Parasitology
- Parasitology / Volume 139 / Issue 01 / January 2012, pp 37-44
- Copyright © Cambridge University Press 2012. The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/2.5/>. The written permission of Cambridge University Press must be obtained for commercial re-use.
- DOI: http://dx.doi.org/10.1017/S0031182011001697 (About DOI), Published online: 04 January 2012
- OPEN ACCESS
Research Article
Morphological, biological and molecular characterization of three strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sordida (Stal) 1859 (Hemiptera, Reduviidae) and a domestic cat
ALINE RIMOLDIa1, RENATA TOMÉ ALVESa2, DANIELA LUZ AMBRÓSIOa2, MARIA ZENAIDE TITA FERNANDESa2, ISABEL MARTINEZa2, RENATO FREITAS DE ARAÚJOa3, REGINA MARIA BARRETO CICARELLIa2 and JOÃO ARISTEU DA ROSAa2 c1
a1 Instituto de Biologia Universidade Estadual de Campinas, Unicamp – Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255 Campinas SP, CEP 13083-862, Brasil
a2 Universidade Estadual Júlio de Mesquita Filho, UNESP Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Ciências Biológicas, CEP 14801-902, Brasil
a3 Secretaria de Saúde do Estado da Bahia, SESAB, Brasil
SUMMARY
A study was conducted of the biological, morphological and molecular characters of 3 strains of Trypanosoma cruzi (SI5, SI8 and SIGR3) isolated from specimens of Triatoma sordida collected in Santo Inácio and a domestic cat. In order to carry out the study, the following parameters were evaluated: pre-patent period, parasitaemia curves, morphology of the parasites, mortality rates, histopathological lesions and molecular typing. The strains presented variable pre-patent periods, low parasitaemia and no animal mortality. The morphological study of trypomastigotes showed a predominance of intermediate-width and short-length forms, as well as low nuclear index. Epimastigotes presented a low nuclear index, intermediate-width forms in strains SI5 and SI8, and large-width forms in SIGR3. A shorter length could be noted in strains SI8 and SIGR3, whereas SI5 displayed an intermediate length. The histopathological study did not detect amastigote nests in tissues. The amplification of the divergent domain of 24Sα rRNA, HSP60 and GPI genes of strains SI5, SI8 and SIGR3 classified the 3 strains into Group II. Biological parameters made it possible to classify the strains isolated in Santo Inácio (BA) into Biodeme III, Zymodeme 1 and Group II of T. cruzi.
(Received May 06 2011)
(Revised July 06 2011)
(Revised August 12 2011)
(Accepted August 22 2011)
(Online publication January 04 2012)
Key words
Correspondence:
c1 Corresponding author: Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara/Jau, Km1, CEP: 14801-902, Araraquara, SP, Brasil. Tel: +55 16 33016943. Fax: +55 16 33016940. E-mail: rosaja@fcfar.unesp.br
