Journal of Helminthology

Regular research papers

The development of rediae of Fasciola hepatica in Radix natalensis subjected twice to bimiracidial exposures

Y. Dara1a2, P. Vignolesa2, G. Dreyfussa2 c1 and D. Rondelauda2

a1 Department of Zoology, Faculty of Science, University of Tanta, Tanta, Egypt

a2 UPRES EA no. 3174, Faculties of Medicine and Pharmacy, 87025 Limoges, France

Abstract

Experimental infections of Egyptian Radix natalensis with a French isolate of Fasciola hepatica (each snail was subjected twice to a bimiracidial exposure) were carried out to determine how many sporocysts grew in these snails and to study the developmental patterns of redial generations. Single-sporocyst infections were found in 69.3% (34/49) of infected snails, with equivalent numbers of normal and abnormal patterns. Snails with two- and three-sporocyst infections were 24.4% and 6.1%, respectively. In single- and two-sporocyst infections at days 42 and 56 post-exposure, the total redial burden was significantly higher in snails with a normal redial development. In two- and three-sporocyst infections, the overall maturity of rediae was delayed at days 42 and 56. The high frequency of abnormal patterns in R. natalensis (53.1% of all infected snails showed degeneration of a first mother redia) might be due to incomplete adaptation between the snail population and the parasite. The delayed redial maturity in two- and three-sporocyst infections can mainly be explained by the volume of the snail body, which would be insufficient to allow the simultaneous differentiation of most rediae over time.

(Accepted July 18 2010)

(Online publication September 08 2010)

Correspondence:

c1 Fax: 33 555 435863 E-mail: gilles.dreyfuss@unilim.fr