Parasitology

Research Article

Involvement of sulfated glycosaminoglycans on the development and attachment of Trypanosoma cruzi to the luminal midgut surface in the vector, Rhodnius prolixus

MARCELO S. GONZALEZa1a6 c1, LUIZ-CLAUDIO F. SILVAa2a3, J. M. ALBUQUERQUE-CUNHAa4, NADIR F. S. NOGUEIRAa5, DÉBORA P. MATTOSa1, DANIELE P. CASTROa4a6, PATRICIA AZAMBUJAa4a6 and ELOI S. GARCIAa4a6

a1 Laboratório de Biologia de Insetos, Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense (UFF), Morro do Valonguinho s/n – Centro –Niterói, RJ, 24001-970, Brazil

a2 Programa de Glicobiologia, Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, CEP 21941-590, Brazil

a3 Laboratório de Interações Celulares, Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil

a4 Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil

a5 Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacases, RJ, 28015-620, Brazil

a6 Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Brazil

SUMMARY

In the present study, we investigated the involvement of sulfated glycosaminoglycans in both the in vivo development and adhesion of T. cruzi epimastigotes to the luminal surface of the digestive tract of the insect vector, Rhodnius prolixus. Pre-incubation of T. cruzi, Dm 28c epimastigotes with heparin, chondroitin 4-sulfate, chondroitin 6-sulfate or protamine chloridrate inhibited in vitro attachment of parasites to the insect midgut. Enzymatic removal of heparan sulfate moieties by heparinase I or of chondroitin sulfate moieties by chondroitinase AC from the insect posterior midgut abolished epimastigote attachment in vitro. These treatments also reduced the labelling of anionic sites exposed at the luminal surface of the perimicrovillar membranes in the triatomine midgut epithelial cells. Inclusion of chondroitin 4-sulfate or chondroitin 6-sulfate and to a lesser extent, heparin, in the T. cruzi-infected bloodmeal inhibited the establishment of parasites in R. prolixus. These observations indicate that sulfated glycosaminoglycans are one of the determinants for both adhesion of the T. cruzi epimastigotes to the posterior midgut epithelial cells of the triatomine and the parasite infection in the insect vector, R. prolixus.

(Received March 18 2011)

(Revised May 28 2011)

(Revised June 30 2011)

(Revised August 10 2011)

(Accepted August 12 2011)

(Online publication September 09 2011)

Correspondence:

c1 Corresponding author: Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense, Morro do Valonguinho s/n° Centro –Niterói, RJ, 24001-970, Brazil. Tel: +55 2126292285. Fax: +55 2126292376. E-mail: msgonzalez@id.uff.br

Footnotes

† L. C. F. S. and M. S. G. have participated equally in this work.

Metrics