Development of High-Throughput, High-Resolution 3D Reconstruction of Large-Volume Biological Tissue Using Automated Tape Collection Ultramicrotomy and Scanning Electron Microscopy

R Schalek; N Kasthuri; K Hayworth; D Berger; J Tapia; J Morgan; S Turaga; E Fagerholm; H Seung; J Lichtman

doi:10.1017/S1431927611005708

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Hayworth, Kenneth J. Morgan, Josh L. Schalek, Richard Berger, Daniel R. Hildebrand, David G. C. and Lichtman, Jeff W. 2014. Imaging ATUM ultrathin section libraries with WaferMapper: a multi-scale approach to EM reconstruction of neural circuits. Frontiers in Neural Circuits, Vol. 8, Issue. ,

Smolla, Marco Ruchty, Markus Nagel, Manuel and Kleineidam, Christoph J. 2014. Clearing pigmented insect cuticle to investigate small insects' organs in situ using confocal laser-scanning microscopy (CLSM). Arthropod Structure & Development, Vol. 43, Issue. 2, p. 175.

Peddie, Christopher J. Liv, Nalan Hoogenboom, Jacob P. and Collinson, Lucy M. 2014. Correlative Light and Electron Microscopy II. Vol. 124, Issue. , p. 363.

Miranda, Kildare Girard‐Dias, Wendell Attias, Marcia de Souza, Wanderley and Ramos, Isabela 2015. Three dimensional reconstruction by electron microscopy in the life sciences: An introduction for cell and tissue biologists. Molecular Reproduction and Development, Vol. 82, Issue. 7-8, p. 530.

Miyazaki, Naoyuki and Murata, Kazuyoshi 2015. 3-D subcellular morphological analyses by Serial Block-Face SEM (SBF-SEM). PLANT MORPHOLOGY, Vol. 27, Issue. 1, p. 9.

Clode, Peta L. Koh, Wan H. and Thompson, R.C. Andrew 2015. Life without a Host Cell: What is Cryptosporidium ?. Trends in Parasitology, Vol. 31, Issue. 12, p. 614.

Eberle, Anna Lena Selchow, Olaf Thaler, Marlene Zeidler, Dirk and Kirmse, Robert 2015. Mission (im)possible – mapping the brain becomes a reality. Microscopy, Vol. 64, Issue. 1, p. 45.

Satoh, Keita Takanami, Keiko Murata, Kazuyoshi Kawata, Mitsuhiro Sakamoto, Tatsuya and Sakamoto, Hirotaka 2015. Effective synaptome analysis of itch-mediating neurons in the spinal cord: A novel immunohistochemical methodology using high-voltage electron microscopy. Neuroscience Letters, Vol. 599, Issue. , p. 86.

WANNER, A. A. KIRSCHMANN, M. A. and GENOUD, C. 2015. Challenges of microtome‐based serial block‐face scanning electron microscopy in neuroscience. Journal of Microscopy, Vol. 259, Issue. 2, p. 137.

Sueters ‐ Di Meo, Josey Liv, Nalan and Hoogenboom, Jacob P. 2016. Encyclopedia of Analytical Chemistry. p. 1.

Mikula, Shawn 2016. Progress Towards Mammalian Whole-Brain Cellular Connectomics. Frontiers in Neuroanatomy, Vol. 10, Issue. ,

Brama, Elisabeth Peddie, Christopher J. Wilkes, Gary Gu, Yan Collinson, Lucy M. and Jones, Martin L. 2016. ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy. Wellcome Open Research, Vol. 1, Issue. , p. 26.

Karreman, Matthia A. Hyenne, Vincent Schwab, Yannick and Goetz, Jacky G. 2016. Intravital Correlative Microscopy: Imaging Life at the Nanoscale. Trends in Cell Biology, Vol. 26, Issue. 11, p. 848.

Mikuni, Takayasu Nishiyama, Jun Sun, Ye Kamasawa, Naomi and Yasuda, Ryohei 2016. High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell, Vol. 165, Issue. 7, p. 1803.

Titze, Benjamin and Genoud, Christel 2016. Volume scanning electron microscopy for imaging biological ultrastructure. Biology of the Cell, Vol. 108, Issue. 11, p. 307.

Wacker, Irene Spomer, Waldemar Hofmann, Andreas Thaler, Marlene Hillmer, Stefan Gengenbach, Ulrich and Schröder, Rasmus R. 2016. Hierarchical imaging: a new concept for targeted imaging of large volumes from cells to tissues. BMC Cell Biology, Vol. 17, Issue. 1,

Briggman, Kevin L. 2017. Decoding Neural Circuit Structure and Function. p. 41.

Shinomiya, Kazunori and Ito, Masayoshi 2017. Decoding Neural Circuit Structure and Function. p. 63.

MORGAN, JOSH L. 2017. A connectomic approach to the lateral geniculate nucleus. Visual Neuroscience, Vol. 34, Issue. ,

Li, Xixia Ji, Gang Chen, Xi Ding, Wei Sun, Lei Xu, Wei Han, Hua and Sun, Fei 2017. Large scale three-dimensional reconstruction of an entire Caenorhabditis elegans larva using AutoCUTS-SEM. Journal of Structural Biology, Vol. 200, Issue. 2, p. 87.

Download full list

Article contents

Development of High-Throughput, High-Resolution 3D Reconstruction of Large-Volume Biological Tissue Using Automated Tape Collection Ultramicrotomy and Scanning Electron Microscopy

Extract

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests