Parasitology

Research Article

Association of MMP-2 and MMP-9 with clinical outcome of neurocysticercosis

AVANTIKA VERMAa1, KASHI N. PRASADa1 c1, KISHAN K. NYATIa1, SATYENDRA K. SINGHa1, ALOUKICK K. SINGHa1, VIMAL K. PALIWALa2 and RAKESH K. GUPTAa3

a1 Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India

a2 Department of Neurology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India

a3 Department of Radiodiagnosis, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India

SUMMARY

Matrix metalloproteinases (MMPs) are the major endopeptidases involved in proteolysis of blood brain barrier (BBB) during central nervous system (CNS) infections. The present study detected serum levels and activities of MMP-2 and MMP-9 in patients with neurocysticercosis (NCC) and their association with symptomatic disease. In total, 68 individuals with NCC (36 symptomatic patients with active seizures and 32 asymptomatic individuals) and 37 healthy controls were enrolled for the study. Serum MMP-2 and MMP-9 levels and their activities were measured by ELISA and gel zymography respectively. Mean serum MMP-2 levels (ng/ml) were higher both in asymptomatic and symptomatic NCC cases compared to healthy controls. However, significantly higher levels of serum MMP-9 (ng/ml) were detected only in symptomatic NCC patients compared to asymptomatic NCC cases and healthy controls. Levels of both MMPs positively correlated with symptomatic NCC. Serum MMP-2 activities were significantly higher in symptomatic and asymptomatic NCC compared to healthy controls whereas serum MMP-9 activity was significantly associated with symptomatic NCC compared to healthy controls and asymptomatic NCC. In conclusion, the elevated level of MMP-9 in serum appears to play an important role in the development of symptoms i.e. active seizures in patients with NCC. However, further studies are needed to elucidate its precise role in disease pathogenesis.

(Received April 20 2011)

(Revised June 17 2011)

(Accepted June 26 2011)

(Online publication August 04 2011)

Correspondence:

c1 Corresponding author: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226 014, India. Tel: +91 522 2494265. Fax: +91 522 2668100/+91 522 2668017. E-mail: knprasad@sgpgi.ac.in

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