Parasitology

Research Article

NTPDase activity in lymphocytes of rats infected by Trypanosoma evansi

CAMILA B. OLIVEIRAa1 c1, ALEKSANDRO S. DA SILVAa1, VIVIANE C. G. SOUZAa1, MARCIO M. COSTAa2, JEANDRE A. S. JAQUESa3, DANIELA B. R. LEALa1, SONIA T. A. LOPESa2 and SILVIA G. MONTEIROa1

a1 Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil

a2 Department of Small Animals, Universidade Federal de Santa Maria, Brazil

a3 Department of Chemistry, Universidade Federal de Santa Maria, Brazil

SUMMARY

Trypanosoma evansi is the aetiological agent of trypanosomosis in domestic animals. In this pathology, an inflammatory response can be observed and, as a consequence, the increase of extracellular adenine nucleotides such as ATP. These nucleotide concentrations are regulated by ectoenzymes such as NTPDase (EC 3.6.1.5, CD39), which catalyses the hydrolysis of ATP and ADP into AMP. In this study, the activity of NTPDase in lymphocytes of rats experimentally infected with T. evansi was evaluated. The animals were inoculated with the parasite and monitored by blood smear on a daily basis. The animals were then were divided into 4 groups according to the degree of parasitaemia and period of infection. The blood collections for enzyme analysis and lymphocyte count were performed on the 3rd (beginning of infection), 5th (acute infection) and 15th (chronic infection) days post-infection (p.i.). The control group was composed of non-infected animals. In the infected group a decrease in ATP hydrolysis (36%) was observed on the 3rd day p.i. and a decrease in ADP hydrolysis (62%) was observed on the 5th day p.i. when compared to the control. On the 15th day p.i., an increase in ATP (94%) and ADP (50%) hydrolysis was observed in the infected group. Considering these data it is suggested that NTPDase activity is altered on the surface of lymphocytes of rats infected with T. evansi at different time-points of infection.

(Received July 04 2011)

(Revised September 16 2011)

(Accepted September 18 2011)

(Online publication January 05 2012)

Correspondence:

c1 Corresponding author: Departamento de Microbiologia e Parasitologia da UFSM. Faixa de Camobi – Km 9, Campus Universitário, Santa Maria – RS, 97105-900, Prédio 20, Sala 4232, Brasil. Fax: +55 55 3220 8958. E-mail: camilabelmontevet@yahoo.com.br

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