Parasitology

Research Article

Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status

AILEEN M. HALLIDAYa1, F. ALEX LAINSONa1, RAJA YAGAa1, NEIL F. INGLISa1, STEPHEN BRIDGETTa2, MINTU NATHa3 and DAVE P. KNOXa1 c1

a1 Moredun Research Institute, Bush Loan, Penicuik EH26 0PZ, UK

a2 The GenePool, Ashworth Laboratories, The King's Buildings, The University of Edinburgh, Edinburgh EH9 3JT, UK

a3 Biomathematics and Statistics Scotland, James Clerk Maxwell Building, The King's Buildings, Edinburgh EH9 3JZ, UK

SUMMARY

The aim of this study was to elucidate transcriptional changes in the parasitic nematode Teladorsagia circumcincta upon encountering either naïve or immune ovine hosts. Pools of 100 000 exsheathed 3rd- stage T. circumcincta larvae were exposed in vitro to either an immune or naïve ovine abomasal environment, RNA was extracted from the larvae and sequenced using the Roche 454 platform. Each sample produced approximately 82 000 reads that assembled to give approximately 5500 Isotigs (contigs). The two sequence datasets were clustered together to give a total of 6969 clusters of which 18 were differentially expressed (P<0·001) between the two groups. Clusters with a predominance of reads in larvae exposed to the immune abomasal environment encoded homologues of peptidyl-glycine alpha-amidating monooxygenase, heat shock-protein 16-2 and IDA-1, a tyrosine phosphatase-like receptor protein. Clusters with a predominance of reads in the naïve environment encoded homologues of cytochrome b, EGg Laying defective family member 21 and NADH dehydrogenase subunit 5. Gene ontology analyses indicated that larvae exposed to the immune environment showed an increase in expression of genes involved in ‘carbon utilization’, ‘response to stimulus’ and ‘developmental process’. These data suggest that T. circumcincta modulates gene expression in response to the immune status of the host.

(Received August 03 2011)

(Revised September 05 2011)

(Revised October 07 2011)

(Accepted October 10 2011)

(Online publication January 05 2012)

Correspondence:

c1 Corresponding author: Moredun Research Institute, Bush Loan, Penicuik EH26 0PZ, UK. Tel: +44 (0)131 445 5111. Fax: +44 (0)131 445 6111. E-mail: dave.knox@moredun.ac.uk

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