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An evaluation of melarsomine hydrochloride efficacy for parasitological cure in experimental infection of dairy cattle with Trypanosoma evansi in Thailand

Published online by Cambridge University Press:  18 July 2011

MARC DESQUESNES
Affiliation:
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier, F-34000France Faculty of Veterinary Medicine, Kasetsart University, Chatuchak, Bangkok, 10900Thailand
KETSARIN KAMYINGKIRD
Affiliation:
Faculty of Veterinary Medicine, Kasetsart University, Chatuchak, Bangkok, 10900Thailand
TIMOTHÉE VERGNE
Affiliation:
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier, F-34000France
NACHAI SARATAPHAN
Affiliation:
Department of Livestock Development (DLD)/Bureau of Biology for Livestock Production (BBLP), Pathumthani, Thailand
RODTIAN PRANEE
Affiliation:
Department of Livestock Development (DLD) Chang Mai, Thailand
SATHAPORN JITTAPALAPONG*
Affiliation:
Faculty of Veterinary Medicine, Kasetsart University, Chatuchak, Bangkok, 10900Thailand
*
*Corresponding author: Faculty of Veterinary Medicine, Kasetsart University, Chatuchak, Bangkok, 10900Thailand. Tel: +66 (0) 8 49 40 72 68. Fax: +66 (0) 29 42 86 84. E-mail: fvetspj@yahoo.com

Summary

Melarsomine hydrochloride can cure Trypanosoma evansi infection in camels at a dose of 0·25 mg/kg, but at that dose relapses occur in cattle. In our study, the efficacy of an intramuscular injection of melarsomine hydrochloride at 0·5 mg/kg was assessed in 3 normal and 3 splenectomized dairy cattle experimentally infected with a stock of T. evansi from Thailand. The animals were monitored for 5 months by haematocrit centrifugation, blood- or cerebrospinal fluid-mouse inoculation, polymerase chain reaction, the card agglutination test (CATT) for T. evansi, and the enzyme-linked immunosorbent assay‑T. evansi. Parasitological and DNA tests became and remained negative just after treatment. By the end of the experiment, CATT was negative and ELISA scores were below or very close to the cut-off value. One of the splenectomized cattle died from anaplasmosis during the experiment, but tested negative for surra. It was concluded that the parasites had been cleared from the cattle, and melarsomine hydrochloride at 0·5 mg/kg can be recommended for treatment against T. evansi infection in dairy cattle in Thailand. Further work is necessary to validate the efficacy of the treatment in the event of confirmed CSF-infection.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2011

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References

REFERENCES

Ancelle, T. (2002). Statistique, Épidemiologie. Collection “Sciences Fondamentales” (directed by d’Ivernois, J.-F.), Editions Maloine, Paris, France.Google Scholar
Bajyana Songa, E. and Hamers, R. (1988). A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1/2 of Trypanosoma evansi. Annales de la Société Belge de Médecine Tropical 68, 233240.Google Scholar
Chobjit, S., Kheowsri, S., Rodtian, P., Jongchansittoe, P. and Nuangmek, A. (2006). An outbreak of bovine surra in Maehongsorn Province. Chiang Mai Veterinary Journal 4, 127136.Google Scholar
Dargantes, A. P., Mercado, R. T., Dobson, R. J. and Reid, S. A. (2009). Estimating the impact of Trypanosoma evansi infection (surra) on buffalo population dynamics in southern Philippines using data from cross-sectional surveys. International Journal for Parasitology 39, 11091114.CrossRefGoogle ScholarPubMed
Desquesnes, M. (2004). Livestock trypanosomoses and their vectors in Latin America, CIRAD-EMVT publication, OIE, Paris, ISBN 92-9044-634-X; 174. http://www.oie.int/doc/ged/D9818.PDF.Google Scholar
Desquesnes, M., Bosseno, M. F. and Breniere, S. F. (2007). Detection of Chagas infections using Trypanosoma evansi crude antigen demonstrates high cross-reactions with Trypanosoma cruzi. Infection Genetic and Evolution 7, 457462.CrossRefGoogle ScholarPubMed
Desquesnes, M., Kamyingkird, K., Pruvot, M., Kengradomkij, C., Bossard, G., Sarataphan, N. and Jittapalapong, S. (2009). Antibody-ELISA for Trypanosoma evansi: Application in a serological survey of dairy cattle, Thailand, and validation of a locally produced antigen. Preventive Veterinary Medicine 90, 233241.CrossRefGoogle Scholar
Desquesnes, M. and Tresse, L. (1996). Evaluation de la sensibilité du test de WOO pour la détection de Trypanosoma vivax. Revue d'élevage et de Médecine Vétérinaire des Pays Tropicaux 49, 315321.Google Scholar
Dia, M. and Desquesnes, M. (2007). Infections expérimentales de bovins par Trypanosoma evansi: pathogénicité et efficacité du traitement au Cymelarsan®. Revue Africaine de Santé et de Productions Animales. 5, 3741.Google Scholar
Dobson, R. J., Dargantes, A. P., Mercado, R. T. and Reid, S. A. (2009). Models for Trypanosoma evansi (surra), its control and economic impact on small-hold livestock owners in the Philippines. International Journal for Parasitology 39, 11151123.CrossRefGoogle ScholarPubMed
Eisler, M. C., Brandt, J., Bauer, B., Clausen, P. H., Delespaux, V., Holmes, P. H., Ilemobade, A., Machila, N., Mbwambo, H., McDermott, J., Mehlitz, D., Murilla, G., Ndung'u, J. M., Peregrine, A. S., Sidibe, I., Sinyangwe, L. and Geerts, S. (2001). Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle. Veterinary Parasitology 97, 171182.CrossRefGoogle ScholarPubMed
Gill, B. S. (1977). Trypanosomes and Trypanosomiases of Indian Livestock. Indian Council of Agricultural Research (ICAR), New Delhi, India.Google Scholar
Gutierrez, C., Desquesnes, M., Touratier, L. and Buscher, P. (2010). Trypanosoma evansi: recent outbreaks in Europe. Veterinary Parasitology 174, 2629.CrossRefGoogle ScholarPubMed
Hin-On, W., Rodtian, P., Nuangmek, A., Uthaiwan, V. and Muangyai, M. (2004). Surra in timber elephant from Northern Thailand; diagnosis and treatment; a case study. In: Proceedings of the 30th Annual Conference of the Thai Veterinary Medical Association, Bangkok, Thailand, 10–12 November 2004, pp. 373385.Google Scholar
Holland, W. G., My, L. N., Dung, T. V., Thanh, N. G., Tam, P. T., Vercruysse, J. and Goddeeris, B. M. (2001). The influence of Trypanosoma evansi infection on the immuno-responsiveness of experimentally infected water buffaloes. Veterinary Parasitology 102, 225234.CrossRefGoogle ScholarPubMed
Holland, W. G., Thanh, N. G., Do, T. T., Sangmaneedet, S., Goddeeris, B. and Vercruysse, J. (2005). Evaluation of diagnostic tests for Trypanosoma evansi in experimentally infected pigs and subsequent use in field surveys in North Vietnam and Thailand. Tropical Animal Health and Production 37, 457467.CrossRefGoogle ScholarPubMed
Indrakamhang, P. (1998). Trypanosoma evansi infection in livestock in Thaïland. Journal of Protozoological Research 8, 153161.Google Scholar
Kashiwazaki, Y., Pholpark, M., Polsar, C. and Pholpark, S. (1998). Haemoparasite infections in newly introduced dairy cattle in Loei Province, Thailand: Trypanosoma evansi antigen levels by ELISA referring to abortion. Veterinary Parasitology 80, 99109.CrossRefGoogle ScholarPubMed
Laha, R. and Sasmal, N. K. (2009). Detection of Trypanosoma evansi infection in clinically ill cattle, buffaloes and horses using various diagnostic tests. Epidemiology and Infection 13.Google ScholarPubMed
Löhr, K. F., Pholpark, S., Siriwan, P., Leesirikul, N., Srikitjakarn, L. and Staak, C. (1986). Trypanosoma evansi infection in buffaloes in North-East Thailand. II. Abortions. Tropical Animal Health and Production 18, 103108.CrossRefGoogle ScholarPubMed
Luckins, A. G. (1988). Trypanosoma evansi in Asia. Parasitology Today 4, 137142.CrossRefGoogle ScholarPubMed
Lun, Z.-R., Min, Z.-P., Huang, D., Liang, J.-X., Yang, X.-F. and Huang, Y.-T. (1991). Cymelarsan in the treatment of buffaloes naturally infected with Trypanosoma evansi in South China. Acta Tropica 49, 233236.CrossRefGoogle ScholarPubMed
Masiga, D. K., Smyth, A. J., Hayes, P., Bromidge, T. J. and Gibson, W. C. (1992). Sensitive detection of trypanosomes in tsetse flies by DNA amplification. International Journal for Parasitology 22, 909918.CrossRefGoogle ScholarPubMed
Onah, D. N., Hopkins, J. and Luckins, A. G. (1997). Effects of Trypanosoma evansi on the output of cells from a lymph node draining the site of Pasteurella haemolytica vaccine administration. Journal of Comparative Pathology 117, 7382.CrossRefGoogle ScholarPubMed
Panyim, S., Viseshakul, N., Luxananil, P., Wuyts, N. and Chokesajjawatee, N. (1993). A PCR method for highly sensitive detection of Trypanosoma evansi in blood samples. Proceedings of EEC Contractants Workshops. Resistance or Tolerance of Animals to Diseases and Veterinary Epidemiology and Diagnostic Methods, Rethymno, Greec, 2–6 November 1992; edited by CIRAD-EMVT, pp. 138143.Google Scholar
Payne, R. C., Sukanto, I. P., Partoutomo, S. and Jones, T. W. (1994 a). Efficacy of Cymelarsan treatment of suramin resistant Trypanosoma evansi in cattle. Tropical Animal Health and Production 26, 9294.CrossRefGoogle ScholarPubMed
Payne, R. C., Sukanto, I. P., Partoutomo, S., Jones, T. W., Luckins, A. G. and Boid, R. (1994 b). Efficacy of Cymelarsan in Friesian Holstein calves infected with Trypanosoma evansi. Tropical Animal Health and Production 26, 219226.CrossRefGoogle ScholarPubMed
Pholpark, S., Pholpark, M., Polsar, C., Charoenchai, A., Paengpassa, Y. and Kashiwazaki, Y. (1999). Influence of Trypanosoma evansi infection on milk yield of dairy cattle in northeast Thailand. Preventive Veterinary Medicine 42, 3944.CrossRefGoogle ScholarPubMed
Pholpark, S., Pholpark, M., Sarataphan, N., Khunpasi, S. and Taboran, P. (1984). Trypanosoma evansi infection in buffalo in Northeast Thailand. 2 Experimental study. In Proceedings of the 11th Annual Conference of the Thailand Veterinary Medical Association, Bangkok, Thailand, 4–6 November 1984, pp. 4445.Google Scholar
Reid, S. A. (2002). Trypanosoma evansi control and containment in Australasia. Trends in Parasitology 18, 219224.CrossRefGoogle ScholarPubMed
Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual (3rd Edn.) Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York, USA.Google Scholar
Sarataphan, N., Nishikawa, H., Tuntasuvan, D., Sukhapesna, V., Markvishitr, K. and Satjapitak, S. (1989). Effects of natural Trypanosoma evansi infection on milk yield of dairy cattle in Thailand. Proceedings of the First Symposium on Ruminant Reproduction and Parasitology, Chiang Maï, Thailand, 27–29 November, pp. 331343.Google Scholar
Schillinger, D. and Röttcher, D. (1986). Traitement de l'infection cameline due à Trypanosoma evansi (surra). Revue mondiale de Zootechnie 2632.Google Scholar
Singla, L. D., Juyal, P. D. and Sharma, N. S. (2010). Immune responses to haemorrhagic septicaemia (HS) vaccination in Trypanosoma evansi infected buffalo-calves. Tropical Animal Health and Production 42, 589595.CrossRefGoogle ScholarPubMed
Tuntasuvan, D. and Luckins, A. (1998). Status of Surra in livestock in Thailand. Journal of Protozoological Research 8, 162170.Google Scholar
Tuntasuvan, D., Jarabrum, W., Viseshakul, N., Mohkaew, K., Borisutsuvan, S., Theeraphan, A. and Kongkanjana, N. (2003 a). Chemotherapy of surra in horses and mules with diminazene aceturate. Veterinary Parasitology 110, 227233.CrossRefGoogle ScholarPubMed
Tuntasuvan, D., Trongwanichnam, K., Sukruen, A., Borisutsuvan, S., Mohkaew, K. and Chompoochan, T. (2003 b). Efficacy of Diminazene Aceturate on the treatment of trypanosomosis in pigs. Journal of the Thai Veterinary Medicine Association 54, 4955.Google Scholar
Whitelaw, D. D., Gardiner, P. R. and Murray, M. (1988). Extravascular foci of Trypanosoma vivax in goats: the central nervous system and aqueous humour of the eye as potential sources of relapse infections after chemotherapy. Parasitology 97, 5161.CrossRefGoogle ScholarPubMed
Woo, P. T. K. (1970). The haematocrit centrifuge technique for diagnosis of African trypanosomiasis. Acta Tropica 27, 384386.Google ScholarPubMed
Zweygarth, E., Ngeranwa, J. and Kaminsky, R. (1992). Preliminary observations on the efficacy of mel Cy (Cymelarsan) in domestic animals infected with stocks of Trypanosoma brucei brucei and T. b. evansi. Tropical Medicine and Parasitology 43, 226228.Google ScholarPubMed