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Molecular identification of pathogenicity genes and ERIC types in Vibrio cholerae O1 epidemic strains from Mozambique

Published online by Cambridge University Press:  28 August 2001

E. FOLGOSA ,
S. MASTRANDREA ,
P. CAPPUCCINELLI ,
S. UZZAU ,
P. RAPPELLI ,
M. J. BRIAN  and
M. M. COLOMBO
E. FOLGOSA
Affiliation:
Department of Microbiology, Faculty of Medicine, University E. Mondlane, Mozambique
S. MASTRANDREA
Affiliation:
Clinic of Infectious Diseases, Faculty of Medicine, University of Sassari, Italy
P. CAPPUCCINELLI
Affiliation:
Department of Biomedical Sciences, Faculty of Medicine, University of Sassari, Italy
S. UZZAU
Affiliation:
Department of Biomedical Sciences, Faculty of Medicine, University of Sassari, Italy
P. RAPPELLI
Affiliation:
Department of Biomedical Sciences, Faculty of Medicine, University of Sassari, Italy
M. J. BRIAN
Affiliation:
Maputo Central Hospital, Mozambique
M. M. COLOMBO
Affiliation:
Department of Cellular and Developmental Biology, Faculty of Sciences, University of Rome, Italy
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Abstract

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The phenotypic and genotypic profiles of the V. cholerae strains causing the Mozambican 1997–8 epidemic were characterized to provide a reference for comparison with other epidemic strains. A total of 75 strains of V. cholerae O1 isolated in different provinces, were analysed. Strains were characterized by PCR for detecting toxin genes (ctxA, zot and ace), virulence associated genes (tcpA, nanH, hlyA and toxR) and ERIC sequences. All V. cholerae strains were serotype O1, Ogawa, biotype El Tor. MIC testing showed a high proportion of strains multi-resistant to drugs (100% to cotrimoxazole and 52% to tetracycline) and susceptibility to ciprofloxacin. The isolates contained two intact copies of the CTX genetic element and all other genes tested. PCR of restricted DNA revealed two ERIC types: the first in provincial isolates, also predominant in other African epidemic strains, and the second in Maputo isolates (the national capital).

Type
SHORT PAPER
Information
Epidemiology & Infection , Volume 127 , Issue 1 , August 2001 , pp. 17 - 25
Copyright
2001 Cambridge University Press