINI
Affiliation:
Université Paul Verlaine – Metz, Laboratoire des Interactions, Ecotoxicologie, Biodiversité, Ecosystèmes (LIEBE), CNRS UMR 7146, Campus Bridoux, Rue du Général Delestraint, F-57070 Metz, France
N. A. STOKES
Affiliation:
Virginia Institute of Marine Science, College of William & Mary, Gloucester Point, P.O. Box 1346, Virginia 23062, USA
E. M. BURRESON
Affiliation:
Virginia Institute of Marine Science, College of William & Mary, Gloucester Point, P.O. Box 1346, Virginia 23062, USA
M. A. OVCHARENKO
Affiliation:
Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland Pomeranian Akademy, Arcishewski str. 22b, 76-200, Słupsk, Poland
*
*Corresponding author: Present address: Department of Biological Sciences, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA. Tel: +1 518 677 8245. Fax: +1 518 677 5236. E-mail: dmolloy@albany.edu
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Summary

Extensive connective tissue lysis is a common outcome of haplosporidian infection. Although such infections in marine invertebrates are well documented, they are relatively rarely observed in freshwater invertebrates. Herein, we report a field study using a comprehensive series of methodologies (histology, dissection, electron microscopy, gene sequence analysis, and molecular phylogenetics) to investigate the morphology, taxonomy, systematics, geographical distribution, pathogenicity, and seasonal and annual prevalence of a haplosporidian observed in zebra mussels, Dreissena polymorpha. Based on its genetic sequence, morphology, and host, we describe Haplosporidium raabei n. sp. from D. polymorpha – the first haplosporidian species from a freshwater bivalve. Haplosporidium raabei is rare as we observed it in histological sections in only 0·7% of the zebra mussels collected from 43 water bodies across 11 European countries and in none that were collected from 10 water bodies in the United States. In contrast to its low prevalences, disease intensities were quite high with 79·5% of infections advanced to sporogenesis.

Keywords

Haplosporidium raabei n. sp.HaplosporidiaDreissena polymorphaphylogenysmall subunit ribosomal DNA
Type
Research Article
Information
Parasitology , Volume 139 , Issue 4 , April 2012 , pp. 463 - 477
Copyright
Copyright © Cambridge University Press 2012

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Supplementary material: PDF

Molloy Supplementary Data

Sample collection data for histological examination of zebra mussels for H. raabei

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