British Journal of Nutrition

Nutritional Immunology

Dietary fish oil and curcumin combine to modulate colonic cytokinetics and gene expression in dextran sodium sulphate-treated mice

Qian Jiaa1a2, Ivan Ivanova3a4, Zlatomir Z. Zlateva5, Robert C. Alaniza6, Brad R. Weeksa7, Evelyn S. Callawaya1, Jennifer S. Goldsbya1, Laurie A. Davidsona1a4, Yang-Yi Fana1a2, Lan Zhoua8, Joanne R. Luptona1a4, David N. McMurraya1a4a6 and Robert S. Chapkina1a2a4 c1

a1 Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, TX, USA

a2 Vegetable Fruit Improvement Center, Texas A&M University, College Station, TX, USA

a3 Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA

a4 Center for Environmental and Rural Health, Texas A&M University, College Station, TX 77843-2253, USA

a5 Department of Mathematics and Informatics, Sofia University “Kl. Ohrdiski”, Sofia, Bulgaria

a6 Department of Microbial and Molecular Pathogenesis, Texas A&M University Health Science Center, College Station, TX 77840, USA

a7 Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA

a8 Department of Statistics, Texas A&M University, College Station, TX, USA

Abstract

Both fish oil (FO) and curcumin have potential as anti-tumour and anti-inflammatory agents. To further explore their combined effects on dextran sodium sulphate (DSS)-induced colitis, C57BL/6 mice were randomised to four diets (2 × 2 design) differing in fatty acid content with or without curcumin supplementation (FO, FO+2 % curcumin, maize oil (control, MO) or MO+2 % curcumin). Mice were exposed to one or two cycles of DSS in the drinking-water to induce either acute or chronic intestinal inflammation, respectively. FO-fed mice exposed to the single-cycle DSS treatment exhibited the highest mortality (40 %, seventeen of forty-three) compared with MO with the lowest mortality (3 %, one of twenty-nine) (P = 0·0008). Addition of curcumin to MO increased (P = 0·003) mortality to 37 % compared with the control. Consistent with animal survival data, following the one- or two-cycle DSS treatment, both dietary FO and curcumin promoted mucosal injury/ulceration compared with MO. In contrast, compared with other diets, combined FO and curcumin feeding enhanced the resolution of chronic inflammation and suppressed (P < 0·05) a key inflammatory mediator, NF-κB, in the colon mucosa. Mucosal microarray analysis revealed that dietary FO, curcumin and FO plus curcumin combination differentially modulated the expression of genes induced by DSS treatment. These results suggest that dietary lipids and curcumin interact to regulate mucosal homeostasis and the resolution of chronic inflammation in the colon.

(Received October 25 2010)

(Revised January 10 2011)

(Accepted January 11 2011)

(Online publication March 15 2011)

Correspondence:

c1 Corresponding author: Dr R. S. Chapkin, fax +1 979 862 2378, email r-chapkin@tamu.edu

Footnotes

Abbreviations: COX, cyclo-oxygenase; DSS, dextran sodium sulphate; FO, fish oil; FO-cur, fish oil plus curcumin; IBD, inflammatory bowel diseases; iNOS, inducible nitric oxide synthase; MO, maize oil; MO-cur, maize oil plus curcumin; TUNEL, terminal deoxynucleotidyl transferase-mediated uridine 5′triphosphate-biotin nick-end labelling