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Cholesterol Biosensor Based on Nanodiamond-Polypyrrole Conducting Nanocomposite Membrane

Published online by Cambridge University Press:  27 February 2012

Pedro Villalba ,
Punya Basnayaka ,
Mikhail Ladanov ,
Beverly Liriano ,
Manoj Ram ,
Subhra Mohapatra  and
Ashok Kumar
Pedro Villalba*
Affiliation:
Chemical and Biomedical Engineering, University of South Florida, Tampa, FL 33613, United States. Nanotechnology Research & Education Center, University of South Florida, Tampa, FL 33613 United States. College of Medicine, Universidad del Norte, Barranquilla, Colombia.
Punya Basnayaka
Affiliation:
Nanotechnology Research & Education Center, University of South Florida, Tampa, FL 33613 United States. Mechanical Engineering, University of South Florida, Tampa, FL 33613, United States.
Mikhail Ladanov
Affiliation:
Nanotechnology Research & Education Center, University of South Florida, Tampa, FL 33613 United States.
Beverly Liriano
Affiliation:
Mechanical Engineering, University of South Florida, Tampa, FL 33613, United States.
Manoj Ram
Affiliation:
Nanotechnology Research & Education Center, University of South Florida, Tampa, FL 33613 United States. Mechanical Engineering, University of South Florida, Tampa, FL 33613, United States.
Subhra Mohapatra
Affiliation:
Department of Molecular Medicine, University of South Florida, Tampa, FL 33613, United States.
Ashok Kumar
Affiliation:
Nanotechnology Research & Education Center, University of South Florida, Tampa, FL 33613 United States. Mechanical Engineering, University of South Florida, Tampa, FL 33613, United States.
*
*Corresponding author
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Abstract

In this work we present the synthesis, characterization and application of the nanodiamond- polypyrrole nanocomposite films as electrochemical active electrode for sensing cholesterol. Cholesterol oxidase and esterase have been covalently attached to the surface of the films. Results showed that the inclusion of nanodiamond to the polymeric matrix increment the surface area and provide carboxylic groups capable to perform reaction with the amide groups present in the enzymes. Further, the sensitivity of the electrode to the cholesterol content have been examined.

Keywords

diamondpolymersensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1414: Symposium HH – Bioelectronics–Materials, Properties and Applications , 2012 , mrsf11-1414-hh02-03
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

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