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NTPDase activity in lymphocytes of rats infected by Trypanosoma evansi

Published online by Cambridge University Press:  05 January 2012

CAMILA B. OLIVEIRA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
ALEKSANDRO S. DA SILVA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
VIVIANE C. G. SOUZA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
MARCIO M. COSTA
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
JEANDRE A. S. JAQUES
Affiliation:
Department of Chemistry, Universidade Federal de Santa Maria, Brazil
DANIELA B. R. LEAL
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
SONIA T. A. LOPES
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
*
*Corresponding author: Departamento de Microbiologia e Parasitologia da UFSM. Faixa de Camobi – Km 9, Campus Universitário, Santa Maria – RS, 97105-900, Prédio 20, Sala 4232, Brasil. Fax: +55 55 3220 8958. E-mail: camilabelmontevet@yahoo.com.br

Summary

Trypanosoma evansi is the aetiological agent of trypanosomosis in domestic animals. In this pathology, an inflammatory response can be observed and, as a consequence, the increase of extracellular adenine nucleotides such as ATP. These nucleotide concentrations are regulated by ectoenzymes such as NTPDase (EC 3.6.1.5, CD39), which catalyses the hydrolysis of ATP and ADP into AMP. In this study, the activity of NTPDase in lymphocytes of rats experimentally infected with T. evansi was evaluated. The animals were inoculated with the parasite and monitored by blood smear on a daily basis. The animals were then were divided into 4 groups according to the degree of parasitaemia and period of infection. The blood collections for enzyme analysis and lymphocyte count were performed on the 3rd (beginning of infection), 5th (acute infection) and 15th (chronic infection) days post-infection (p.i.). The control group was composed of non-infected animals. In the infected group a decrease in ATP hydrolysis (36%) was observed on the 3rd day p.i. and a decrease in ADP hydrolysis (62%) was observed on the 5th day p.i. when compared to the control. On the 15th day p.i., an increase in ATP (94%) and ADP (50%) hydrolysis was observed in the infected group. Considering these data it is suggested that NTPDase activity is altered on the surface of lymphocytes of rats infected with T. evansi at different time-points of infection.

Type
Research Article
Creative Commons
This is a work of the U.S. Government and is not subject to copyright protection in the United States.
Copyright
Copyright © Cambridge University Press 2012. This is a work of the U.S. Government and is not subject to copyright protection in the United States.

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References

REFERENCES

Adnolfi, E., Pizzirani, C., Idzko, M., Panther, E., Norgauer, J., Di Virgílio, F. and Ferrari, D. (2005). P2X7 receptor: death or life? Purinergic Signalling 1, 219227.CrossRefGoogle Scholar
Assoku, R. K. (1975). Immunological studies of the mechanism of anaemia in experimental Trypanosoma evansi infection in rats. International Journal for Parasitology 5, 137145.CrossRefGoogle ScholarPubMed
Bach, B. C., Leal, D. B. R., Ruchel, J. B., Souza, V. C. G., Maboni, G., Dal Pozzo, M., Schlemmer, K. B., Alves, S. H. and Santurio, J. M. (2011). Immunotherapy for pythiosis: Effect on NTPDase activity in lymphocytes of an experimental model. Biomedicine & Pharmacotherapy 64, 718722.CrossRefGoogle Scholar
Baral, T. N., Baetselier, P., Brombachier, F. and Magez, S. (2007). Control of Trypanosoma evansi infection is IgM mediated and does not require a type inflammatory response. Journal of Infectious Diseases 195, 15131520.CrossRefGoogle Scholar
Batista, J. S. (2006). Infecção experimental por Trypanosoma vivax em ovinos. Pesquisa Veterinária Brasileira 26, 3137.CrossRefGoogle Scholar
Bergmeyer, H. U. (1983). Methods of Enzymatic Analysis, 3rd Edn.Verlag Chemie, Weinheim, Germany.Google Scholar
Böyum, A. (1968). Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation and of granulocytes by combining centrifugation and sedimentation at 1 g. Scandinavian Journal of Clinical & Laboratory Investigation 97, 7789.Google ScholarPubMed
Bradford, M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein – dye binding. Analytical Biochemistry 72, 248254.CrossRefGoogle Scholar
Bush, B. M. (2004). Interpretação de Resultados Laboratoriais para Clínicos de Pequenos Animais, 1st Edn.Roca, São Paulo, Brasil.Google Scholar
Chan, K., Delfert, D. and Junger, K. D. (1986). A direct colorimetricassay for the Ca2+ – ATPase activity. Analytical Biochemistry 157, 375380.CrossRefGoogle Scholar
Colpo, C. B., Monteiro, S. G. and Stainki, D. R. (2005). Natural infection by Trypanosoma evansi in dogs. Ciência Rural 35, 717719.CrossRefGoogle Scholar
Da Silva, A. S., Belle, L., Bitencourt, P. E. R., Souza, V. C. G., Costa, M. M., Oliveira, C. B., Jaques, J. A., Moretto, M. B., Mazzanti, C. M., Leal, D. B. R., Lopes, S. T. A. and Monteiro, S. G. (2011). Activity of the enzyme adenosine deaminase in serum, erythrocytes and lymphocytes of rats infected with Trypanosoma evansi. Parasitology 138, 201208.CrossRefGoogle ScholarPubMed
Da Silva, A. S., Doyle, R. L. and Monteiro, S. G. (2006). Métodos de contenção e confecção de esfregaço sanguíneo para pesquisa de hemoparasitas em ratos e camundongos. Revista da Faculdade de Medicina Veterinária, Zootecnia e Agronomia 13, 8387.Google Scholar
Dombrowski, K. E., Bewer, K. A. and Kapp, J. A. (1998). Ecto-ATPase: An activation marker necessary for effector cell function. Immunological Reviews 161, 111118.CrossRefGoogle ScholarPubMed
Fillippini, A., Taffs, R. E., Agui, T. and Sitkovsky, M. V. (1990). Ecto-ATPase activity in cytolytic T-lymphocytes. Protection from the cytolytic effects of extracellular ATP. Journal of Biological Chemistry 265, 334340.CrossRefGoogle Scholar
Hoare, C. A. (1972). The Trypanomoses of Mammals: a Zoological Monograph. Blackwell Scientific Publications, Oxford, UK.Google Scholar
Kaczmarek, E., Koziak, K., Sévigny, J., Siegel, J. B., Anrather, J. and Beaudoin, A. R. (1996). Identification and characterization of CD39 vascular ATP diphosphohydrolase. The Journal of Biological Chemistry 271, 3311633122.CrossRefGoogle ScholarPubMed
Kumar, V. and Sharma, A. (2009). Adenosine: an endogenous modulator of innate immune system with therapeutic potential. European Journal Pharmacology 616, 715.CrossRefGoogle ScholarPubMed
Langston, H. P., Ke, Y., Dombrowsky, K. E., Gewirtz, A. T. and Kapp, J. (2003). Secretion of IL-2 and IFN-γ, but not IL-4, by antigen-specific T cells requires extracellular ATP. The Journal of Immunology 170, 29622970.CrossRefGoogle Scholar
Leal, D. B. R., Streher, C. A., Neu, T. N., Bittencourt, F. P., Leal, C. A. M., Silva, J. E. P., Morsch, V. M. and Schetinger, M. R. C. (2005). Characterization of NTPDase (NTPDase1; ecto-apyrase; ecto- diphosphohydrolase; CD39; E.C. 3.6.1.5) activity in human lymphocytes. Biochimica et Biophysica Acta 1721, 911.CrossRefGoogle Scholar
Marques, L. C. (2000). Experimental infection with Trypanosoma evansi in horses: clinical and haematological observations. Revista Brasileira de Parasitologia Veterinária 9, 1115.Google Scholar
Masfield, J. M. and Bagasra, O. (1978). Lymphocyte function in experimental African trypanosomiasis. I. B-cell responses to helper T cell-independent and dependent antigens. Journal of Immunology 120, 759765.Google Scholar
Neitz, W. O. and McCully, R. M. (1971). Clinicopathological study of experimental Trypanosoma brucei infections in horses. 1. Development of clinical recognizable nervous symptoms in Nagana-infected horses treated with subcurative doses of Antrypol and berenil. Onderstepoort. Journal Veterinary Research 38, 127140.Google Scholar
Ngeranwa, J. J. (1993). Pathogenesis of Trypanosoma evansi in small east African goats. Research in Veterinary Science 54, 283289.CrossRefGoogle ScholarPubMed
Oliveira, C. B., Da Silva, A. S., Vargas, L. B., Bitencourt, P. E. R., Souza, V. C. G., Costa, M. M., Leal, C. A. M., Moretto, M. B., Leal, D. B. R., Lopes, S. T. A. and Monteiro, S. G. (2011). Activities of adenine nucleotide and nucleoside degradation enzymes in platelets of rats infected by Trypanosoma evansi. Veterinary Parasitology 178, 914.CrossRefGoogle ScholarPubMed
Ralevic, V. and Burnstock, G. (1998). Receptores for purines and pyrimidines. Pharmacological Reviews 50, 413492.Google Scholar
Rodrigues, A. (2005). Outbreaks of trypanosomiasis in horses by Trypanosoma evansi in the state of Rio Grande do Sul, Brazil: epidemiological, clinical, hematological, and pathological aspects. Pesquisa Veterinária Brasileira 25, 239249.CrossRefGoogle Scholar
Sharma, D. K. (2000). Haematological changes in experimental trypanosomiasis in Barbari goats. Small Ruminant Research 38, 145149.CrossRefGoogle Scholar
Trautmann, A. (2009). Extracellular ATP in the immune system: more than just a “danger signal”. Science Signaling 2, 6.CrossRefGoogle ScholarPubMed
Woo, P. T. K. (1977). Salivarian trypanosomes producing disease in livestock outside of sub-Saharan África. In Parasitic Protozoa, Vol. 1 (Kreier, J. P.), pp. 270295. Academic Press, New York, USA.Google Scholar
Wolkmer, P., Silva, A. S., Carngelutti, J. F., Costa, M. M., Traesel, C., Lopes, S. T. A. and Monteiro, S. G. (2007). Resposta eritropoética de ratos em diferentes de graus de parasitemia por Trypanosoma evansi. Ciência Rural 37, 16821687.CrossRefGoogle Scholar
Zimmermann, H. (1996). Biochemistry, localization and functional roles of ecto-nucleotidases in the nervous system. Progress in Neurobiology 49, 589618.CrossRefGoogle ScholarPubMed