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Isolation and culture of Sphaerothecum destruens from Sunbleak (Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon (Salmo salar)

Published online by Cambridge University Press:  08 February 2012

RICHARD K. PALEY ,
DEMETRA ANDREOU ,
KELLY S. BATEMAN  and
STEPHEN W. FEIST
RICHARD K. PALEY*
Affiliation:
Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK
DEMETRA ANDREOU
Affiliation:
School of Conservation Ecology and Environmental Change, Bournemouth University, Christchurch House, Talbot Campus, Poole, Dorset BH12 5BB, UK
KELLY S. BATEMAN
Affiliation:
Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK
STEPHEN W. FEIST
Affiliation:
Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK
*
*Corresponding author: Centre for Environment Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. Tel: +44 (0)1305 206640. Fax: +44 (0)1305 206601. E-mail: richard.paley@cefas.co.uk
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Summary

The sunbleak (Leucaspius delineatus), a cyprinid fish native to continental Europe and now established in the UK, is experiencing population decline which appears to be linked to the spread of the invasive Asian cyprinid (Pseudorasbora parva). A population of sunbleak in the UK has previously been identified as infected with S. destruens at low prevalence. Because Sphaerothaecum destruens has, on occasion, caused severe disease in cultured and wild salmonids the aim of this work was to establish laboratory cultures of S. destruens from sunbleak in the UK and use these cultures in challenge experiments to determine if the UK isolate of S. destruens from cyprinid species is a potential threat to Atlantic salmon (Salmo salar). The first isolation and culture of S. destruens in the UK and from a cyprinid species is described. Cultured S. destruens spores from sunbleak are infective to EPC, CHSE and FHM cells, replicating most rapidly in FHM and EPC cells. Spores can be induced to zoosporulate in water forming motile, uni-flagellated zoospores. Challenge experiments indicated the spores are able to replicate and disperse in Atlantic salmon and are associated with increased mortality (up to 90%) when injected intraperitonealy.

Keywords

rosette agentcyprinids18SIP injectionbath immersionsporeszoospores
Type
Research Article
Information
Parasitology , Volume 139 , Issue 7 , June 2012 , pp. 904 - 914
Copyright
Copyright © Cambridge University Press 2012

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